FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATTATTATCCGGTTGTTGCGGAAAGCTGCCTAAAAGGATGCAAGG11.7857142857142856No Hit
ATTTCGATCCTATTGTGGTGGCCTTCGGGATCGGAGTAATGATTAACAGC11.7857142857142856No Hit
CCGTGGAACCGGGTCGCGGGGTGCCCGGCCCCGTCCTCCCGCCGCGACCC11.7857142857142856No Hit
CCTGAGATCCGATGCGAACAATCAGTGGAAGGAGTAGTCAATCGAACTCA11.7857142857142856No Hit
ACGTGGAGGCATCCCTTCACGGCCGGCTTCTTAGAGGGACTATGGACGTT11.7857142857142856No Hit
AACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCA11.7857142857142856No Hit
GAAATGGCTCACATCATCTTCATCGGGGTATGGCGGAGAAGTTTCAAGTG11.7857142857142856No Hit
ACACCGGGATATAAGCTGCCTGTGGTTCTACGACCCCGAGACCAGGCAGT11.7857142857142856No Hit
CTCTTTATGTTGGACTATTGATTATTTTTGAAGATAAGTTTCCTTTGGAT11.7857142857142856No Hit
GCCATTAAGCGCGAGACCTAAACAAGGCCGAATCCGCAACAGCCCTGCCT11.7857142857142856No Hit
TTTGGATGCTGCAGCCATTGCTGGCCTGAGGCCATTGAGATTGATGCATG11.7857142857142856No Hit
GGATACATTAGCATGGGATAACATCATAGGATGTCGATCCTATTGTGTTG11.7857142857142856No Hit
ATTCATTAAGGATTTCACAGTTGATATTGAAGGTGTAGGCCATGTTTGCA11.7857142857142856No Hit
ATAAACAGGATTGAACATTAATAAATCTTAAATGCTATTAATGTAATTCG11.7857142857142856No Hit
GGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCC11.7857142857142856No Hit
CCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACG11.7857142857142856No Hit
CCATAGAGAAAACCAAACAAGCAAACCACAATCCCAGTATATCCAAGTCC11.7857142857142856No Hit
ATGTACCAGCTGATGATTTGACTGATCCTGCTCCTGCTACAACTTATGCT11.7857142857142856No Hit
CTCCAAGAGAGAGGCATTGTTTTGTAGTCAAAGAAACTAACTAACTTTCG11.7857142857142856No Hit
AACCGTGGGAGGGGTCGTCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTT11.7857142857142856No Hit
GGCGGTTGCTTTTAGAGATATGGTGCTTGCTGTTAGCACTAGAGGAACTC11.7857142857142856No Hit
CCTCGACACGGACGGTGACAAGCCGGTACCAGAAAATCAACTGGTTACCC11.7857142857142856No Hit
AAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAAGC11.7857142857142856No Hit
GTTTAGACTAGGACGGTATCTGATCGTCTTCCAGCAACCAACTTTCGATC11.7857142857142856No Hit
GCGTCATACATCAACATCATAAAGGACACCTTTAGCGCTGCGTTGGGCGA11.7857142857142856No Hit
GTACAGAGAGGGTGCGTAGTAAGAGAGACCGACCAGTTGAGTTTGAGAAG11.7857142857142856No Hit
ACCTATCACCTTACGTCACCATTCATGACCCCTCACACCCAATCGAGAGC11.7857142857142856No Hit
CAACAAAGCAGCCGACACGTCCTACCTATTTAAAGTTTGAGAATAGGTCG11.7857142857142856No Hit
TTTTAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCCCACGGAAT11.7857142857142856No Hit
GAATTGAAGAAATTGAATCACCAAAGCAGAAGATCAACACCGCGATCAAC11.7857142857142856No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA11.7857142857142856No Hit
CTTCTGAAGCTGTTGATATTCACAAGCTTATGTCAACAACCTTCCTCGTT11.7857142857142856No Hit
GTTACAAGCCGAGGCCAAAGCAGCTGAGGAGGCTCGGAGAAAAGCCAAAG11.7857142857142856No Hit
CATAAATATAGTATTAATTATGAAACTAATACACACAACACATAGAAGTC11.7857142857142856No Hit
CAAAAAGCTGCTCGACAGCATATGATGTTCGCCCCTGCACCTGTAGCCCT11.7857142857142856No Hit
TCACTACCCAATGACGCTAATCTCCCTGCAAATGAGCCAGAGATGGTGGA11.7857142857142856No Hit
CTTCCGAGCTGGAACGGCGAGGTAGTGACAATAAATAACTATACCGGGCT11.7857142857142856No Hit
TACATTACCTTTACTCCAAAAAACCTCTCATCTGAAAAATAACACATATG11.7857142857142856No Hit
GAAGAAGAAGGAGAAAAAGAAGAAGATTGTTGAAGGAGATCATGTGAAAA11.7857142857142856No Hit
CTCATAATCCAGCGCACGGTAGATTCGAGCAACTGGCTATACAACCAAGC11.7857142857142856No Hit
CCTGTGGGCTCCCCATTCGACCCTTCTAGAAACACGGAAAAAGGACTCTG11.7857142857142856No Hit
GGTTTAGTGGACTTCTCGCGACGTCGCGGGCGGCGAACCACCCACGTCGC11.7857142857142856No Hit
CAAGAGAGGGTTCTCAGAGACGGTTGATCTGAAGCTAAATCTGAATAATG11.7857142857142856No Hit
CCGGTGGCACCTTATGAGAAATCATAGCTTTTGGGAAAATGTGGTACTAA11.7857142857142856No Hit
AGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCG11.7857142857142856No Hit
GTGAAGCAGGGATACACTACGACAACCTGCCCCCAACAAACGTTGATGTA11.7857142857142856No Hit
ATCTCCAAGAGGAGTCCCTCAGATCGAAGTGACATTTGACATTGATGCCA11.7857142857142856No Hit
GACAAGAAGGATTGGTGAGGTCTAAGAAACAAGAAATGGAGATTCAAGAA11.7857142857142856No Hit
GGGGAGGGAGCGTACGGTAAGCACATGGCGTATGTTAGCACACTAGTTCC11.7857142857142856No Hit
ATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGA11.7857142857142856No Hit
TCTCGATTCCGTGTGACGGCTCAACTAAGCAGCCGACTCGTCCTACCTAT11.7857142857142856No Hit
TGTAAAAAGGGACAAGAGACAAGTGGCCCTTAATTGCGCCTCCGCGGAGC11.7857142857142856No Hit
TATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCT11.7857142857142856No Hit
GTAAAAGATCAGCAGCAGGTAAGAACTCAACTTGATGAGTAACATATATA11.7857142857142856No Hit
TCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTAT11.7857142857142856No Hit
CTCCAGATTACAATTGATAGTCTCTTTCCTTTTGTCGTATATCTAGAGTC11.7857142857142856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTG50.0145.08
GGATTGG50.0145.09
GACAAGA50.0145.01
AAGAAGG50.0145.04
AAGGATT50.0145.07
GAGACTC50.0145.0145
GAAGGAT50.0145.06
CAAGAAG100.072.53
AGAAGGA100.072.55
ACAAGAA100.072.52
Produced by FastQC (version 0.11.5)