FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC22.5316455696202533No Hit
GAACGGCTCTCTCGGGAGCTTTCCCCGGGCGTCGAACAGTCAGCTCAGAA11.2658227848101267No Hit
GTGCAACAAACCCCGACTTATGGAAGGGACGCATTTATTAGATAAAAGGT11.2658227848101267No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11.2658227848101267No Hit
GGCATAATCTGCCATAGTTAGTGTTGATGGAGCCACAGCAGTAGCAGGAA11.2658227848101267No Hit
TTCAAGCACCAATGCTGTATCACAAAGCCGAAGAACATAAAATACAATGA11.2658227848101267No Hit
AGAAAGATCAATTAAAAAATATAAAATTAATTTAAAGAGATAACCCACCT11.2658227848101267No Hit
CTCCGCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGACAGTG11.2658227848101267No Hit
GCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATC11.2658227848101267No Hit
GATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGC11.2658227848101267No Hit
GTTTATAAGTACATGCAACAATGCTGCAGTCTCCAGGGTGTCCAATGAAT11.2658227848101267No Hit
CCTATGATGTTATCTCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA11.2658227848101267No Hit
CCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAAC11.2658227848101267No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG11.2658227848101267No Hit
CCAGAGAGGGTGAGATCCCCGTCGTGCACGCCCCCTGTAGCCCAACGAGG11.2658227848101267No Hit
CCCCTCGACCAGCCTTAGTAGTACGATGCAGCACATGCTTTGATCCCATC11.2658227848101267No Hit
CATTCACAGAGCATCTCTTAACATTGGTCATATCAACATTGTTCTCTTCC11.2658227848101267No Hit
GGATAGAAAAAAAGTCTAAAGCAAATAATCCTTAAGTTTATTTAAATCTA11.2658227848101267No Hit
GAACGGACCGTACTGGGCCGGCAGGTTGACGTTTTCCAGATCTTGGTAGG11.2658227848101267No Hit
GTCCTAAGATGAGCTCAACGAGAACAGCAATCTCGTGTGGAACAAAAGGG11.2658227848101267No Hit
AAACAAAGTCAATTTGTTTTTGTTAAAGCCCTCCTCATGACTCATTCCTC11.2658227848101267No Hit
TTAGTGGGTAGTGTGCTGTTTGTTGAATTGCCTGTGGATGGATTTGTGGT11.2658227848101267No Hit
CTCTACCGATCTGCCCTGGGAGGAACACCAGACCCAAGAAAAATATCTCA11.2658227848101267No Hit
GCAATAACTCGCATGTCGCATCCCACCCTTCAAACCCCCATTCATAATTT11.2658227848101267No Hit
GTCTTACCCTTTGTTTGAAGACACCAAATGATGACAAGATGGTAGAGTGA11.2658227848101267No Hit
CCACACGGGGAATGCATTGAATGGAACCACGGGAATTGAGAAGGAAGAAC11.2658227848101267No Hit
AAACAAGGTCTACAAAAATCAGAACAAGAATAACAACCCGTTGAACATCA11.2658227848101267No Hit
GAATATGTCAGAAGCGGCTGATGAGGACCAGGATCTGGCTTTAGCTCTGC11.2658227848101267No Hit
GGTGCCATCGGAGTCCTATAAGAAACATCAGCTGCTCCCTGTTCGGAATA11.2658227848101267No Hit
TAAATTACTCGTACAAGCTAAACTACTCAATTTTGCAGAAACAAATGAAA11.2658227848101267No Hit
GGAACAAGAAGAAGACAATTTCTTGGACAAGAAAATGAAAGAAAACCAAT11.2658227848101267No Hit
TCGAATTGTAGTCTGGAGAAGCGTCCTCATCGACGGACCGGGCCTAAGTT11.2658227848101267No Hit
CCTTATTGTCATTCTTCGTGAGAGAAGCTCGTCTCTTATTAACGAGGCTC11.2658227848101267No Hit
CTAGCTCCCACCTCAGCCGCCTTCTTCCGTATCAACGTCGGAGACATATC11.2658227848101267No Hit
GTGCTACACCGATCGCTCATGGAGTTGATCAACAAGCGTCGTGGGCGTGG11.2658227848101267No Hit
GATCTAATCAGGGAGACGTGTGCTCTTCCGATCTACCCTGGGTCTGTCTC11.2658227848101267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGA11.2658227848101267No Hit
TACGTGTGATCTTCCGAACACCAGGGGATACTTGGGGTCTTCCCATCAAC11.2658227848101267No Hit
ATCTAGACGTGTGCTCTTCCGATCAAGACGTGTGCTCATAAGATATAGAC11.2658227848101267Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGCTAGTCTTGGTTCTTTATCAAAGGCTTTGAACTTTGAGCGCGGTTCT11.2658227848101267No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGCCGGTATCTGATCGTC11.2658227848101267No Hit
