FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCC21.9607843137254901No Hit
ATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACAT21.9607843137254901No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA21.9607843137254901No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA10.9803921568627451No Hit
GCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAAC10.9803921568627451No Hit
GATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAG10.9803921568627451No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC10.9803921568627451No Hit
TGCTAACGCAGGCTGGTCTTTCGGCCAGACCTTCACAGGTGCGCAGCTAC10.9803921568627451No Hit
CCCGAATAGATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACT10.9803921568627451No Hit
CCTAAAGAGATCGGAAGAGCACACGTCTCCCTAAAGAGATCGGAAGAGCA10.9803921568627451Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAAC10.9803921568627451No Hit
TCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTC10.9803921568627451No Hit
ATCTACACCGGGGGCTGTCTCTCGGCGTTCTTCAATCGAAAGGTAAAGAG10.9803921568627451No Hit
CCTCGCAGCTTCTGAAGCATTGGCCGCACAAGTGACAGAAGAGCATTACG10.9803921568627451No Hit
ATAAAATACAGACCGAATAAAACCGATGAAAGATATGGCCTGTCTCTTAT10.9803921568627451No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT10.9803921568627451No Hit
GGCCGGTCCGCCTTTGGTGTGCATTGGTCGGCTTGTCCTTTCGGTCGGCG10.9803921568627451No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT10.9803921568627451No Hit
GACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCG10.9803921568627451No Hit
GGTCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGAA10.9803921568627451No Hit
CCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATC10.9803921568627451No Hit
CTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGCT10.9803921568627451No Hit
GCATCCGACCAGGACGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAA10.9803921568627451No Hit
TCCTAGTTGCTGGAACACTAGACGAATCAATCTCACCAATGAAATATTTG10.9803921568627451No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGGGGCAGCCAAGCGTTCA10.9803921568627451No Hit
GTCATAGACAATTCATGTTTTACCCTCTGGAGTTCTTCTGTGGCTGTCTC10.9803921568627451No Hit
ACTTCAAAGAGCTTCTTCAAGAGAGCAGCGTCATTGATGTCTCCTTCAAC10.9803921568627451No Hit
AGATCGGAAGAGCACACGTCTCCCGGGAGAGATCGGAAGCTGTCTCTTAT10.9803921568627451Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT10.9803921568627451No Hit
CTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGA10.9803921568627451No Hit
ATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCG10.9803921568627451No Hit
GACAACAGAATTGTAGATAATATTTGATCAGGCAGTGATCTCCTTCTTGA10.9803921568627451No Hit
CTTCCGATCTAGCCCGGGGGACTTTGTCAATTCTCTGACAATTGTAATTT10.9803921568627451No Hit
TTGCAGGGGGGCAGTCTCCGACTCATCCAGATACCAAAGTTTAGAGAGAA10.9803921568627451No Hit
GTATTGGAAGTTCCGATGCCGGAGGAGTTGTTCTGTTCGGACAACAACAA10.9803921568627451No Hit
CGCATGGCCTCTGTGCTGGCGACGCATCATTCAAATTTCTGCCCTATCAA10.9803921568627451No Hit
TTGTAGACGTTCTTACACTGGCCACAAGAACAATTCAAGAATTTGGTCAA10.9803921568627451No Hit
GAAGAAGGCTCACATGATGGAGATCCAGATCAATGGTGGTACCATTGCCC10.9803921568627451No Hit
CCGTACTCGAAGACGAAGAAGAAGTAGAAGAAGCCATTGTCGATGAATGA10.9803921568627451No Hit
AAAATGGATGGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGG10.9803921568627451No Hit
ATTTGGGGGTGGTGGCATCCATCTTGTTACAACAGCAAATCATCTGCTTG10.9803921568627451No Hit
GGACAAATGGATTCTTCTTTAAGATACTGTCAACACGGAGCACTCTCTCT10.9803921568627451No Hit
TTTTTGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAAACTTTG10.9803921568627451No Hit
ATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGG10.9803921568627451No Hit
GCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCGTTAGG10.9803921568627451No Hit
CATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCT10.9803921568627451No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG10.9803921568627451No Hit
AGCGTGCTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCTTGAA10.9803921568627451No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT10.9803921568627451No Hit
GTTACCACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAGGGCAG10.9803921568627451No Hit
GTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGC10.9803921568627451No Hit
CTTCGGCGCAAGTGAAATACCACTACTTTTAACGTTATTTTACTTACTCC10.9803921568627451No Hit
CACCAAAGGCGGACCGGCCGACCCATCCCAAGGTTCAACTACGAGCTTTT10.9803921568627451No Hit
TCCCACGGCAGACATTCTAGAAGGATGATTTACTCAAAGATATCATCATC10.9803921568627451No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGTTC10.9803921568627451No Hit
GGATTAGAGCTATTGTTCCCATTGCTAACTACAGTGAAAAGACCAAAACT10.9803921568627451No Hit
ATCTTAGTCTCACCGAATATCACATAGGTCTCAGAGTTCGGACTCTTGAA10.9803921568627451No Hit
CCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAAC10.9803921568627451No Hit
TTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGA10.9803921568627451No Hit
TCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAAC10.9803921568627451No Hit
GAACTATATCGTCCCTATAAACATCTCATACATCCATCAATCAACTTAAC10.9803921568627451No Hit
TTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCA10.9803921568627451No Hit
GTGCTCTTCCGATCTAACATGGGAGACGTGTGCTCTTACGATCTAAAATG10.9803921568627451No Hit
GCTATCCACTCTCATGTCGTTAGAGTCCGTTCCCGCACCAACCGCAGGGT10.9803921568627451No Hit
ACATGGAAGATCTGTTCCAGGAGAAGAAGCGTGTCAGGAATCCTCTCGTT10.9803921568627451No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAA10.9803921568627451No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT10.9803921568627451No Hit
GATCTGACAAGGGGGCCGGGTCGTACCTCCGGCGCTGGTACTTTGAAGAA10.9803921568627451No Hit
AGTGTACCAGTTGTCAATGGTCTGACTGATCATAACCATCCTTGCCAAAT10.9803921568627451No Hit
GATAAGGAGAATTTGCGGTCTGGACAGTTAGCCTTCCACGAACCATATCC10.9803921568627451No Hit
GAGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGC10.9803921568627451No Hit
CCCACGTATGTGGCAAAATACGGGGATGACTTGTGGCTAGGGGTGAAAGG10.9803921568627451No Hit
AATGGTGGGTGAACAATTCAACACTTGGTGAATTCTGCTTCACAATGATA10.9803921568627451No Hit
CACCGGACACCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACC10.9803921568627451No Hit
CCCTTAACCATGGCTGTCGGGAAGAACAAGAGGATTTCAAAGGGTAGGAA10.9803921568627451No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTT10.9803921568627451No Hit
GATTCACGGAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGC10.9803921568627451No Hit
CCGTCACCCGTTACCACCATGGTAGGCCACTATCCTACCATCGAAAGTTG10.9803921568627451No Hit
GACCGAAGGGACAAGCCGACCAATGCACACCAAAGGCGGACCGGCCGACC10.9803921568627451No Hit
ACCGTGCGCTGGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAG10.9803921568627451No Hit
TCGTGGTGCATTGGAAGTCCGCTGGCTGGCCGAGCGTGACGCGGCGCTGC10.9803921568627451No Hit
GATCTGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT10.9803921568627451No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10.9803921568627451No Hit
CGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGAACA10.9803921568627451No Hit
CCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGC10.9803921568627451No Hit
CTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGCGCAC10.9803921568627451No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC10.9803921568627451No Hit
GCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAA10.9803921568627451No Hit
CCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAA10.9803921568627451No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGACATCTATTTAGTGAC10.9803921568627451No Hit
GTGCACCCGCGAGGGATCCCGCCAATCAGCTTCCTTGCGCCTTACGGGTT10.9803921568627451No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT10.9803921568627451No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTT10.9803921568627451No Hit
TGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGA10.9803921568627451No Hit
ACAATATCCTTTCCCTCATCGAATCCAAGCCTCATCTGGGCGGTGAGACC10.9803921568627451No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAA10.9803921568627451No Hit
GTCTTGAATGTGGATTGTCTCAAAGCTTCCCTTATGCTTTTCACGGTTCT10.9803921568627451No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAG10.9803921568627451No Hit
GTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGC10.9803921568627451No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG356.259579E-420.714287140-144