FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7E9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7E9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC14.0No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA14.0No Hit
GAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGG14.0No Hit
CACAAAGGACAACTCAGGTGCATACCTCAGATGAAAAAGCAGCGGATTTG14.0No Hit
GTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTGTTGATATGC14.0No Hit
GACCCCCGCAATCCTAAATCATAAACAGAAGTCATATTCAAGCATTTATG14.0No Hit
GCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGG14.0No Hit
ACCTCCCCGTGTCAGGATTGGGTAAATTGCGCGCCTGCTGCCTTCCTTGG14.0No Hit
CGTGTGCTCTTCCGATCTACCCCGGGAGACGTGTGCTCTTCCGATCTACC14.0Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTTCCTATCTGGCCACGGAGACGTGTTATCTTCAAATCTGTCCACCGA14.0No Hit
GAACACCCCAATAGACCCTACTAGATAACCTAACCACTCATAATCAAAAA14.0No Hit
CACATAAGCTGCCAGCGCACTCCTATATCCAACATGCGCTCTAGGCTGCT14.0No Hit
CTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTAT14.0No Hit
GTTACACACTCCTAACCGGATAACGCCTTCCAACACCACCGACCAGCTGT14.0No Hit
GTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAAT14.0No Hit
CGGAGGGTATATGCCACTTCGCTGTACAACAAAAGCTTGCTAAAACAACT14.0No Hit
TGCAAATCGTTCGTCTGACTTGGGTATAAGGGCGAAAGACTAATCGAACC14.0No Hit
GAGCTTGGATACGGTTTCCCGATCGGAGATCCATGGATCACAGACGGTAT14.0No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGCCAGGAGAGATGTT14.0No Hit
TCCCTCAGGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAA14.0No Hit
GTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTACCCGATA14.0No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC14.0No Hit
CGCTGAAGCCGAGCCACGCCGTTAGGCGCGTGCTGCAGACCACGATCACC14.0No Hit
GCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAAC14.0No Hit
GAAGAGGGGCGACCCCAGGTCAGGCGGGATTACGCGGTGAGTTGAAGCAG14.0No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)