FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATGGGGCGACCCCAGGTCAGGCGGGAGAACCCGCTGAGTTTAAGC13.571428571428571No Hit
GATCGGAAGAGCACACGTCTCCCCGAACAGATCGGAAGAGCACACGTATC13.571428571428571Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATTCCATTGTACTCAGGTTGATTCAATTCCATTGCATTCCATTCCATTCC13.571428571428571No Hit
GGCCGGGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAG13.571428571428571No Hit
GAGCTAATACGTGCAACAAACCCCGACTTATGGAAGGGACGCATTTATTA13.571428571428571No Hit
CTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGACGT13.571428571428571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGCACACAACTCAGACTCCTTGGTCACTGTTACAACAAGTGTCTAA13.571428571428571No Hit
CACGTCTCCCTAATGAGATCGGAAGAGCACACGTCTCCCTAATGAGATCG13.571428571428571Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTACATAGGGACATCGAGTGCGTAGTCAACATACAGCTCAAAAACAATAG13.571428571428571No Hit
GAAGAAGGGAAACGCGTCTGGAGTTAGCGATTCAGCCGCAGATAGATACG13.571428571428571No Hit
AGGGAGACGTGTGCTCTTCCGATCTCATAAGGGAGACGTGTTCTATTCCG13.571428571428571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATCCAAGATCTCCTCCTTTTTTTCCTGATGGCCACTCAGAGTACACTG13.571428571428571No Hit
ACAATGCTCCGATTTGTTTCAGAGACATAGTCTTACGCTTGACTGCACCT13.571428571428571No Hit
GAAGGGGCAGCGGTCGGGGTTGCCGTACTTGCGCAACCAGCGGCAGCGCG13.571428571428571No Hit
TCTATCCCCAGGGAAGATCGGAAGAGCACACGTCTATCCCCAGGGAAGAT13.571428571428571Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCTGACACCGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCG13.571428571428571No Hit
CTCGTCACCAGGTCGAACAAGATGTTGTACTGACGACCCATGGTGAGACG13.571428571428571No Hit
TTGGTGTACGTCGCGATCAGGTTGAACCCCTCAGCGACATTGGTCACAGC13.571428571428571No Hit
TCCCCATGCAGATCGGAAGAGCACACGTCTCCCCATGCAGATCGGAAGAG13.571428571428571Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACATTTACTGCTGTATTTTCCAATGGGATGCAACCAGCTGAAAGAAAGA13.571428571428571No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCG13.571428571428571No Hit
CCTAAGAAGTTTTCTCATCCTAATGTATCCAGAGCGTAGGCTTGCTATGA13.571428571428571No Hit
GAACAGCGTCAAGCTCTACTGAACAAGTTGTTCCAAGGCCTGCACTCTGA13.571428571428571No Hit
CACCAGGGAGTGCCAGACAGTGGGCACAGGACAATGGGTGCAGTGCACCG13.571428571428571No Hit
GTCGAGGCCATGGCTACCAACAACGACAAACCGTTGATTCTTGCTATCTC13.571428571428571No Hit
GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC13.571428571428571No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCGCGCCCGGTTATGGG13.571428571428571No Hit
TGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13.571428571428571No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)