FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7F8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7F8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACCTGTTATTGCCTCAAACTTCCATTGGCTGGAAGCCAATAGTCCCT218.181818181818183No Hit
TTGCTATTCTATATCCAGCCAGTAACATGCTGTATCCTTTTTGAATTATA19.090909090909092No Hit
CGTGTGCTCTTCCGATCTTGACTGGGGATACTTGTGCTAATCCGATCTTG19.090909090909092No Hit
CATTACCATCGTAGCGAGACGTCTGTCCAATACCGCTTCGGAAGCGGGAC19.090909090909092No Hit
GAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTGTCGGGAGG19.090909090909092No Hit
TCACCATGTCGCGTGCGATCTGGGTCGCGCGCTCGAAGTCGTTCGAGGCG19.090909090909092No Hit
TCTCCACAGGGAGACGTGTGCTCTTCCGATCTCCACAGGGAGACGTGTGC19.090909090909092Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCTACGAGTCGGGGTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT19.090909090909092No Hit
CAATAGATGCAGAAAAATTATGACATAATTCAACCTACTTTCATAATAAA19.090909090909092No Hit
GAACACCACACCCAGAAGAGATCTGAACAGAACACCACACCCTGATCATA19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)