FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCCCTGAGCAGGACCAGGAGCTTCAGTCGGAGAATCGGAGATCGC11.1904761904761905No Hit
CACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTA11.1904761904761905No Hit
CTTATAATGCACATTGCATTCTTCTTCCTCACTTTCTTCTTCATGATTCC11.1904761904761905No Hit
ACCCGGTACCTTGGCTCAAGTTGTTGGAAAAGCTACCGTTGTTCACCAGG11.1904761904761905No Hit
TTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACT11.1904761904761905No Hit
GGCGAGAGCGGGTCGCCGCGTGCCGGCCGGGGGACGGACTGGGAACGGCT11.1904761904761905No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT11.1904761904761905No Hit
GAGTAGCATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTT11.1904761904761905No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11.1904761904761905No Hit
CTACCCAGGGGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAA11.1904761904761905No Hit
ACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAAT11.1904761904761905No Hit
ATTCCATGTGATGCAATAAGTGTGAAAGTTAACAGTTGGGTCGAACCATA11.1904761904761905No Hit
TCCATCATTTGCTTGACATGATCCATGGCGGCCAAATTGAAACCGATTGT11.1904761904761905No Hit
GCCGTCTTACTTCACCGCTTCCGATTCAGGTCTCGAATTCGACGAATCCG11.1904761904761905No Hit
CTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGATGCGTCGC11.1904761904761905No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT11.1904761904761905No Hit
CTTCCGATCTGTCTGGGGAGACGTGTGCTCTTCCGATCTGTCTGGGGAGA11.1904761904761905Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TATCGGAATGGATTATTGGAGCGGTCATGGGAATGTTTCTGGAGCAGTTC11.1904761904761905No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT11.1904761904761905No Hit
GATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGATAA11.1904761904761905No Hit
TCCCAAGCTAGATCGGAAGAGCACACGTCCCAAGCTAGATCGGAAGCTCA11.1904761904761905No Hit
CGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGCTCAGGCAT11.1904761904761905No Hit
AATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGT11.1904761904761905No Hit
CTGATGTTGAGATCATCACCTCCGGTCGTCGTAGTATTCCGGCACACTCC11.1904761904761905No Hit
GATGAACCCGGTTCATAAGAAAATTCCGGTTCTCATCCACAATGGTAAAC11.1904761904761905No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTC11.1904761904761905No Hit
AGCTATTACAGCTTGTTTGACCTTAGAATGTAGCTCATTATAAACCAGGT11.1904761904761905No Hit
CATCTGCACGCCCATCTTTACGACAAGTTCAATGATGATTATGGTAGCCA11.1904761904761905No Hit
TTATATGGCTCCTGAAGTATTCAAGCATAGAAGATATGACAAGAAAGTCG11.1904761904761905No Hit
ATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCGCTGA11.1904761904761905No Hit
GAGTGTGCTTGTTTCTGTTCATCAATGGAACTCTGAGCCGCTTGAAGCTC11.1904761904761905No Hit
GTGTAGAGATTGCGGACACTCTTGTCTGCATCAGGGGATAAATATCTTCC11.1904761904761905No Hit
CCAATGGAACCTCGTTAAAGGATTTAGATTGTACTCATTCCAATTACCAG11.1904761904761905No Hit
AACTCGTAGTGTGCAGCTGAAAACTTCTGATTCATTCCACCACCACTGTT11.1904761904761905No Hit
GGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAA11.1904761904761905No Hit
ATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTC11.1904761904761905No Hit
GAGTAAACGAAGCATGCGGTCCAACAAGACCGGAGCCCCAGGGTTACGGG11.1904761904761905No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTTG11.1904761904761905No Hit
TCCGGTGGGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATT11.1904761904761905No Hit
CTCATAAGGTGCCAGCGGAGTCCTATACGCAACATCCGCTGATCCCTGGT11.1904761904761905No Hit
TCTGAGGAGTTGTTTGCCGCATCTGTGATGTTTACAGGTTGCTGATTCTC11.1904761904761905No Hit
CCCTTCAACATGGCTGCCTTCAAGAAGAAGAGCCCCCACAACAGCCTCTT11.1904761904761905No Hit
TGTCGATCGTGAAAAGACATGTCCCCTTCTTCTTCGTGTTTTCACCAAGA11.1904761904761905No Hit
