FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCAAAACATAGCCTGCATAGAGGAGGAATGTATATTATTGAATGCTTA12.857142857142857No Hit
CGTGTGCTCTTCCGATCTAGATAGGGAGACGTGTGCTCTTCCGATCTAGA12.857142857142857Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCCACAACGGTGACTGGCCATCCACGGCGCAAGGCATCAATCGCCATCG12.857142857142857No Hit
TGTGAAACCTGGTAGCGGTTGAGTGTGAGGAGATGAGGGGCATGATTTTG12.857142857142857No Hit
TTCCAGAGACCGTCGTTGAGGTAATGCATGTTGTCAACTCCTATTCCTTT12.857142857142857No Hit
AAATACAACTATGATCGGATAGAGCATCTAAAAAAGGCGCCTGATCTTGG12.857142857142857No Hit
CTACCGATCTGCACGGCTTCCACAGCCATCATCTGGATGATCACGCCACG12.857142857142857No Hit
CATCCGCATCTTTCCCGACAAGCCGGTCACCAAGAAGCCCGCCGAAACCC12.857142857142857No Hit
CCCTTAAGAGATCTGTAGAGCAAACTGCACGCACCCACCATCTGCCCCGC12.857142857142857No Hit
CTGTGGATGTGAGCAAATTCTCTAGCCGACATGTTGTCAGTGCAGCATAT12.857142857142857No Hit
CCCAGTGCAGATCCCAAGATCACACGACTCCCAGTGCAAATACGAACAGA12.857142857142857No Hit
ATATATTGATGAGGAACGTTATTTGCAGGATTGTAGCTATTATAAAATTT12.857142857142857No Hit
TTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG12.857142857142857No Hit
AACCAAGCGCGATGACCAATTGTGCGAATCAACGGTTCCTCTCGTACTAG12.857142857142857No Hit
TCTCTGGCGCCCCTTTCAAGGGAACTTAGGCCCGGTCCGTCGCTGAGGAC12.857142857142857No Hit
TCTTAATTCTCGATTAGGGTTTCATAGATATCATCCGATTTGTTCAAATA12.857142857142857No Hit
CCTCCCACGGATTGAGAATGATCGCGCCCGTTGTCTGGAAATCAGCAACG12.857142857142857No Hit
AAAGATAACACTTCCCGAGGCCCCCGCCGAACTCTCCCGCCTCCCTAACG12.857142857142857No Hit
TACCTAAAGAGATCGGAAGAGCACACGTCTCACTAAAGAGATCGGAAAAC12.857142857142857Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GAGTAATGATTAACAGGGACAGTCGGGGGCTTTCGTATTTCATAGTCAGA12.857142857142857No Hit
CTTAGGTTGCTTCTGATTATTAACAAACTTTTCTCTTACGATTGAGTCGC12.857142857142857No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG12.857142857142857No Hit
GGGTAGGGTGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGT12.857142857142857No Hit
CCACTACTACATACCCGTTTTTATTTGATTAGAAGATCAGATTGCTTTTC12.857142857142857No Hit
ATGACCAATTGTGCGAATCAACGGTTCATCACGTACTAGGTTGAATTACT12.857142857142857No Hit
ACTAGAGATCGGAAGAGCACACGTCTCCCACTAGAGATCGGAAGAGCACA12.857142857142857Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTTTTTTTTATTTTTTTTTTTATTTAAAAAATCCAGCCCAAGCATCGT12.857142857142857No Hit
GCAAAAAGGTTAGAGATCATTACTAAAAACTCGGCAAGGAACTGCTTTAG12.857142857142857No Hit
GCTCTGTGAAGCTCGAAGTTGATCGCAACTACGCAGTGGCCAAAGAACTT12.857142857142857No Hit
CCGAGTGACGGCTCAACAAACCAGCCGACACCTCCTACCTAATTAAATGT12.857142857142857No Hit
CATAAAAGATTTATTGTTTCGTCAGCACCACCTGCATCAAAAAAGATAAA12.857142857142857No Hit
GTTATATCGGGTAAAGCCAATGATTAGAGGCATTGGGGGAGCAACGCCCT12.857142857142857No Hit
GGTCGGAACTAACACGACAAGGAATTACGCTACCTTAGGACCGTTATTGT12.857142857142857No Hit
TCAAGTTGCTTTCCGGCGAAGATCAATCTCTGCTGGTCCGGTGGGATACC12.857142857142857No Hit
GAACAGTGCATGGAACGGGTGAGCTGCCATGTCAGAGAAACTTGCTGGAG12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)