FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGAGCATGCCTGTCGGGCCCCGAATAGATGGTGAACTATCCCTGAG12.4390243902439024No Hit
CATTAAGAGAGTCATAGTTACTCCCGCCGATTACAACCGCTTGGATGAAT12.4390243902439024No Hit
GACCAACAAGGGGTAGTACAGCATTTTTCACATGTTGTCCAGCGACTACG12.4390243902439024No Hit
CCAGTAGCTTGTTAACATTATCACTTGCATAGCGCACGATTTCAATTAAA12.4390243902439024No Hit
CACCAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTAAGT12.4390243902439024No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGCGCCCGGGC12.4390243902439024No Hit
TTTAAAACCTTCCCCTAGTTTTCTCCATCTTATTCCGAGAATTAGCAAAC12.4390243902439024No Hit
GATCTGGCTGGGGACAGGTAATAGGGTAATTCATTTTCCAAGCCCAGCTC12.4390243902439024No Hit
CAAAGGGTCTAGGTCGATTAAGACAGCAGGACGGTGGTCATTGAAGTCGA12.4390243902439024No Hit
CGCTGTGCGTGCTGGCCCTGGCGGGCGTATGGATGGACTACCGCTCGGCC12.4390243902439024No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12.4390243902439024No Hit
CTCCCCAGGTCAGATCGGAAGAGCACACGTCTCCCTGTGCAGATCGGAAG12.4390243902439024Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCATCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCG12.4390243902439024No Hit
AGGCAGATCGGAAGAGCACACGTCTCCCATGGCAGATATGAAGATAACAC12.4390243902439024Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTATACCGAGAGAAGAGACTTGAATTGCTCAGTTTCTTTAAGTCCTTTTA12.4390243902439024No Hit
TTCTGCCATGAGGAACTCTTGGCACAGGGGACGTTCCAACAGCCTGATGA12.4390243902439024No Hit
GCACACGTCTCCCCAGTGTGGGGAGACTGTCTCTTATACACATCTCCGAG12.4390243902439024No Hit
CCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAG12.4390243902439024No Hit
ATCCGGGACCGCGTGGACTGGTCCAGCGTCGTCACCAAAACGGTGAAAGA12.4390243902439024No Hit
TCTCCAGGGAAGATCGGAAGAGCACACGTCTCCAGGGAAGATCGGAAGAG12.4390243902439024Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGGTATCTCGAGCTTGTAATGCTTTGGCTATGATTCGTCCGACTTCTTT12.4390243902439024No Hit
AATATGCTGTCACAAACCTTTTCAGTAGATTTGGTGGAAAGTAGGGGTAC12.4390243902439024No Hit
ACCCCACACTCTAAATTACATAATATAAACCTTTACACATCAAATCCATT12.4390243902439024No Hit
GCAACAGCGTGCTCCGGGCGTCGGCGTGAGGGCACCCAAATAGCCGCGGG12.4390243902439024No Hit
AGGAAATTAAGACATTTTAGCACTCACAATGAAAGCAAGAGACCATAGGA12.4390243902439024No Hit
CTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCC12.4390243902439024No Hit
CTCCGAATGTATCCAGAGCGTAGGCTTGCATAGAGCACTCTAATTTCTTC12.4390243902439024No Hit
TCTATACTGATCCAACGCACAAAAAGGTAAAAGAAAACTGAAGGCAAAGA12.4390243902439024No Hit
TCTCTACAGGGGACAAAAATCCTTCTTACATTTCTACAAACAAAACAAAA12.4390243902439024No Hit
CGTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCG12.4390243902439024No Hit
AAGTAGATAAGACTTATCATACAAAAAAAAAAAAAAAAAAAAATAAAAAA12.4390243902439024No Hit
CTCCCAAGCGAGATCGGAAGAGCACACGTCTCCCAAGCGAGATCGGAAGA12.4390243902439024Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTCCATACAACTGTCCTAGACTCGCATTCTGCTTGATGCTTTTGCGAGC12.4390243902439024No Hit
GTCTTACGGCAATAGTGATAATCAACGATTTCAACATACCTAGCGCTCGA12.4390243902439024No Hit
CTCGAACACTTCTCAGAAGAACAAGGTCGGTCGGCGGTGCACCAGCGAGG12.4390243902439024No Hit
GAGTGGGCAGCGTGGGTCCTCGAATAGTTCGACAACTTTAGCATCCTTTC12.4390243902439024No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCCGCCCCGGTTA12.4390243902439024No Hit
GAATAATATCACCGATTTAGTAGATGTTCTCTGCCTTGAGGCATTTGTAA12.4390243902439024No Hit
GATGGGTAGAGCACTCGTCTTCTGTTCGGAAGTGCTCAGGTCTACAGATC12.4390243902439024No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCACCAA12.4390243902439024No Hit
GTGAAAGCGTGGCCTATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGA12.4390243902439024No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers