FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7E2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7E2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAAGGGAGTCCTTTTCTACGGTCATCCTGGATGTGGGAAAACCATTT15.88235294117647No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT15.88235294117647No Hit
CTACTCTTAGCTTCTCCATTTTCATGTTATGCTCAAACCACACCATTGTT15.88235294117647No Hit
TGATTCAACAGATATTATAAATGGAAATTGTAATTGTAATAAAAAATTTT15.88235294117647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15.88235294117647No Hit
GTCTGTTCACTGGAGGTGAATTTTGATTTTTTTGGCCGATCGGATTTGAG15.88235294117647No Hit
GGTGAGTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGT15.88235294117647No Hit
CTTCCGATCTTTAGACGTGTGCTCTTCCGATCTTTACACGTATTCTCTTC15.88235294117647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATCCCCA15.88235294117647No Hit
TTCTGTGACTGGAGACTGTTCTTATGATGATACTTCAGCTAAAGCACGTT15.88235294117647No Hit
ACCCAAGTTTGACAAGTCCGTCATCGAAGCCCTTCTCGCAATGTACGGTA15.88235294117647No Hit
ATATATATTAATCCTTCTATGCTTCCATCAATATAATAGCAAATCAAATG15.88235294117647No Hit
CTATCTTATATTATTATATAAAAAAAATTTAGTTCTTCATTAAACAAAAC15.88235294117647No Hit
GGTTGAAACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTT15.88235294117647No Hit
CAATATGAACTCGAAGTTTCCTTCGTAACCCCCGGAACTTCTTCGTAGTG15.88235294117647No Hit
GCCCCCCGGCTGAGATCGGAAGAGCACACGTCTCCCAGGGGAGATCGGAA15.88235294117647Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTACCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGGTGAGG15.88235294117647No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)