FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGG15.263157894736842No Hit
GAACATTACTTTCAAAAATGTCTAGACAAATACTGCAATGCCATTCGGGA15.263157894736842No Hit
GAATGGTATGGCGGAATCTGATTTCGCAGCTGCGGAAATATTGCGGAAAT15.263157894736842No Hit
GTGGTGTTGCCAGTCAAAGACCCGAGAACCAGGTTGTTCGATTCAGCACG15.263157894736842No Hit
ATGAGTAGGAGGACGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG15.263157894736842No Hit
GTGCTCTTCCGATCTCTCGTGGGAGACGTGTGCTCTTCCGATCTCCTTTG15.263157894736842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG15.263157894736842No Hit
TATGGGAACTGGTTCAGTCGTTGGACAACGAAAAGATCTGCTACGACCAC15.263157894736842No Hit
CTAGATTTCATTTTCTTTCTTTCTTTTTGTTCCCCAAGGTCCCTAGTTAA15.263157894736842No Hit
CTTCCGATCTGAGTAGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGA15.263157894736842No Hit
GACTGCTACTTACTAACCGACTCATCTGTACTTCCTTGGACACGCTTCTC15.263157894736842No Hit
AAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATT15.263157894736842No Hit
TCTCCCGGCGAGATCGGAAGAGCACACGTCTCCCCGGAGAGATCGGAAGA15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15.263157894736842No Hit
ACCGCCACGTCCCGGTTCCGGAATTTTAACCGGATCCCCTTTCGAAGTTC15.263157894736842No Hit
CAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGATTCCGTGTGA15.263157894736842No Hit
GATCAAAGGAGCATTGCTTTTGTGGCTAGTTTAGCATTGTTACTATGATG15.263157894736842No Hit
GGGTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTC15.263157894736842No Hit
TACAGTCAGAGGTTCAATTCCTCTTCTTAACACCTGGCAGAGCCCGGGTC15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)