FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGAGATCGGAAGAGCACACGTCTCCCTGGTGAGATCGGAAGAG12.7777777777777777Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTTTCGAGTCAGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAG12.7777777777777777No Hit
GCTTGAGGAAGAAGATGAAGCCCGAGGAAATGCTAACGGGGACTCATCAT12.7777777777777777No Hit
GGGGCAATCCGGTCCGCCGATCGATTCGGGGCGAGGACCGACGCGCATGA12.7777777777777777No Hit
AGCCAATGATTAGAGCCAATGGGGGCGCAAAGCCCTAGACCTATTCACAA12.7777777777777777No Hit
TTGTTGAATAAAGACGTGACCGTTGTGTTGCGTATCAACTCTCTTAAGCT12.7777777777777777No Hit
GAATACAACCCTGAAGCTCACGTGCGTCCATGAAGAGGTACACTACAAAG12.7777777777777777No Hit
CCAATTGTGATCGACTTCGGCATCTGACTAATCAATAACAGCGAAAGTCG12.7777777777777777No Hit
CATGAGAGCTATCTCCTTCCATGTGACCAATGAAGATAAAAAACGACAAA12.7777777777777777No Hit
GATTCAGACTTAGAGGCGTTCAGTCATAATCCAGCGCACGGTAGCTTCGC12.7777777777777777No Hit
GCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGT12.7777777777777777No Hit
ATATAGAACCTAACACAATGAAATGTGGAGAATGGTAAAGAACAGAAAAC12.7777777777777777No Hit
GAGCAAATACTGAATTGATGTGAGCACTGAAGCAACGTCGAAGATGAGCC12.7777777777777777No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAG12.7777777777777777No Hit
GGTAAAGAGGCGGTACTACGGTGCGAAAACGAAGGTGATAGTGAAGGAAA12.7777777777777777No Hit
CGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCA12.7777777777777777No Hit
CAATCAACGACATATGAACACGGTTTTCGTGTTGGCTTTAAAGGGAGTTA12.7777777777777777No Hit
GGGTGAGGACTAATCAGAGGTAAGCATGCCAAACAGAAATACAAGTTCTC12.7777777777777777No Hit
GAACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC12.7777777777777777No Hit
GGCGCCCACAGGCACCTCGCCCGCGCTGGCTGCGGCTTGCGCCTCGGCCA12.7777777777777777No Hit
CCTCAAAGCAAGCCTACGCTCTGCATACATTAGCATGGGATAACAACATA12.7777777777777777No Hit
CTGATGCCGAGGCACGCCGTTAGGCGCGTGCTACAGACCACGATCACGGC12.7777777777777777No Hit
CAACATAGGATTTCGATCCTATTGAGTTGGCCTTCGGGATCGGAGTAATC12.7777777777777777No Hit
GTCTACAAGTTTCTGAAATCAAGCAAAGGCGGGCTCTTTGGAGACGGCAT12.7777777777777777No Hit
ATACAAATAAGTCAGAAAAACTATAGAGACACAATACAACACGGTCTGGT12.7777777777777777No Hit
CTTCATAATACTGCTAAGTCGTTTAAGACTGAAGGCAAGGTTACTCCTGA12.7777777777777777No Hit
GCTATGGGCCGAGGAGGACTTTTAGCGAGTTTGAAGATGAGTTGACTCGG12.7777777777777777No Hit
AAAGACAAGAGAGCGACTAAAAGCGACAGTAAACCCGTAAGCCGCAACTA12.7777777777777777No Hit
CCCTCTAGGGTTTCTTTGATCAAACCAGAGAGCCGTCAGGGAGAGCAAAA12.7777777777777777No Hit
CTGGAGAATGGTGCGGAAGAGATGAAGAGAGGTGTTAAAGCGGATAAGCA12.7777777777777777No Hit
TATCTACTCACACACCACTCTACATAACCACCACCACCACAACCACATGT12.7777777777777777No Hit
CTCATAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGA12.7777777777777777No Hit
TACAAGAGGTTATCGAGTATATTCAATTCTTACAAGAGAAAGCAGACAAG12.7777777777777777No Hit
AATGAAACAACAGAGCAACGGCCAGAGCCAGCCTCGACATATTATCTAAT12.7777777777777777No Hit
CATTAGAACAGAGAGCTATGAGTGTATTGAGAATTGCAAATCTGCAAATG12.7777777777777777No Hit
CCTAATAGTAAGAAGCACAAAAACAGTTGCAATAATCTAAAGCGTTGAAA12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)