FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTAATTATCTGGGTGATTCAGAATTTGTACTCACAGTAGGTTTCTT13.0303030303030303No Hit
AGCTAACACTTCCCGAGGCCCCCGCCGACGTCTCCGGCCACCCTAACGAT13.0303030303030303No Hit
CTGCGGATGTTGGTCTTGCCGCTGACAAATGCAATGTTTTGTTTAACATT13.0303030303030303No Hit
GATCGGAAGAGCACACGTCTCCCTGTCAAGATCGGAAGAGCACACGTCTC13.0303030303030303Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCCTGATAAGATCGGAAGAGCACACGTCTCCCTGATAAGATCGGAAGA13.0303030303030303Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGTGCTGATGCGCACCGGTGAAGCGTTTCAGGCGCTGCGACGCGTGAAC13.0303030303030303No Hit
CTTCCGATCTTCAGAGGGAGACGTGTGCTCTTACGCTCTTCAGAGGGAGA13.0303030303030303No Hit
CAGTAGCGGCGGCGGAGGAAATGGTGACGGGAATGGTCGTAATCGGAATG13.0303030303030303No Hit
GTGCATTGGAGGTCTTCTTTGCGTTGTGGTTCAAGAGCTATTAGTTACAA13.0303030303030303No Hit
GACTTCAATCCTGCAGTGCATGCATGAGCCTCAGGCAAAGTTGCGAAATC13.0303030303030303No Hit
CGTCTCCCTGCGAGATCGGAAGAGCACACGTCTCCCTGCGAGATCGGAAG13.0303030303030303Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTCGGCTGGGAGACGTGTGCTCATACGCTCTCGGCTT13.0303030303030303No Hit
CTTCCGATCTAACCAGGGAGACGTGTGCTCTTCCGATCTAACCAGGGGGA13.0303030303030303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGTAGATCGGAAGAGCACACGTCTCCCTGTGTAGATCGGAAGAG13.0303030303030303Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCCGACACAAAGCTCTGTTGGTTTTTTTCCGAGATTAAGAACTGCTGGA13.0303030303030303No Hit
CCTCACGTATCACACCAGAACCAGATACCGACCCGAGCGCTGTAAAGCCA13.0303030303030303No Hit
GGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCA13.0303030303030303No Hit
TAGTTATAGCAATATCAGTCCCAACATTTCGAAAATCCAAGTTGTACTCC13.0303030303030303No Hit
GTCGTGAGATGGGACAACACTTCGAGTGTTTCTGGGACATCATGAAAGAC13.0303030303030303No Hit
GTCTTGACCGTCATCTTTTGCCCGAAGGACGGATGAGCTTTGCCGGCACT13.0303030303030303No Hit
CGTAGTACAACTAAAGGGAGAAGAAACAATAAAACATTACAACACTAAAA13.0303030303030303No Hit
GGTTTCGAGTACGTAAACCCAACAACCTGGACCAAGAACACAACGGGAAC13.0303030303030303No Hit
CAACGAGGGACTCGGTGAAATTGAATTGGCTGTAAAGATGCGGCCTACCC13.0303030303030303No Hit
TCTTTGTTTCCTGTGATTGTGCATATGCCGTGGCGGCCAGCTCGGACATC13.0303030303030303No Hit
CAGAAAGACACCACATATGGCTCAACTCCCCCTTTCTTAATACCATAAAA13.0303030303030303No Hit
CGTCTCCCGTCTGAGATCGGAAGAGCACACGTCTCCCGTCTGAGATCGGA13.0303030303030303Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTCCCAAACTAGATCGGAATAGCACAAGTCTC13.0303030303030303No Hit
GACGTGTGCTCTTCCGATCTGACCTGGGAGACGTGTGCTCTTCCGATCAG13.0303030303030303TruSeq Adapter, Index 3 (95% over 21bp)
ACGGTGTACAACGTGTATGTGAGCAGCCAGCGTTCGCCGATCGCGAGTTT13.0303030303030303No Hit
CTCTTCCGATCTGCTGTGGGAGACGTGTGCTCTTCCCATCAGCTGTGGGA13.0303030303030303No Hit
GACGACGAGTGGCCGCGCCTGACCGAGGCGATTGAAAAGCTGCGCACAGT13.0303030303030303No Hit
AATACGCACCGTGAAACCGTGGCCTATCGATCCTTTAGACCTTCGGAATT13.0303030303030303No Hit
CTTTGAAGACAACGGCATCTCCGTCGGGCTGGTGTTCCTCCTGCGCCAGC13.0303030303030303No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)