FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGT11.9230769230769231No Hit
ATATAAGTACACATGACATAACAAAATGGTAAAATATACAAGAAAAGTTA11.9230769230769231No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTAATCATGGCGTACATACAACCCCC11.9230769230769231No Hit
ATCAAACACAAATGATCTTTGGCTAGAAATAAAGCAACGCTAAAGGTATG11.9230769230769231No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC11.9230769230769231No Hit
CCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCAT11.9230769230769231No Hit
GGTTTGCACCTTGTAGCGGCCACCCGTGCGCTTTTCCACTTCCTTGACAA11.9230769230769231No Hit
TATCTCATCTCCAAAAAACAACAAAACAAAAAAAACAAAACAAAGCCATT11.9230769230769231No Hit
TTTGTGTGAAGTGTGTAAGGTTCTCCACTTGAATTCCCTAAAAACTCGAA11.9230769230769231No Hit
GGTCCCTGCGGATGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATG11.9230769230769231No Hit
TTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCA11.9230769230769231No Hit
GTAAAACAGGAGCCCGAGATCGATTGTGGAACCAGCTAAGTATGCGAAAA11.9230769230769231No Hit
ATATGGAGTTTGTCACAAGTCCAAGAAAAGTTTTTAGGCATATAACTTGC11.9230769230769231No Hit
TCAAAGTATCAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCG11.9230769230769231No Hit
CCGTTAGGCGCGTGCTGCAGACCACCATCCCGGCAGCGACGTCTAAACAA11.9230769230769231No Hit
AGAAAGGTCGCTTCAGCTCTCAAAAATTTCACTCAACGCACCTTCAACGT11.9230769230769231No Hit
CTTGAGATTTGGATGAGAATGTTGAGAGTAGTATAAAGGTCCATCAACCT11.9230769230769231No Hit
AAAAAAGCATATCAAGATTCATTTGCCAATATTAGGAAAGGGGGTTCTTG11.9230769230769231No Hit
GCCGCAGCCCCTCCCTTTATGCCATATCGTTTAATTGCTATGTATTAGCT11.9230769230769231No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTG11.9230769230769231No Hit
TTCCATAAGTCGGGGTTTGTTGCACGTATTAGCTCTAGAATTACTACGGT11.9230769230769231No Hit
CTTAAGCGCCATCCATTATCGCGGCAAGTTGATTCGGCAGGTGAGTTTAT11.9230769230769231No Hit
GAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAA11.9230769230769231No Hit
ATTCATGATAACACGACGGATCGCATGGCCTCTGTGCTGTCGACGCATCA11.9230769230769231No Hit
GTGAAAGCGTGGCCTATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGA11.9230769230769231No Hit
CTTGGAAGTAATTAACAGCACGATTGTGGCTGCGTTCAAAAAAAAACCGA11.9230769230769231No Hit
CAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC11.9230769230769231No Hit
GATATGAATACGAGTGTGTACGTGGACGAAGAAATGTGTTACGAAGATTT11.9230769230769231No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAA11.9230769230769231No Hit
GTTTGTGACCTTCTAAAGAAGCTAGCCTTTCCAAAACCCTATCTCTCTTT11.9230769230769231No Hit
CAACTAATCAGAAATCAAAGTATTTAGGTTCAGGGGTGAGTATGTTCTCA11.9230769230769231No Hit
TTCGATGGTAGGATAGTGGCCTACCATGGTGGTAACGGGTGACGGAGAAT11.9230769230769231No Hit
GCTCAGATGTGTAAGGGAGGACAAAATTGACGGTGAGCTTCGTCGGGATA11.9230769230769231No Hit
GCTCGACGGGTGGGTCTTGCGGTCAAGCTCCCTTCTGCCTTTGCACTCGA11.9230769230769231No Hit
ACCTTGTAATAGCTCCATTCGGTTTGATCACCGTTTTTATAGGAGAGAGG11.9230769230769231No Hit
AGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAAACTTTGATTT11.9230769230769231No Hit
CAAAAGGGTAAAAGCTCGTTTGATTCTGATTTTCAGTACGAATACGAACC11.9230769230769231No Hit
GAGCACTGGGCAGAAATCACATTGCGTTAGCATCCGCAGTGACCATCGCA11.9230769230769231No Hit
GCATTGGTCGGCTTGTCCCTTAGGTCGCAGATACGCTCCTGCACTTAAAT11.9230769230769231No Hit
GGATAACATCATAGGATATCGATCCAATTGTCATGTCCTTCGGGAACGGA11.9230769230769231No Hit
ACATTACATAAAAAGGTCCAAGTACATTATTTGACTGATATAAGAGAGAG11.9230769230769231No Hit
GAACTGGGACCCCCGAGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGT11.9230769230769231No Hit
GCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCGCCGACCGA11.9230769230769231No Hit
GGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAAC11.9230769230769231No Hit
GGAAAGGGGCGCCACAGAGGGTGAGAGCCCCGTCTTGACCGGCACCTCTC11.9230769230769231No Hit
CCCGCGAGGGATCCCGCCAATCAGCTTCCTTGTGCCTTACGGGTTTACTC11.9230769230769231No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG11.9230769230769231No Hit
CTCTAGAGGTTTGACAAGGAAGCCAATGGCTCTGATTAAGAAGCTGAGGA11.9230769230769231No Hit
ACATAACTGAACCGGGAAATCGCATACTAAACCAATCAAAATCAGACTAA11.9230769230769231No Hit
ATTCAAATGAGAACTTTGAAGGCCGAATAGGGGAAAGGTTCCATGTGAAC11.9230769230769231No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT11.9230769230769231No Hit
CACCAAAGGCGGACCGGCCGACCCATCCCAAGGTTCAACTACGAGCTTTT11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGCA50.0145.03
CAGATAA50.0145.0145
AGCATAT50.0145.06
AAAGCAT50.0145.04
AAAAAGC50.0145.02
AAAAAAG50.0145.01
ATATCAA50.0145.09
CATATCA50.0145.08
GCATATC50.0145.07
AAGCATA50.0145.05
AAAAAAA202.6174588E-429.0130-134
Produced by FastQC (version 0.11.5)