FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT11.9607843137254901No Hit
GATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAG11.9607843137254901No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG11.9607843137254901No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG11.9607843137254901No Hit
GTTCAACTACGAGCTTTTTAACTGCAACAACTTAAATATACGCTATTGGA11.9607843137254901No Hit
CGTCTCCCAGCGGAGATCGGAAGAGCACACGTCTCCCAGCGGAGATCGGA11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTCTTAGCCGATTCCTTGCCCGCCGGATCCGTGGTTTCGCGTATCGGCAT11.9607843137254901No Hit
ACCAAGAGGAGAGGAAGAGACGGACGATCGGACAAAGGAAAAGTGCTGGA11.9607843137254901No Hit
ATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGATGCGTCCTGGTCGGATG11.9607843137254901No Hit
CCCCTGTTTTTGGTCCCAAGGCTCGCTTCGGCGGGTCGATCCGGGCGGAG11.9607843137254901No Hit
CCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGC11.9607843137254901No Hit
CCACGATCACGGCAGCGACGTCTCCACAAGCGTATCAAAAGCCCGGGCTT11.9607843137254901No Hit
CCATGGTGGTAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGA11.9607843137254901No Hit
GAGATACAGAAGGAAGCGGACATCATCAGCAGCTGCGCGGATCATGAGCT11.9607843137254901No Hit
GTAATACACTACTGATACATCCAACGACCACCGTCCGATTAGGGATGATC11.9607843137254901No Hit
GGAAAAGGGGCTGGATTCGGGTTTGGTTCGGGGTCAGGTTCAGGAACTGG11.9607843137254901No Hit
GGACAAGCCGACCAATGCACACCAAAGGCGGACCGGCCGACCCATCCCAA11.9607843137254901No Hit
GAGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGC11.9607843137254901No Hit
ATCAAATTCATCTTTACCATGCTTCTTCTTCGCTTTCCTAACGACTGGCT11.9607843137254901No Hit
CTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATC11.9607843137254901No Hit
GACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGAT11.9607843137254901No Hit
CCACTATCCTACCATCGAAAGTTGATAGGGCAGAAATTTGAATGATGCGT11.9607843137254901No Hit
AAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATT11.9607843137254901No Hit
ACTAAAGGCCCCTACTGAAGATCGCCAAACTACGACGAGACTTTCGCCTT11.9607843137254901No Hit
CATAAGTCGGGGTTTGTTGCACGTATTAGCTCTAGAATTACTACGGTTAT11.9607843137254901No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11.9607843137254901No Hit
CAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACG11.9607843137254901No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC11.9607843137254901No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTAGTGAAATGAC11.9607843137254901No Hit
AGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGCCAATCAGCT11.9607843137254901No Hit
TGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCC11.9607843137254901No Hit
TGATAGGGCAGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGA11.9607843137254901No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAA11.9607843137254901No Hit
CAATAGACCAGGTTGTTTTGTTGGAACCAAACAACATCGAAGCATCACCT11.9607843137254901No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA11.9607843137254901No Hit
ATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTC11.9607843137254901No Hit
CATAAGCATGGGATAACATCATAGGATTTCGATACTTTTGTGTTGGACTT11.9607843137254901No Hit
AACCAAGCGCGATGACCAATTGTGCGAATCAACGGTTCCTCTCGTACTAG11.9607843137254901No Hit
GGGCACGTCTGCCTGGGTGTCACAAATCGTCGTCCCTCACCATCCTTTGC11.9607843137254901No Hit
GGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAA11.9607843137254901No Hit
GCTCTCACCCTCTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGT11.9607843137254901No Hit
GGATGTTCTAAAGAGCACACGACGAAACTCCTCTTTGTATATGAAGTGAT11.9607843137254901No Hit
ACACCTAATGGCTTAAGCCGCTCTTCCCATTTCCTCGCTGACCCATCATG11.9607843137254901No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT11.9607843137254901No Hit
GCGAAACCCTCGTCTTGTGTTGCTCAGACATGCGCCTAAGGAGAAAGGCT11.9607843137254901No Hit
ATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATCTAATAAATG11.9607843137254901No Hit
GCCCACAGGCCCACGCCCGGAGCACGCTGATGCCGAGGCACGCCGTTAGG11.9607843137254901No Hit
TCCCTTCTCTGCGTTCAGCGTTCGTCGTCACCAAGGTTAAAAACCAATTT11.9607843137254901No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT11.9607843137254901No Hit
AGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAGAGGC11.9607843137254901No Hit
TGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAA11.9607843137254901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCA50.0145.08
TAATCGA50.0145.05
GTCTTAA50.0145.01
CAAAGCA50.0145.0145
CTTAATC50.0145.03
TTAATCG50.0145.04
AATCGAC50.0145.06
ATCGACC50.0145.07
CGACCAA50.0145.09
TCTTAAT50.0145.02
Produced by FastQC (version 0.11.5)