FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAACTCTTACCATTTGCAATCATCTGAAGAACTTTATCCCAATCACC11.694915254237288No Hit
ACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGT11.694915254237288No Hit
ATCTGTCCTGGGGTGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTT11.694915254237288No Hit
TCGACCGACAGGGGCTTACGCTGTTAAGGGTTGTCCTCCCTCGGCGCACC11.694915254237288No Hit
TCACTACTGTGTTTTCGTTTTTGTTTTATCCAAACAAAATCGACAACACA11.694915254237288No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG11.694915254237288No Hit
GATCTGGACAGGGCAAACAGGGTTTCAAGGGCTTTGAAAGCTGGTACCGT11.694915254237288No Hit
GAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCC11.694915254237288No Hit
CACCCATAATCTTCACAAAAGGAGCAAAAAGAAGTTCCAAAATCTCCTTC11.694915254237288No Hit
GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTA11.694915254237288No Hit
CCTTCTGGATAGCGCGCAGGTCTTTGAAGACCTCCATCCAGTCCGTCGGA11.694915254237288No Hit
CTCAAAAGGAATGTCGTTACGGGAAAAGAAGATTGTTTCTTAAGAGAATT11.694915254237288No Hit
ATCAAGTGAGAGATCTTTGCCTTTTCTTACAACAAATCAAATGTTTCTAA11.694915254237288No Hit
GTCAAAAACCTTCTCATTATGCAAAGTGATTTTTGAAACCCTCAAAAGTC11.694915254237288No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11.694915254237288No Hit
CCTTGGCTCAACCTCCCCACGGAAGTACCAAGCCATTCTGATGCCACTAA11.694915254237288No Hit
GATGATTCATGATAACTCGACGGATCGCATGGCCTCTGTGCTGGCGACGC11.694915254237288No Hit
GGCTGTTTTCTGGCAGAACCATTCGGATGTGTGCACCACGATCGAACAAG11.694915254237288No Hit
CCCCTTATCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAAC11.694915254237288No Hit
GCCGTGATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAG11.694915254237288No Hit
ATCTCACAGAGGAAAAAAAAAGTATAAAGAACTTCCAATTCCTCAATTTG11.694915254237288No Hit
CTTCTACCCTTTCAGTGCCTCTTTACTTCATATGATGTTAAAACCAGGTA11.694915254237288No Hit
AATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGC11.694915254237288No Hit
CGGTAAGTGTGACTGTTGTCAAAACACTAGAATCAGTGTCGATTTTAACA11.694915254237288No Hit
TGTATACATGTGCCATATTGGTGTGCTGCACCCATTAACTTGTCATTTAA11.694915254237288No Hit
GCCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGG11.694915254237288No Hit
GGGGCGGGCGTGAGCGGCCCTCGGTGCTCCGGTGTTTCATGGGCCGGCGG11.694915254237288No Hit
AATGAAAAAATAAATAAATAAATACTAAAATAAAGCTATGAAAATCCAGT11.694915254237288No Hit
CCAAACAAACATCTTCATGCTCGGAGCAGTTGTTTTCTTCTCTACATAGT11.694915254237288No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG11.694915254237288No Hit
GTGTAATACTCAATGCTGTCTACTTGTTTACCGACTAGCCCACAGAAGCA11.694915254237288No Hit
GTTAGAGACCAGCCCGGGCCACATCTCGAAAACCCATCTCTACAAAAAAA11.694915254237288No Hit
GTCCTGGGGTGGGGGTACGTACCTATTTATTATTCGTCGCCGGTCCGGCT11.694915254237288No Hit
GACTATTACCATTACACACCAGAGGAAATCAAGTCATTGTGGCATGACAA11.694915254237288No Hit
TCACTGGGGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGT11.694915254237288No Hit
GGTTAGCGTTCGCGCGAAGCTCTGAATTGAAGCCCCAGTAAACGGCGGCC11.694915254237288No Hit
GGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCT11.694915254237288No Hit
GACTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGA11.694915254237288No Hit
GATGCTGTCTCTGAAGACACCCTCGTCAAAGTTATGACAGACGGTATCCT11.694915254237288No Hit
GACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAA11.694915254237288No Hit
GTGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11.694915254237288No Hit
CTTGACCTCGTGATCCGCCCACCTCGGTCTCTCAAAGTGCTAGGATTACA11.694915254237288No Hit
CTATGCTGAGAGCTGATAGCAAGCTAGTTTACTAGCGAAGTGGCTGATAC11.694915254237288No Hit
GCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGT11.694915254237288No Hit
GTGGGTGGTAACGCGGGGAAGTGAAACATCTTAGTACCCGTAGGAAGAGA11.694915254237288No Hit
TTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTAC11.694915254237288No Hit
CTCTTACCTCTCACTTCCACCTCTCCTCCACCGTAACCCCCACCGTCGAT11.694915254237288No Hit
CAAACTGTACTCGCGGTAGTAAATAAGTCTCATGAGATTGGATGGTTTCA11.694915254237288No Hit
TCTTAATGCAAAATCTTAGAAGTAGATTTCTTGGGTCAAATGTTTTGAGT11.694915254237288No Hit
CATGAAAAGTATGATAAATGCTTAGTGAATAGGACATCCTAAGGAAGAGT11.694915254237288No Hit
ACCTTGTCTCGTTGGTAAGTATGCCCTGAAAAATATTGTGCACCCATGTA11.694915254237288No Hit
AAACACACCAGGGTGGTATTTCACCGTTGCCTCCACCGAGCCCAAGAGCA11.694915254237288No Hit
GGGCAGAACCAGCAGCAGGGGCTGCAGAGCCTGGGGCAGCAGAGACGGCT11.694915254237288No Hit
TTTCTATAGCGGTGGAAACGGCGGTGGAGGAGGCGAAGTAGACGAGAGCG11.694915254237288No Hit
TATTTAGACAGCACCCGATTAGAGCTCTTTAGAGCGCTTTTCTGATACAT11.694915254237288No Hit
GTGTTTCGAGATTGTCACCCTCTTCATTGGTGTGCCGCTGCTCGGATATG11.694915254237288No Hit
GTCCACGGCTCATCGCCGTCACGCACCGGCAGGGATGCAGCAATCGCCTC11.694915254237288No Hit
TAGTTGAACCTTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGGGT11.694915254237288No Hit
ATCTACACCGGGCTCTGGTGTTAAGAAGAGGAATTGAACCTCTGACTGTA11.694915254237288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCA50.0145.04
AAACACA50.0145.01
ACACCAG50.0145.05
ACACACC50.0145.03
CAGGGTG50.0145.09
CACCAGG50.0145.06
GGGGAAA50.0145.0145
CCAGGGT50.0145.08
ACCAGGG50.0145.07
AACACAC50.0145.02
Produced by FastQC (version 0.11.5)