FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGATCTTACCAGGGGGAAGTCGAAATCCGCTAAGGAGTGTGTAACA13.7037037037037033No Hit
CTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAG13.7037037037037033No Hit
AGTCTCAACGTTGTTGCAAAGACACCCCGACAGTCTCAACTCGATGTGGT13.7037037037037033No Hit
TCCCTTCACGGCCGGCTTCTTAGAGCGACTATAGCCGTTTACGCCAAGGA13.7037037037037033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13.7037037037037033No Hit
CGGGATGCTTATACAGAATGCAAGTGCAACAGCTCTGTCCGCCTGGTCCA13.7037037037037033No Hit
GTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGTCAT13.7037037037037033No Hit
TCCCAGCACAGATCGGAAGAGCACACGTCTCCCAGCACAGATCGGAAGAG13.7037037037037033Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGGAAGGATCACCACTTAGGTTTACCAAGCACTCAAAGTCAATCTCGTC13.7037037037037033No Hit
CGTAAGGGACCGATTATAGATCATCAAAATCCCACAAAACGAATGAAAAG13.7037037037037033No Hit
CTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACC13.7037037037037033No Hit
AATATGACAAGAACGGTAACCTCAACGAACACCTTTCAATAGGGGTAGTC13.7037037037037033No Hit
AGCCCGGGCGGAGCGCCCGTCCGTGCACATCATGCTGGAAGTACCAAAAA13.7037037037037033No Hit
TTATTACATAATTTTATTAGTAATTAAAAAATAAAGCGAATGACAAATGA13.7037037037037033No Hit
GTTACCCGGGTGCCACGAGCTATTTACGCGTATTTACCAATTCTAAGGTA13.7037037037037033No Hit
GAAGAGCACACGTCTCCCAGCAGAGATCGGAAGAGCACACGTCTCCCAGC13.7037037037037033Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAACAGTAACTGCAAAGGCCCTGAGACAAGAGCATTCCTGGTGTACTAGG13.7037037037037033No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTAGAGCCGCCGA13.7037037037037033No Hit
CGTCTCCCGGGCTAGATCGGAAGAGCACACGTCTGCAAAAAAAAAAAAAA13.7037037037037033Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CTGTCGAGCTCTGAACAAGCCGAAACATTCAGAAGAGCATTCACTTATTG13.7037037037037033No Hit
GAGTTACTTCAAAGGCACAATACCAATTATTCGATCCAGCAAAAGCGTCT13.7037037037037033No Hit
GTCTGAAATTGTTGACGGCTCTTAATCCCAACATCATGTCGCTCAATCCG13.7037037037037033No Hit
AGTCAGATCGGAAGAGCACACGTCTCCCTTGTCAGATCGGAAGAGCACAC13.7037037037037033Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAGCCCGGATCACCAGCT13.7037037037037033No Hit
ACGTAACAGTAGGTGCAATTGATGTTGCACCTACTAGCCACCTTCACTAA13.7037037037037033No Hit
GCCCTGCGTAGATCGGAAGAGCACACGTCTTCGCTGGGGTGGCAGAGCCC13.7037037037037033Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTCTCGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAGGGG13.7037037037037033No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers