FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAA21.3793103448275863No Hit
CATCACCCTTGGCCGGCTTTCGCACAGCCGGACTCCCATCAAAAGCTGGT10.6896551724137931No Hit
ATCTAGTAGCGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGGA10.6896551724137931No Hit
GGAAAGAATCAAGCTGTTCAAATGCAGAGCCGGTGATTACGCTTCCGAGG10.6896551724137931No Hit
TTTCTACAACGGGAGCTAAACTATCTAGATCGGAGCATTCAATGGCTGCT10.6896551724137931No Hit
ATGTTATCCCATGCTAATGAATCCAGAGCGTAGGCTTGCTTTGAGCACTC10.6896551724137931No Hit
GTAGGGGCCTTTAGTCTTTTGATTAGAGTAGGGGTCGCGAGAGAGCAGAG10.6896551724137931No Hit
ATCTAAATCCCTTAACGAGGATCCATGGGAGGGCAAGTCTGGTGCCAGCA10.6896551724137931No Hit
GTATCAAAATTCTCATCGGAAAAAGACAGATCTACAAATTTTGAGAGACA10.6896551724137931No Hit
TATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGT10.6896551724137931No Hit
CTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCC10.6896551724137931No Hit
ACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAAT10.6896551724137931No Hit
TCTTTTTGTTGCTGGTCTTAGGTTTAGCTGGTTGATAATCTTCGAGTCTC10.6896551724137931No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAG10.6896551724137931No Hit
CAACTTTCGTGATCGAGTCTCGGCATGGGTTCAACAAACAAACAATATGG10.6896551724137931No Hit
TCAGAAGAACCATCCAAAAATACATACCTAATTTTTGTTATAAGAAAGAC10.6896551724137931No Hit
GACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGCTGTCTCTTATA10.6896551724137931No Hit
TCGGAGTAATGATTAACAGGCACAGTCGCGGGCATTCGTATTTCATAGTC10.6896551724137931No Hit
AATTAAGACCAGGAGCGTATCGCCGACCGAAAGGTTCAACTACGAGCTTT10.6896551724137931No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC10.6896551724137931No Hit
GCTGATAGGCTTCAGACTGGTATGAGAGGTGCTTTTGGTAAAGCTTTGGG10.6896551724137931No Hit
GATGGGAAGCCTAAGATCAATTATGTCACGGTGTTTGAGCGTCCTGGATT10.6896551724137931No Hit
TCCTTACGGTTCCTCTAACGGTGTAGCCTCTCTCTAAGAGTAACTTAACG10.6896551724137931No Hit
TTGCATAACAGATGTGTCGAGGTATATTCCTCCACCAGTGGGTTGGTACT10.6896551724137931No Hit
ATCACCTACCACTGTCTGAATCCTACCAGCAATACTCCTCAACCTGTCAT10.6896551724137931No Hit
TGATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTA10.6896551724137931No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10.6896551724137931No Hit
TCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTG10.6896551724137931No Hit
CTCCTACTGAGGGTCGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAG10.6896551724137931No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT10.6896551724137931No Hit
CTGCTTACCTCATACAAACAAGTCAAAATCGGCAATCCTCTTGAGAAAGG10.6896551724137931No Hit
GACGTGTGCTCTTCCGATCTCGCCTGGGAGACGTGTGCTCTTCCGATCTC10.6896551724137931Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AACAAGATGCTTTCGTGTGCTGGAGCTGATAGGCTTCAGACTGGAATGAG10.6896551724137931No Hit
CTACTCATATCCCAAACGGGTTATTACCCGATTCGATTGTGAACTCTGGT10.6896551724137931No Hit
CTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGC10.6896551724137931No Hit
GTTCAATGCGGTTCTGGAGAATGCATGTAGTGAGATGGGAGCAAGAATGT10.6896551724137931No Hit
GTTAAAAGTAGTGGTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACA10.6896551724137931No Hit
CTTCACTTGCTCAATCAGAGAAGGCTGTTCAGGAGCTTCTTCTTCAACAG10.6896551724137931No Hit
CCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGC10.6896551724137931No Hit
GTATAAACACAGAGTCATGTCTGGTTCTGGTGCTGTATACGCGTTATCAG10.6896551724137931No Hit
CTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACG10.6896551724137931No Hit
GGTTAAGTCTGTTGAGATGCACCACGAGTCTCTTCTTGAGGCACTTCCCG10.6896551724137931No Hit
ACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACG10.6896551724137931No Hit
CCAGTAGATTTTGGTATACGCACGAAGAAGAATAATCCACCAGAACCCTG10.6896551724137931No Hit
GGGCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCC10.6896551724137931No Hit
GAGTCAATGGTGAGCTTCATCCTTCTCGCTTCTGCGAAAAGGATTTACTC10.6896551724137931No Hit
GGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTC10.6896551724137931No Hit
CTCAAACAATCGACAAGAACAGACAACTTCAGATGATTCAGAGTTCCAGA10.6896551724137931No Hit
CCCATAGCCTACAAGTTGTGTCTTCAAAGTTTAACAGCACTCATATAAGT10.6896551724137931No Hit
CCTGGGGTGAACTCCTTCGACGCATTTGCGTTTGGCGTTCCTTCGCAGTC10.6896551724137931No Hit
TCTCTCGGGAGCTTTCCCCGGGCGTCGAACAGTCAGCTCAGCTGTCTCTT10.6896551724137931No Hit
TTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTT10.6896551724137931No Hit
GTTAAAAGAAAAGAGAGCCAAAAGTAATCGCAATACATGAAATTGAAATC10.6896551724137931No Hit
GGTTTGTCCCGGTGCACAACTTGGTATCAATTTGGTAACTTCGATGATGA10.6896551724137931No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG10.6896551724137931No Hit
CACTCTTGGTCTAGCAATCCAAGGACCTGAGTAACAATTACGACTGAAAT10.6896551724137931No Hit
ATCTTTCAAAGCATCAAGGTTTCTCGAACACTCTCGCAGGTAACCCAACA10.6896551724137931No Hit
ATTGGAGCTGGAATTTACCGCGGCTGCTGGGACCAGACTTACCCTCCAAT10.6896551724137931No Hit
CTACTGATGAGACTCCTGAGCTCATGCCGTTGACTCATGTCCTAGCCACC10.6896551724137931No Hit
CAAAGAAACAGCGGATTTTGACAGTGTTGTTTAGCACTTTTGCATCACCC10.6896551724137931No Hit
CTCATGAAGACTGAAGCTATGGAACGGGAAGTCCTGGCCGTTGATTCAGG10.6896551724137931No Hit
CAGGGGTTGTACCCCGCCGCCGATTCACGGAGTAAGTAAAATAACGTTAA10.6896551724137931No Hit
TTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCC10.6896551724137931No Hit
CTGTTACAGAGAGCGAAGATGGCTTGGTGAGTTTGGCGTTGATTTGCTCC10.6896551724137931No Hit
CCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGG10.6896551724137931No Hit
CTTCCGATCTCTAAACGGGGGATCACCTTCACCTTCAACATCAAACATCA10.6896551724137931No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT10.6896551724137931No Hit
GGCTTTTTACTCTCTTCATCCTCATCTTCTTTTGCAGGCTCGCCAGGTTG10.6896551724137931No Hit
ATGGTACCCCATTAACACCATTGCTCGAACTACGCAAGTAATCTGAAGCA10.6896551724137931No Hit
CTTCCATTCTCTCACACTATCTTCACATGTCTCCTCTTTTATCTTCTCGT10.6896551724137931No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT10.6896551724137931No Hit
TTGCTTACGCAGCTAACGCCGATAAGATCTCGTTGGATTACGCGTTGTTT10.6896551724137931No Hit