CAATAGGGGCTTCTTCATTAGCAGATTAAACAAATCTCAACATTCGAATT11.1904761904761905No Hit
CCAAAGGCGGACCGGCCGACCCATCCCAAGGTTCAACTACGAGCTTTTTA11.1904761904761905No Hit
GAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATT11.1904761904761905No Hit
AGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGC11.1904761904761905No Hit
ACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCTACGCTCTGTATACATTA11.1904761904761905No Hit
GTATATACTGGTAGAGATCATCGCTCTGTAAGAGACTCTTGTGACCAACT11.1904761904761905No Hit
GTGCGGGCGCCGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTA11.1904761904761905No Hit
TAGTGGAACGAATCAACGCCCAATCATTACCCACCACGTGTGAACAAGTG11.1904761904761905No Hit
AACTATGGCTGAGGAGTACAAGAACAACGTTCCCGAGCACGAGACACCAA11.1904761904761905No Hit
GGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAA11.1904761904761905No Hit
CCTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCT11.1904761904761905No Hit
CTTCCGATCTCACGGGGGCATATTTTTCACGGGTGACGGAACTGGTCAAG11.1904761904761905No Hit
CTGCAAAGGGTTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT11.1904761904761905No Hit
GCCGTGCTTCTGGATCGCGTGGGCCGCGTCGATCGTCACCTGGTCGTCGG11.1904761904761905No Hit
ACAATAATGAGTGAAACTAGGCTGAATGAAAGGAAATGAGGGCAGGCAGC11.1904761904761905No Hit
GATTCAAGGCGTATATCAACGTTTGGCACTGGTGGACCAACTGTGCCGAG11.1904761904761905No Hit
CTACATAGGAGCATTGCTATTTGGAATGATCATCAACATGTTTAATGGGT11.1904761904761905No Hit
ATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAA11.1904761904761905No Hit
GTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGG11.1904761904761905No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAGCGCACACGG11.1904761904761905No Hit
CAATCACACCATTACCATACATAGGAAAATACAAAGCTGGGAAAAGTCAA11.1904761904761905No Hit
ATTAAGACCAGGAGCGTATCGCCGACCGAAGGGACAAGCCGACCAATGCA11.1904761904761905No Hit
GCTATAAACTCAGCAATCCCAGCTCTCCAGAAGGACCATGAAGCTAGCTC11.1904761904761905No Hit
AAATACTACATAGAGTTCAACAATACTAAGATAAAAAGTGCTTCTTCAGA11.1904761904761905No Hit
GGTCTTAATTGGCCGGGTCGTGCCGCCCGCGCCGGTTCTTTTAAGAAAAT11.1904761904761905No Hit
TTTCAAGACTGATTTGCGTTTCCAGAGCCATGCTGTCTTAGCTCTCCAGG11.1904761904761905No Hit
TATATGGACTTTGGGTCATCTACAGCTTCCGGACAAGAGCGACCGATAAA11.1904761904761905No Hit
GATGTACGGACAATTGACCAGATAAGATTGATTGAGCGGAATTGCGAAAT11.1904761904761905No Hit
AGCCTATGGAGCTTCTGGGTCGGAGACTCGAGTGTATACCCGGAAAAAAA11.1904761904761905No Hit
GCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCC11.1904761904761905No Hit
CCCAAACAACCCGACTCGTAGACAGCGTCTCGTGGTGCGACAGGGTCCGG11.1904761904761905No Hit
ATCCAGTTTGGACAGAAAGGCATTCCTTACCTGAACACTTACGATGGTCG11.1904761904761905No Hit
GCCGAAGAGGGGAAAAGTTCCATGTGAACGGCACTTGCACATGGGTTAGT11.1904761904761905No Hit
GAAGGTGAAGACGGTGAGAAGAAGAAGAAGGAGAAAAAGAAGAAGATTGT11.1904761904761905No Hit
AGGAAGAGGTTGAGATGAAGGAACTGGTAGGGCTTGTGAGAATCCTAGTG11.1904761904761905No Hit
TTTTAATTCCTCACCACCTCAAATTCTTCATAAACAAATCCACTTCTGCA11.1904761904761905No Hit
CCTTACTCCGGAATTGCTTCCAGTAGTAGTTGAGTCTCTTCATGATACGG11.1904761904761905No Hit
ATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAG11.1904761904761905No Hit
GGATTGTGGCAGTAGTGACGACGACGCCAAGGAGGATATGGAGGCTGGGA11.1904761904761905No Hit
CCTCTAAGTCAGAATCCGGGCTAGAAGCGACGCATGCGCCCGCCGCCCGA11.1904761904761905No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA11.1904761904761905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAT100.072.57
AGCTAGA100.072.56
CTAGATC100.072.58
CCAAGCT100.072.53
AAGCTAG100.072.55
CAAGCTA100.072.54
TAGATCG100.072.59
TCCCAAG150.048.3333321
CCCAAGC150.048.3333322
GGGGGGG1250.05.7999997125-129