FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7D9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7D9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT210.0No Hit
AAATTCAACCGTAAAGAGGCAGAGGTTTTCTGCGTTGAAGCAGCTTCAAA15.0No Hit
CTCCTTAGCGGATTTCGACTTCCATGACAACAGTCCTGATGTCTTAATCG15.0No Hit
AACTACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAA15.0No Hit
CCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGT15.0No Hit
CCCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGC15.0No Hit
CTCCTGGTCTTAATTGGCCGGGTCGTGCCGCCGGCGCGGTTTATTTTAAT15.0No Hit
GCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCGTTAGG15.0No Hit
ATACCCGGCCGTCGGGGCAAGAGCCACGCCTCCATGAGTAGGCGGGCGCG15.0No Hit
CCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAAT15.0No Hit
CGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTTAGGTGGATCC15.0No Hit
GTGCTCTTCCGATCTGTTATGGGAGACGTGTGCTCTTCCGATCGGGTATT15.0Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCCGATCCGAACTAAGGCCGGCTTTCAGCGATTCGCTCCGCCTCCCAGT15.0No Hit
GTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGAA15.0No Hit
TTTTTGGAATGTATTTCAACCCCCTCCCACTGTCGTCCCCCGAGACAATC15.0No Hit
CTTCAGGATCCTAAGAACAAGAATGAGGAGATAAGAGATCAGAAGCTCAA15.0No Hit
GAGTTTCCTCTGGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGG15.0No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT15.0No Hit
GAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTC15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)