TCAATATTCCATCCTTTGCAGTAGTAAAAAGGGACTTCACTAATCACTTG10.6896551724137931No Hit
CCTAATGCCTGTAATCCACTGATAAATTCTTGCCTGGAAAACTCACACGC10.6896551724137931No Hit
AGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTA10.6896551724137931No Hit
GGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTC10.6896551724137931No Hit
ATTCTGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT10.6896551724137931No Hit
ACATAGAAAGCCATGATTTTGTTGGAAGAAGATGGGTTTGATTATTTGCT10.6896551724137931No Hit
GGTGCACAAGTACTCCTGTGCTCTCCTTCCTTCTCCTGATAACCCCAATC10.6896551724137931No Hit
GTTCACCACCGCATGTCGGTACGCTCCAGGCGTCCTTGGCTCGGATTTAG10.6896551724137931No Hit
GAATGTAGCGGTCTTTTGGAATATAAATCCCATTCTTCTCTCTTGCAGCA10.6896551724137931No Hit
GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA10.6896551724137931No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC10.6896551724137931No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG10.6896551724137931No Hit
GAATGGAGGTGGTGATAAAAAGGAAGCAAGTGCATCTGCGTTTTTGAGTC10.6896551724137931No Hit
GGGTCAACAAGATGCTTGCTGTCCTAGAGCAGGAGGATGAGACCACAAGC10.6896551724137931No Hit
GAATAAATCAGTCATATACAACAAATCAAATACTATAATCTCATTGTTCT10.6896551724137931No Hit
GCCGCGCCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGT10.6896551724137931No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT10.6896551724137931No Hit
ATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGG10.6896551724137931No Hit
GCTGTCAGTATGTCCCAAATGAGGATGCCCACCTTATCACCAGTTCAGGT10.6896551724137931No Hit
CTATATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACT10.6896551724137931No Hit
GATTAGAGTGCCACAGAGACAAAGCAGTCTCATCATCACCCTTATCAGAT10.6896551724137931No Hit
GTTATCAACCGTCGACGACCTCGTGTCGATTCTAACCACTCTAATCTGGC10.6896551724137931No Hit
GGCTTCGGCGGGCGAAATTGTAGTCTGGAGCCGCGTCCGCCGCGACTGAC10.6896551724137931No Hit
GTATGAGGAGTGCCGCAAGAAGTATAGAGCTATTGGAACGTTCATGAGTG10.6896551724137931No Hit
GTATATATTCCAATCAGTTGGCTTCGCCTTTCACATTATTTCCCCATCTA10.6896551724137931No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC10.6896551724137931No Hit
CGTTATGGTCAACCGATACAGACCGACGAAAGCAGATAGAGCAGCAAACC10.6896551724137931No Hit
TAGCAAACGTCTCCCGAGTGACATCGGAAGAGCACACGTCTCCCCAAGTA10.6896551724137931Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTCTAGCACCACTTGCATTTCTGCTAATATGACCATGGGCCACATTTCTC10.6896551724137931No Hit
ACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTT10.6896551724137931No Hit
GGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTG10.6896551724137931No Hit
GGACCGGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAG10.6896551724137931No Hit
TTCTTACAGTACTTGGCTTCCTCCTATTGAAATATCTGATCAGGGTATTG10.6896551724137931No Hit
TATAATACTAATGAGCTTTTATTTGTCTAATTATGCAATCTTGCCTTGCT10.6896551724137931No Hit
GCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACC10.6896551724137931No Hit
TGCTCAGACTTGGACTAGTGCGGCTTTGACTGACGAGAACACTTGCTCCG10.6896551724137931No Hit
