FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATTATCGGATTTGGGAGAAAAGCAGAGTCCATGGATTCCCCGCCGG11.7241379310344827No Hit
AGCTCCAAATAGCGTATATTTAAGTTGTTGCAGTTAAAAGCTCGTAGTTG11.7241379310344827No Hit
TCCGATCTCCTCAGGGAGACGTGTGCTCTTCCGATCTCCTCAGGGAGACG11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAGAAGATTGCCATGTAGGAAACCGCGGAGAAGAAGGCAATAAGAGGA11.7241379310344827No Hit
AGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAAC11.7241379310344827No Hit
ATCATACATTGATACTTCACAAAAGTACATCTTGATATGTCAGATACTAT11.7241379310344827No Hit
GGTGTGCCCTATTAGTGTGTAGTGAACACGAAGCATGAATGGGTTGCAAA11.7241379310344827No Hit
TCATTAGGGTGGCCGGGTCGTGGGGCCGGCGCGGGTTGTTTTTTTTAAAT11.7241379310344827No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT11.7241379310344827No Hit
ATCTTCTCTTCTCCTATCTTAAACACAACATTTTTAGATACACTGCGGTT11.7241379310344827No Hit
GAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCC11.7241379310344827No Hit
CTTCCGATCTGCCTGGGGAGACGTGTGCTCTTCCGATCTGCCTGGGGAGA11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGTTAAAATTCCGGAACCGGGACGTGGCGGTTGACGGCAACGTTAGGGAG11.7241379310344827No Hit
GCATGGTTCTCTTGGTTCCTTCCAAGGAAGAGAGCTAACCGTCACAGAGG11.7241379310344827No Hit
CCCTTGTGAGATCGGAAGAGCACACGTCTCCCCTGGGAGATCGGAAGAGC11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTCTTACAAAACGATTCGAGCTAAAAAAAAAGAGAACAAAAACACGTTCA11.7241379310344827No Hit
AGCGTGGGGGGGAACGCGTTTAGTCACAGCTTTCTTCTCCTTTAGCTTTT11.7241379310344827No Hit
GATTTGGAGAGCCCATGAGTCAGGGATATACTAGGACGTTTGGAATGAGA11.7241379310344827No Hit
ATATGCAGGCCAGTGACGTCCCAGGCTCTGCCCATTTATCCATATTTGAC11.7241379310344827No Hit
CCCTAATTAGTAATTACCCTCTTTTTCTCTTTACACTGAGTTTTCTAATT11.7241379310344827No Hit
GTGTGCTCTTCCGATCTAGCGCGGGAGACGTGTGCTGGTGCCATCAAGCG11.7241379310344827No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCATTGGTGTGCATTGGT11.7241379310344827No Hit
TCTTAACACCAAGTTCAATTCCTCTTCTTAACACCAAGTTCAATTCCTCT11.7241379310344827No Hit
CAATTTCAGCTGCCCTTGCCCTAGCAGTCTCAATTGACTCATCTCCCGAT11.7241379310344827No Hit
GTGTAGGATAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAA11.7241379310344827No Hit
CTTCCGATCTGACCAGGGGTCGCAAGTCGTCTGCAAAGGATTCTACCCGC11.7241379310344827No Hit
ATTGTAACAGAGCTTCTTCTCGGAGCTCTCATTGTTCTTTTCAGGGCAGT11.7241379310344827No Hit
GGAGGGAAGCGGATGGGCGCCGGCGATGCGTCCTGGTCGGATGCGGAACG11.7241379310344827No Hit
CTGTAACGGGAGACGTGTGCTCTTCCGATCACACTGGGGCGGTCGCGTAT11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG11.7241379310344827No Hit
GCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCAT11.7241379310344827No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC11.7241379310344827No Hit
TGTGTCTGTCTTTTATGATCTCTCATAGTTTTTTTCTAAAGTATTTTCAT11.7241379310344827No Hit
CCACTGGGAGACGTGTGCTCTTCCGACCTCCACTGGGAGACGTGTGCTCT11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATGTATTCAACGAGTTCACACCTTGGCCGACAGGCCCGGGTAATCTTTG11.7241379310344827No Hit
CTTTTTAATATCGTTGTTTTGTTCGATCTCATCTCTTTCTTCGGAAAAAA11.7241379310344827No Hit
CGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCG11.7241379310344827No Hit
TTTTCAAGGCGAGATAGGTAGAGCTATATCCTCCTCTTCGCAACCTTCTC11.7241379310344827No Hit
CTGCAGACGAAGTTCTGCTTCTGAATTCCCTTGACAACTACGTGCTGCGC11.7241379310344827No Hit
TCCACGCGTTTTTGTTTGTTGTTAGGCGGTATAGAAGGAACCGATACTCC11.7241379310344827No Hit
GCAGGGAGACGTGTGCTCTTCCGATCTCAGCAGGGAGACGTGTGCTCTTG11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCATCTACTTTAGCGGTAATGTATAGGTATACAACTTAAGCGCCATCCA11.7241379310344827No Hit
ATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAA11.7241379310344827No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.7241379310344827No Hit
CCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCGCGACGG11.7241379310344827No Hit
GGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGATTTATGAAAGA11.7241379310344827No Hit
GTTTATCCCGTGCGTATGGCCAAATGATACTGAAAAGTGGTTTCTTTCAT11.7241379310344827No Hit
GTTTCTTGGGGGGGGGGGGGGGGGGGGCCCCCCGCGGGGGGGGGGGGGGG11.7241379310344827No Hit
GCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAATCAGCGCCGG11.7241379310344827No Hit
TCTCCCTGGTGAGATCGGAAGAGCACACGTCTCCCTGGGGAGATCGGAAG11.7241379310344827Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTGATGTGGGGTAAACAAATTGTTTCCCGCAGCTTCAGATCG11.7241379310344827No Hit
GTGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCG11.7241379310344827No Hit
ATCGACAACGTCAAGGCCAAGATCCAAGACAAAGAGGGTATTCCTCCGGA11.7241379310344827No Hit
GCTTAAATCCTGTCTGTTTCAAACATAGGATATTATGAAACACAAAACTG11.7241379310344827No Hit
ATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGA11.7241379310344827No Hit
AGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCAT11.7241379310344827No Hit
GCATACACCTAAGAAAGCTTCATCTGGTTCGGCCGATAGAGATGTGATAC11.7241379310344827No Hit
AGGCAAGAGTTAGGAGAAGCAGAATTACGAAACACAGATTTGTACAAGGA11.7241379310344827No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGG50.0145.0145
TTCCGAT50.0145.02
CTGACCA50.0145.09
GATCTGA50.0145.06
TCTGACC50.0145.08
TCCGATC50.0145.03
CCGATCT50.0145.04
ATCTGAC50.0145.07
CTTCCGA50.0145.01
CGATCTG50.0145.05
Produced by FastQC (version 0.11.5)