FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACAATATTGTTTATCTTCTTCTGAAATGTGGAATGGATCTTGAGGTCT11.7241379310344827No Hit
AAGATAGGTAGTGTACAGCTGACGGATTCCAAACATTCTTTCTTGAGCTC11.7241379310344827No Hit
GGGTGGAGGTGGAGTTCTCCGTGCCCAAGGTCCCCTACCGGGAAACCCTC11.7241379310344827No Hit
GAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCG11.7241379310344827No Hit
CTTCCGATCTCAATAGGGCGAAAGTCCTCAAATCTCTCCGTCACGAATCG11.7241379310344827No Hit
GCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTG11.7241379310344827No Hit
GCACACGTCTCCCTATGGAGATCGGAAGAGCACACGTCTCCCTATGGAGA11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGGTTGAATTACTATCGCGACGCGGGCATCAGTAGGGTAAAACTAACCTG11.7241379310344827No Hit
GTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAAC11.7241379310344827No Hit
GAAGGTGTACCGTGAGGAGCGCTGGAACGCATAGAAGTGAGAATGCCGGT11.7241379310344827No Hit
TCACAAGGGAGACGTGTGCTCTTCCGATCTCACAAGGGAGACGTGGGGTC11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCACACATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATG11.7241379310344827No Hit
GATTAAATATAGATACTGGTGTTATGAAAGATAGTCAAGGAAACATTTAT11.7241379310344827No Hit
GCTCCGGGCTCTGCTGGTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA11.7241379310344827No Hit
TCCCTGTGTAGATCGGAAGAGCACACGTCTCCCTGTGTAGATCGGAAGAG11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACTTCTTACAAGAAGGTCTCCCTCTTCAAGGACAGTTCCCTGTCTCTTAT11.7241379310344827No Hit
GATCGGAAGAGCACACGTCTCCCAGGCAAGATCGGAAGAGCACACGTCTC11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATG11.7241379310344827No Hit
CCAATGGCAGAGCCCGCGGAAGATCGGAAGAGCACACGTCTCCCCAGCCC11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCTTGGGAGACGTGTGCTCTTCCGATCTTCCTTGGGAGACGTGTTCTCT11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGG11.7241379310344827No Hit
AAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATT11.7241379310344827No Hit
CCACTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGATGCGT11.7241379310344827No Hit
GAGCACACGTCTCCCAAAGCAGATCGGAAGAGCACACGTCTCCCAAAGCA11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAAC11.7241379310344827No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG11.7241379310344827No Hit
CTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACGACGCT11.7241379310344827No Hit
GGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAG11.7241379310344827No Hit
CTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCG11.7241379310344827No Hit
CGTCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAAAAAA11.7241379310344827No Hit
ACCTGATACTAAACAACATTCTGGAGCAGTGAAGACAGGAGTCCCGGGAG11.7241379310344827No Hit
GGCGATAGATACTTGAAACCATCCATCATTCCTGATCCGGAAGTGACGGC11.7241379310344827No Hit
GATGGGGGCCGGCGATGCGTCCTGGTCGGATGCGGAACGGAGCAATCCGG11.7241379310344827No Hit
CTTTTCACCTTTCCCTCACGGTACTTGTTCACTATCGGTCACATGTTAGT11.7241379310344827No Hit
GAGCACACGTCTGGGAGATCGGAAGAGCACACGTCTGGGAGCTCGGAAGA11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAACAGGGGGTGGGAGTAGCGAAAGACCGGGGAGCATCCCGTCCACCGAT11.7241379310344827No Hit
ACCCTCCTCCGTCCGATTTTGATTCCATCTCGATCCCTCCGTTAGATGAT11.7241379310344827No Hit
ATATGAGTGTTCCAAAAGTTCTTGATCTCATTATCTGTTCTTCCTGGTAA11.7241379310344827No Hit
GGAATATAAACCTGTTATCCATCGCCTACGCCTTTCGGCCTTAGCTTAGG11.7241379310344827No Hit
GTGCTCTTCCGATCTTTTTTTTTCTTTTTTTTTTTTTTTTTTTGGGCCGG11.7241379310344827No Hit
ACTTGTTCACTATCGGTCACATGTTAGTATTTAGCCTTAGGGGATGGGCC11.7241379310344827No Hit
CTCAACACCTGTGATTGAAAGAACAACACCTCCAAGTGAAAACCCACCCT11.7241379310344827No Hit
ATATCTAACAATCTGAAAAGTTTAATCAAAAACAAAGACTTTACAATAGT11.7241379310344827No Hit
TTTTCGTGAAGACTCTCACTGGAAAGACCATTACTCTTGAAGTTGAGAGC11.7241379310344827No Hit
GAATAAGATATTCGACACATCAATCACTATAGGTTCTGTCTCTTATACAC11.7241379310344827No Hit
GATCTAAGTAGGGAGACGTGTGCTCTTCCGATCTAAGTAGGGAGACGTGT11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TGCTAGACGTGTGCTCTTCCGATCTGCTAGACGTGTGCTCTTCCGATCTG11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAACCGGTACGGACTTGCAACGTCGCAGAAGTACGACTGTCTCTTGTTCG11.7241379310344827No Hit
ATTGTACTCATTCCAATTACCAGACTCGAAAGAGCCCCGTATTGTTATTT11.7241379310344827No Hit
TCCCAACCCGATTGATGCACTTTGAGCGCCAACTTTTCAATCAAGTTGTG11.7241379310344827No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT11.7241379310344827No Hit
TATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCT11.7241379310344827No Hit
TGCAAGAGGTTCATTAGGAACAAAAAAGACCGGAATTATAGAAGCCGGAG11.7241379310344827No Hit
GGATTAAGGTGTTCTCTCTGACTTCGGAGGAGAAAAAAAGAAAAAAAATA11.7241379310344827No Hit
ATACTATGCACGTAAAGGTTCCAAGCTTCCACAGAGAGAAATGTTCAGGA11.7241379310344827No Hit
TCCATATAGATCAGCATATACGTACCTTCCAGCTATGCAAGGATCAGTTT11.7241379310344827No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA11.7241379310344827No Hit
TGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGC11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTA50.0145.0145
ACTAAAC50.0145.08
GATACTA50.0145.05
CCTGATA50.0145.02
CTGATAC50.0145.03
TGATACT50.0145.04
ACCTGAT50.0145.01
CTAAACA50.0145.09
ATACTAA50.0145.06
TACTAAA50.0145.07