GGTTACGCCGGTGAAGATGCTCCCAAGGCCGTTTTCCCTTCCGTTATTGG10.6896551724137931No Hit
CTTCCGATCTACCCAGGGACAAAACCAGAAAAAACTACATTTCTAACATA10.6896551724137931No Hit
TCTCTCTCTGTGTCTGTGTATGTGTGGCTTTACCTTTTGTACCTAAACCT10.6896551724137931No Hit
GTGTTCCAGCTTTCTTGAGAAAGTATTCGGAGAGAGTACCGGAGAGAATG10.6896551724137931No Hit
TTTAAAGACACCATGAAGTATCTTTGGATGCCTCGGCTCGTAGAAAGGAT10.6896551724137931No Hit
ATATATATCTACTGCATTGGTGATAGCGTTTTCGGTGTAAGTTGTGCGTG10.6896551724137931No Hit
GCGTTAAGCCCTCGTTTTTCCCTCAGTAAATTTGGGCAAATTTGCTTCTT10.6896551724137931No Hit
GCCTTTGTGGGTGTCTTCACTTTCGTTTTGTACATAGAAGTCATAAAGAA10.6896551724137931No Hit
GATTCTGACTTAGAGGCGTTCAGTCATAATCCAGCGCACGGTAGCTTCGC10.6896551724137931No Hit
GGAGAAGTCCAGCTGCTGCAAGACACCAGAGCAGAAAGTTGCTGTTTTCC10.6896551724137931No Hit
CTCCTGCATTGCACGAGGCAGATATTGGTCTTGCTATGGGAATAGCAGGA10.6896551724137931No Hit
GCCATGGCCATCTCCAAGCCAAAAAAAAAAAGAAAAGAGAAACACTTTTC10.6896551724137931No Hit
GACCGAAGGGACAAGCCGACCAATGCACACCAAAGGCGGACCGGCCGACC10.6896551724137931No Hit
ATTCCTCTTTCTCTCTATTTATAGTGTTTGATCCTACTCTCTTATGCTTG10.6896551724137931No Hit
GAACAAATAGGGTGGGTAGGCCCAACCACCACACTACACGTATAGACGCG10.6896551724137931No Hit
ATTCTAAGGGGAAGGTGTTACCTGAAATGATTTTGATATGCCAGAATCTT10.6896551724137931No Hit
CATTAGTACCAGGCTCTTGATTCCCAAACTGGTTACACGGAAACGCAAGA10.6896551724137931No Hit
CTTCCGATCTCATCCGGGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGG10.6896551724137931No Hit
ACAAAAGGGTAAAAGCTCGTTTGATTCTGATTTTCAGTACGAATACGAAC10.6896551724137931No Hit
CCCTACACATCTTTTTCGTATCCTGCGCCTCTCCCGCTAACTTCCACCGT10.6896551724137931No Hit
ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG10.6896551724137931No Hit
GCGCTGGGTGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCA10.6896551724137931No Hit
GCTGAAACCCGCTATTAGAAACGCGGCCAACATGCCAGATCTGACAGAAG10.6896551724137931No Hit
GTGCGGGAGCTGGAGCGGGTGCGGGTTGCTTCGTCTTTTCGGGTTCTTTG10.6896551724137931No Hit
ATCTTCTCCGGCAACGACACTTCTTCCTCCTTGACCGAAATATCACTCTC10.6896551724137931No Hit
GGTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAG10.6896551724137931No Hit
TCCTACACCTCTCAAGTCATTTCACAAAGTCGGAATAGAGTCAAGCTCAA10.6896551724137931No Hit
CCACAAGCGTATCAAAAGCCCGGGATTAGGCCGCCACCGTAATCCGCGTC10.6896551724137931No Hit
CACATGTCAGACTCCTTGGTCCGTGTTCCAAGACGGGTCGAATGGGGAGC10.6896551724137931No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGG10.6896551724137931No Hit
ACCTATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAG10.6896551724137931No Hit
CAGTTGGAGGATGGCAGAACTCTTGCTGACTACAACATCCAGAAGGAGTC10.6896551724137931No Hit
ATCCACGAGATCAAGGACTTCCTTCTGACAGCTAGAAGGAAAGATGCACG10.6896551724137931No Hit
GCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGA10.6896551724137931No Hit
GCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGA10.6896551724137931No Hit
GCCGCACTAGTCCAAGTCTGAGCATTACTCATGTGGTACGCAAACTGGTC10.6896551724137931No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)