FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCATCCTTAACTCTCAAAGGCAGCCAAACGATCCTCATGAGATC13.8461538461538463No Hit
GATAACATCAGCCAACTTTCTCAACTAAAATGGTTGGACTTGAAGTATTG13.8461538461538463No Hit
ACATTGACCAAAAGGCTAAGAAAAGAAGGAAGAGAGGAAGAGTCTTCAGA13.8461538461538463No Hit
GTTGAAGGCCGCGATAGTCCTCGGGGAGTTGTCAATCGAGCGTTGATCCG13.8461538461538463No Hit
TCAAAAGCCCGGGCTTAGGCCGCCCCCGTAATCCGCGTCGGTCCACGCCC13.8461538461538463No Hit
GCAATGTCGGGTAAGTCGAGTCTGATGTATCAAAAGGTGATGATGACTGT13.8461538461538463No Hit
GGGTAAAAGCTCGTTTGATCCTGATTTTTCAGTACGAATACGAACCATGA13.8461538461538463No Hit
CGCTTAAAGCAATCGGCGACGAGATCTTAATGGATTTGTCGAGATTGAAC13.8461538461538463No Hit
CAATATGAGTAAGCTTCAGAGTGAGGCTGTTAGAGAAGCCATAACGACTA13.8461538461538463No Hit
TATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATA13.8461538461538463No Hit
GTCGATCGGCGGTGCACCCGCGAGGGATCCCGCCAATCAGCTTCCTTGCG13.8461538461538463No Hit
GTTTAAGACAATGTCGTCAAGGCTGTCAGAAACAACAACCTTCACTGGCT13.8461538461538463No Hit
ATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAAT13.8461538461538463No Hit
CACGTCTCCCTGTGCAGATCGGAAGAGCACACGTCTCCCTGTGCAGATCG13.8461538461538463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTTTACAGGGGGGATAACATCATAGGATTTCGATCCT13.8461538461538463No Hit
GAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAATTTCGAAAGGG13.8461538461538463No Hit
CTACAGGGGGACCTCAAAGAAAACATCGAAATCTTAGAGAAGAAGAAAAG13.8461538461538463No Hit
CTCTTGACCTCGTCGTTCAACGAGCTCATCTCCGCCTTATGAATCTCGGC13.8461538461538463No Hit
GTTTCTCTCTTCTCCAATTCCTAAACCCGACATAAACCATCGCCACCATT13.8461538461538463No Hit
CCAAACTCCCCACCTGACAATGTCCTCCGCCCGGATCGACCCGCCGAAGC13.8461538461538463No Hit
CCTTAAACTGGACACTCTCTATCAGTGGAGAAGAGAGTTGATAACACAGT13.8461538461538463No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGC13.8461538461538463No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC13.8461538461538463No Hit
ACCTTCTCATGCCTTGGTACATAATCCTCAAGTTTCTTGACAAGTTCTTT13.8461538461538463No Hit
CTTCACCGGATCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGG13.8461538461538463No Hit
GGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAA13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)