CTAAACTGGCTGTCACTAATATCATTGATCAAACCATTTCCTAACACTTA11.2658227848101267No Hit
CGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTG11.2658227848101267No Hit
CTGTTATAGTTAGGAGACAAATAAAGAGAATTAGAGAACAGAAGACCAGA11.2658227848101267No Hit
ACGCAGGTGTCCTTAGATGAGTTCAACGACAACAGAAATCACGTGTGGAA11.2658227848101267No Hit
AGCACACCTCTCCCAGTCCAGAACGGAACAGCACACCTCTCCCAGTCCAG11.2658227848101267No Hit
CCATAAAAACATTCGTATATTATTCCTTTGTGTTGTGATACTCTAACGAA11.2658227848101267No Hit
GGGTCTTCTTCTTCTACATAAAATTGATTCGCCTCGAAATTTACACAGAC11.2658227848101267No Hit
GTGTCCACCTTGCCGTTTCATTGCACCAGTGTTTGCTGACATGGCTAAGA11.2658227848101267No Hit
AATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTT11.2658227848101267No Hit
GACTAAGTCCCACTAAGTACAGAAAATGGCAGCAAAAGCTGAGAAGATCC11.2658227848101267No Hit
GATCATTACAAGTTCTGAAGAAAGAGAGACATGGCCCGATGTCTGAGACG11.2658227848101267No Hit
ATCCTATAAATCTGTCCTAAGAAGAGTAGTCTAAGCCTTCGTAATCTAAT11.2658227848101267No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGTGCACCGTAACCCGGGTT11.2658227848101267No Hit
AAAAAATACGAAAGCCCCCGACTGTCACTCTTAATAAATACTCCGATCCA11.2658227848101267No Hit
GTGTACAAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTT11.2658227848101267No Hit
CTTCTATACGGACATAATATAATACTGTGGAACAGTATCTTTCTTTTATC11.2658227848101267No Hit
TATATAAGTAGATGCAGTAAAAAAGCTCGTATTTGAACCTTGGGATCGGT11.2658227848101267No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAAACCTTACTATGTCTG11.2658227848101267No Hit
CTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGA11.2658227848101267No Hit
AGCAAACACACAAACGAAGATGCTTATGCCGTTTGTGTAAACACAATACA11.2658227848101267No Hit
GAATTATTTCTTGATGTAATTCATTTGTCTTTTATATGTAGTAATAAAAC11.2658227848101267No Hit
CAACCCACACCGACCCCCCGCCAGCACGCTCCCCCTCCGTCAAGACCACG11.2658227848101267No Hit
ATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGA11.2658227848101267No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA11.2658227848101267No Hit
CTCTAAGAAGAAGAAGTCTGAAAACCCTATCAAGGCCTCCCCGGCTCCTT11.2658227848101267No Hit
AAAAAAACAGTGGTGAAAAGCAAAATAAAACATACAAAAAACAATTAAAA11.2658227848101267No Hit
GTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCC11.2658227848101267No Hit
GAGATGCACCACGACACTATTCTAGAGGCACTTCCCGGAGACAATGATGG11.2658227848101267No Hit
AGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGTGCGTCACTAATT11.2658227848101267No Hit
GGATAACATCATAGTATTTCGATCCTAATCTGTTGCCATACGCGAAACAA11.2658227848101267No Hit
CTCTGGGAGATCTCAGTTGGTGATTTATAATTTTTTAAGCTTTTTTCTTC11.2658227848101267No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAG11.2658227848101267No Hit
ACCTATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAG11.2658227848101267No Hit
AGAGAGGAGCTTTTATATCTTGTCTTGAAGCAACGACTTCAAAAGAAATT11.2658227848101267No Hit
GAAAAGCACAAAGAACACAGTAGAAATCACTTGAACTCCACTTCCTCTCT11.2658227848101267No Hit
CCGTAGTAAATGGGGAGATGGTCTATCGAGCTGAGCCAATATGCAGTTTT11.2658227848101267No Hit
TCTTTATGTTCTTCCTTCCAAATGAAATCATCAGTTTCTATGAGTCGATG11.2658227848101267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG50.0145.05
CGGGGGC50.0145.06
TCGGCGG50.0145.02
CGGCGGG50.0145.03
GGGGGCC50.0145.07
GGGGCCT50.0145.08
ATCCGGA50.0145.0145
GGCGGGG50.0145.04
GTCGGCG50.0145.01
GGGCCTC50.0145.09
Produced by FastQC (version 0.11.5)