FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGTATCGCCGACC23.571428571428571No Hit
CTTCAGGGGCGACCCCAGGTCAGGCGGGATTACCCGGTGAGTTTAAGCAT11.7857142857142856No Hit
GCTCCGGGCGTCGGCCTGTGGGCACCCCCATCGACCCGTCTGGTAACACG11.7857142857142856No Hit
ACAATATCCATAACCAGTCCACCCTTCATCTTGATCTCACCATCCGTTGT11.7857142857142856No Hit
GAACCAAATATTAATTTGAGAACTGTGAAGAACTAAGCTGTGAAATTGTC11.7857142857142856No Hit
CATTAAATCAGTTATAGTTTGTTTGATGGTAAATACTAATCTTATAAACG11.7857142857142856No Hit
CAAAAATGTTATCTCAGAGGATATACATCAGACCTCGAAGTAGCTCGTAA11.7857142857142856No Hit
GGATAAGAGAAGACGATCAGGGAGAGAGGATACTGAAAAAAGGAGAAGGA11.7857142857142856No Hit
GCCATAGTCCCTCTAAGAAGCCGGCCGTGAAGGGGTGCCTCCACGTAGCT11.7857142857142856No Hit
CTAACAAAACGCTAATCTCTGGGAGCACGACCAGACATAGCACCAGAAAG11.7857142857142856No Hit
AAACAGAACACGGTATCTTCTCACCACAGGACTAACGCAGCCGCCGTGAT11.7857142857142856No Hit
GTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGG11.7857142857142856No Hit
GTTTAATAGACATGATGATTCCAACGGAGCTGATTACATTCATAGCCCAT11.7857142857142856No Hit
TGCTAACGCAATGTGATTTCTGCCCAGTCCTCTGAATCTCAAAGTGAATA11.7857142857142856No Hit
GCCAAAAGCCTCGTCATCTATTTAGTGACGCGCATGAATGGATTAACCAG11.7857142857142856No Hit
ATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGTT11.7857142857142856No Hit
AACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAG11.7857142857142856No Hit
TGATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTG11.7857142857142856No Hit
CTATAAAAATTCTCTTTACAAGATAGAACTATAGGCCTATAAAAATCTAT11.7857142857142856No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTAGAGTCCGAGCTCCAGCTA11.7857142857142856No Hit
TAATAGAAAACGGATGAAGTAGAAGAAAAAATCACACTCAACATCTATAT11.7857142857142856No Hit
CTCCAGGTCTTGTCACCAAAAACCTTCCCCGATTGACAGAACGTTCCGCT11.7857142857142856No Hit
GTAAAGAACCGAATAAACCAGATACGGGTAATCGGAGAGCCGAGACACGA11.7857142857142856No Hit
TTTTTAACGTTTGACTGAGAATTATTTTTCTATTAAGTATATGTAGAATG11.7857142857142856No Hit
AAACACAGGTTCACCAAAAAACACGCCTTCGAAGATTTCAATGACATCTA11.7857142857142856No Hit
GCATTGGGAGACGTGTGCTCTTCCGATCTGCATTGGGAGACGTGTGCTCT11.7857142857142856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCAAAGAGCCCAACAAGATAAGCCTCAGCTGCTTCCTGAAGAGCTGC11.7857142857142856No Hit
GCCCCCGACTGTCCCTATTAATCATTACGGCGATTCCAAAAACCAACAAA11.7857142857142856No Hit
AATTACTATCGCGACGCGGGCATCAGTAGGGTAAAACTAACCTGTCTCAC11.7857142857142856No Hit
TTCCTGTCCCGTATGAGGTCCAGAGCATGACTTGGAGGAATGTGTTTTCC11.7857142857142856No Hit
CAAAAGCAGAATTCTCCTTAAAAGAACACAGACCTGCACTATTAATCTAC11.7857142857142856No Hit
GGTGGGAACTCGTTGACTACAGCAGGGTAGCGTTCGTGCTTCCCTGCACC11.7857142857142856No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCAGCG11.7857142857142856No Hit
GACCAAGGAGTCTGACATCTGTGAGAGTCAACGGGTGAGTAAACCCGTAA11.7857142857142856No Hit
ATCCTGACACGGGGAGGTAGTTACAATAAATAACAAAACCTGACTCTTAT11.7857142857142856No Hit
GGCATAGGCCGCCACCGTAATCCCCGACGCTCCACGCCACGAAACGAACG11.7857142857142856No Hit
CAAGTTTCCTCTGCAAACTCAACAAAGCTCTCTCAGCCTTTCGTCTCTGC11.7857142857142856No Hit
CCTCCAAACTCTATGCACGGAAGAGAAACTCTGGCAAGAACTCTCCATCG11.7857142857142856No Hit
AAGTAACTCGACTGAAAGAAGAGGGGCTTGCTGGTTTTTCCATCATCCAA11.7857142857142856No Hit
GTTCTTGTTGTTGTTCTTTAGATTGACGTTGTACCAATACTTCTTAATAG11.7857142857142856No Hit
GTTATATAGAAAATCTTCTCCCGAAACTCCACCGTGATCCTCGTTATGGA11.7857142857142856No Hit
CTAACAAGGATTCCCTTAGTAACGGCGAGCGCACCGGGAAGAGCCCAGCT11.7857142857142856No Hit
TGATAACTCGACGGATCGCATGGCCTCTGTGCTGGCGACGCATCATTCAA11.7857142857142856No Hit
GAGCACGCTGATGCCGAGGCCCGCCGTTAGGCGCGTGCTGCAGACCACGA11.7857142857142856No Hit
TCCTATTGATGGCTCCACGTTAATCTCTCCTCAGTTCCAACAGCTTGTCC11.7857142857142856No Hit
CCTTTGTCCTCATACTTGTCGTAGAGTACAGGAATGGTGAAGAGAAGGAC11.7857142857142856No Hit
ACCCAGATGAGAAGCTTCAAGAAGATGTGGTTACAACATTGTTGAACATA11.7857142857142856No Hit
AAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGCAAGA11.7857142857142856No Hit
ATCATAAGGTGCCAGCGGAGTCCTATAACCAACAACCGCTGCACACAGGT11.7857142857142856No Hit
AAAAAAAAATTAAAGGCGGTACGAAATACAATTAATCAAAAATTACTTAT11.7857142857142856No Hit
CGACGGGGCTCTCACCCTCTCTCCCGCCCCTTACCAGGGAACTTAGGAAC11.7857142857142856No Hit
ATTCAAGGGAGCTGAGAATCCAATGGCTTCTTAATCTTAAGAGCTACAAA11.7857142857142856No Hit
CAACCGGGCTGTAGAGGTCTATTCCTTTCCCTGAAGCTGGAGCAGATAGG11.7857142857142856No Hit
TCAAATGGGTGATAGTCTCCCTGCTTACTTGCAGAAACTTCACTTCTCCA11.7857142857142856No Hit
TACCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGTTT11.7857142857142856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACT50.0145.07
AAGTAAC50.0145.01
ACTCGAC50.0145.06
TCGACTG50.0145.08
CGCTCGC50.0145.0145
AACTCGA50.0145.05
TAACTCG50.0145.04
AGTAACT50.0145.02
GTAACTC50.0145.03
CGACTGA50.0145.09
Produced by FastQC (version 0.11.5)