FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p7C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p7C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGGGTC11.5384615384615385No Hit
ATACGTCCACTGTTCGTGTTGGCAGAGTGCTGTGATTTTGGTAAAACAGT11.5384615384615385No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC11.5384615384615385No Hit
ACTTTTAACGTTATTTTACTTACTCCGTGAATCGGAGGCGGGGTACAACC11.5384615384615385No Hit
AGACAGTAGTGCTCTCGACGTTACCGGGAAATCTAGATCTGGATTCGAAT11.5384615384615385No Hit
GGCCAAGGGTGATGAACGAAATGTGAACCCTTGTCTCGCGTAGGTTGGAT11.5384615384615385No Hit
GTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGC11.5384615384615385No Hit
TAGTAGCAAATGGTCAAAGAAGGAGCAACCCGGTGTTGAGGTCGTTGGTC11.5384615384615385No Hit
ACAGGGTCCGGGCACGACGGGGCTCTCACCCACTCTGGCGCCCCTTTCCA11.5384615384615385No Hit
CCACTGAAGAAAGTAACGCTTCTAATAGTGACTAAATTAAGTACTTGGAT11.5384615384615385No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5384615384615385No Hit
GTATACACCGGATGAAATCATGAGTCTCGTTGAGTCTTCTTCACCGACAA11.5384615384615385No Hit
GTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTAACGCAAT11.5384615384615385No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGT11.5384615384615385No Hit
AAAAATAACAATCGCGGGCTGTTTCGAGTCTGCTATTTGGATTGTGTACA11.5384615384615385No Hit
TCGCTGGCGTGCCGCCCGATGACCTCGGGCCGCTGGGCCAGCGCTGGGGC11.5384615384615385No Hit
AAGAAAATGATCACAAAACAAAGCGAATCTCCCACTCCCACATCATTATT11.5384615384615385No Hit
TATTAAATAAGAAAAACTCCCAAATAAAAAACAAAGGACAAACAATTAAA11.5384615384615385No Hit
AAAAAAAAATATATACACAATCAACAAATGATAAAAATAATAGTAAGACT11.5384615384615385No Hit
GTTAGCGATGGGCGGCACCACGGTCTTGGAAATGCGTCAATAAGAGATGA11.5384615384615385No Hit
CTTCCGATCTGAACAGGGGGAACCCGATGCGGCGAATCGCGGCGATGTAG11.5384615384615385No Hit
CTCAATGACAATCAGAAACTTGATAATCACTATGGAGGAAGGCATGAACA11.5384615384615385No Hit
GTCATTTTTTATTCCTCACTCCGAGCCTGAATTATTGCTTTGCTCGACTT11.5384615384615385No Hit
TAGCAGGCCAGCGACCTTGTCCGTTCGAATGCCTGTTCTTTGCCTGAGCG11.5384615384615385No Hit
CCATCATGGACCGAATTGTCTGGAATAGGCTCACCTACTTCTTTTGCATC11.5384615384615385No Hit
ATCAGCAGCAACAAAAAAGGAAACATAGTAACGAAAACTCCATCAGCTAA11.5384615384615385No Hit
TCAAACGCCCGGTCGCTGGCTCTGCTGGGAAGTGGACTGATGAAAAGCTA11.5384615384615385No Hit
CTTCCGATCTTCCCTGGGTCGGCATCGTTTATGGTTGAGACTAGGACGGT11.5384615384615385No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTC11.5384615384615385No Hit
ACCATTATCTGTTTCACCATATTTGTCATTCAAGCATGGCAGCATTCAAC11.5384615384615385No Hit
GCTCACACCCCCGGGACACTACCACACATACCACAAACACAAGCAAACTC11.5384615384615385No Hit
GGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCCCATCCG11.5384615384615385No Hit
GACAGACATCAAGAACACAACTCCAACTACTGCCGCCCTAAAGAGTCCTC11.5384615384615385No Hit
CACCGGAGCTCAGGTCGCTCCGATCCTTAGGCTTGAGGAGGTTGCCGTCA11.5384615384615385No Hit
CTCCTGGTCTTAATTGGCCGGGTCGTGGGGGCGGCGCGGGTAGTTTGGAG11.5384615384615385No Hit
CCCCAACCGAGCCTGAGAACAACATGAAGGGTGGACTCATTCACGATATT11.5384615384615385No Hit
TTATAATACATCTATAAACATCACAAACAGAATACGATTCATATAGATAA11.5384615384615385No Hit
AAACATGGCCTAGTGCCACGTCACGGACGTCCACGTAGACATGAGTCAAG11.5384615384615385No Hit
CTAACTCGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGC11.5384615384615385No Hit
CTCCACCAGTAAATGCTCTTGACCTTAAGAAGCAATTCTAAGTCCGTAGT11.5384615384615385No Hit
GTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAA11.5384615384615385No Hit
CTCCATGATTATCCTTACACCTAACAGACAAAAGAACCTGTCCAATCGCA11.5384615384615385No Hit
CCATAATCATCTTCCACGTAATCTCTAACGTCTCGTTACGTTACGGTTTC11.5384615384615385No Hit
TAAACCAATACAGAAATCAACACCACACAACTCCCTTGAAACATACTAAT11.5384615384615385No Hit
GCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCA11.5384615384615385No Hit
TGCTATGACAATAAGGAGATTGCTCTGCATTGTGGAAGTATGCTTAGGGA11.5384615384615385No Hit
GATCTACGAATAGAGACAGATACTCAACTAATAACCACTACTCCAAGATA11.5384615384615385No Hit
CTTCTTGTCAGTGCTACAAGCCTGCTCTTTAGTCTACGCACACGCTCCAA11.5384615384615385No Hit
ACGCCGGGTGGATTGAGCAAGCTCCTCAAGAGGAGTTAAATCAAATTTCG11.5384615384615385No Hit
GTCAGCATCCACACGTCCGATCATACGAGTACAGTCTTCAACAGAGCAAG11.5384615384615385No Hit
CTAAGGCTTAGACGGTGGTCCCCCTTTCTCGCGTAAAAGCGATCAGAATT11.5384615384615385No Hit
TAATTAAATTCATGCAGAAAAAAAAAAATAAGAAGAAACTATTAATGATT11.5384615384615385No Hit
ATAAAAATATGGTATTTACCATAAACAACAACAACCAACTCAAAAATAAA11.5384615384615385No Hit
GAGAACAAACTAAATCCCTTAACGAGGCTCCATTGGCGGGCAAGTCTGGT11.5384615384615385No Hit
ATTAAGACCAGGAGCGTATCGCCGACCGAAGGGACAAGCCGACCAATGCA11.5384615384615385No Hit
ATACCAAACACGATGATTCTGTGGCTAATGCAGAAGCCAGTCTTGTTGTC11.5384615384615385No Hit
CCATTGTATGCCAAGAAGTCAGAGATAGTCTTTTGATTCACAAACACATT11.5384615384615385No Hit
GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTCAAATTG11.5384615384615385No Hit
AAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCC11.5384615384615385No Hit
TCTCCTTCCTCCTCGACACCGGCTGGCCAACTGGCATCGACCCACATTTC11.5384615384615385No Hit
CGTCTCCCACTGCAGATCGGAAGAGCACACGTCAGCAAAGGGATAAACCT11.5384615384615385Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCAAGGGAGTGAAGCCAAGAAGGATGCGAAGCAAAGTCTTCAGAATCA11.5384615384615385No Hit
GAATAGGGGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACG11.5384615384615385No Hit
CGAAAGCAGCTGGTTTTATACCAAGTGTATTGAATGTTGATCTTATTGAC11.5384615384615385No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT11.5384615384615385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACT50.0145.05
GAGAACA50.0145.01
AAACTAA50.0145.07
ACTAAAT50.0145.09
AACAAAC50.0145.04
CCGCCAT50.0145.0145
AGAACAA50.0145.02
AACTAAA50.0145.08
GAACAAA50.0145.03
CAAACTA50.0145.06
Produced by FastQC (version 0.11.5)