FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGATCTTGTCCGGGGGTTAATATTCAATTGGCGATACTATATTCTT22.1739130434782608No Hit
GGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACA11.0869565217391304No Hit
GAGCCCCACCAAGATCGGAAGAGCACACGTCTCCCTGCTCCTGTCTCTTA11.0869565217391304Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAA11.0869565217391304No Hit
CTTGGGAGGTGAGGACTTTGACCACAGGATCATGGAGTACTTCATCAAGT11.0869565217391304No Hit
CAGGTAGAGCTTTCTCCGCCGGTGGTGATCTAAAGGTGTTTTATCACGGA11.0869565217391304No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT11.0869565217391304No Hit
AGACGTGTGCTCTTCCGATCTACGTTGGGAGACGTGTGCTCTTCCGATCT11.0869565217391304Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTTCTTCTTCATCACCATTTTTTTCTGCGCTCGGATTTCGAATTCGAAC11.0869565217391304No Hit
GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA11.0869565217391304No Hit
TCAGTGAGCAATGGCAGAGTTGGGTCAGCTAATAAACCTTTCTTGAAGTT11.0869565217391304No Hit
TGGCCAAACCGCCCACCTTTTGACAGATACTGTTGATGATGCTCTTCAGC11.0869565217391304No Hit
CTGAGGGACAAAAGCGTCAGGCTGCAACAACTCCATGGAACTACCAAACA11.0869565217391304No Hit
CAATAGATGCTGAAGCCAGAGCAGCTGCCATCTCTGCAGCGAGATCCGAA11.0869565217391304No Hit
CTTTGGAACATTGAAGGGACTTCGTTGACTTGGTATTTTTGAATATTTTG11.0869565217391304No Hit
GCTCAGGACATTTGATAAAAGGGATTCTTTGGTGTGGAGATGTGACTATG11.0869565217391304No Hit
GTTGTCACCATTCTTACGGCTCACATATCGGGCAATGGCATTGCTCTCAA11.0869565217391304No Hit
ACCGTGTGGTCCCCCGATCACAAACCGGCCTGATGGGTTGAGGTGGAAGA11.0869565217391304No Hit
TACAAGGCCAGATTCTCGAGGTCACTGTTGTTGGGTGCCAGAAATTGAAA11.0869565217391304No Hit
GTTCATATCCCTGAAGAACCTATTCTTCAGCTTGCGTCTGGGCTCAGAAT11.0869565217391304No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT11.0869565217391304No Hit
TGACAAGATCCTTTGCACGTGGCAGGTTCGACGCCAATCCGAGGTCATAG11.0869565217391304No Hit
GCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCGATC11.0869565217391304No Hit
GTGGAATACGAGGTGAAACCGTGCAAAACCAATGGCGATGGATGCGTTGA11.0869565217391304No Hit
GTCCATGAGTGCATCAAACATGTTGGTGTAGACGTGGCGGGTGAACGGGT11.0869565217391304No Hit
ATGGATTGATCTGGAGTGGCCAATGAAGAAAGTATGGAGACACAATTACC11.0869565217391304No Hit
ATACTGCATCCCGAAGCTCATCTTCATTCAACATCCTGTGAAGTTCATCT11.0869565217391304No Hit
CCTCCAGAGCTGGGATCCAACTCTTGTTAATCCTTGTACCTGGTTCCATG11.0869565217391304No Hit
GCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATCTGTCTCTTATACA11.0869565217391304No Hit
GTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCCCT11.0869565217391304No Hit
AATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTAACGCAATGTGA11.0869565217391304No Hit
GGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCGTGAAGGGATGCCT11.0869565217391304No Hit
CCGGTTGGGCCACCGGAATCAAGCTTGGTAACGACTCACCAGGCACATCT11.0869565217391304No Hit
TCATTTGGTGTGGATGTGAGCCTTGAAGGGAATCTGATACTGTGCAGGCA11.0869565217391304No Hit
GTTAAAAGTAGTGTTATTTCACTTCCGCCTAAGATCCCACTTATACTACA11.0869565217391304No Hit
CTGTATATCAGAGACCAGAGAATAACAAACTAGAGAGATGTTATGGAGGC11.0869565217391304No Hit
CCTCGCGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAG11.0869565217391304No Hit
GGCGATGACCAATTGTGCGAATCAACGGTTCCTCTCGTACTAGGTTGAAT11.0869565217391304No Hit
GTGTTAGCGGAGGTTTTATCAGCGATCTTCGCGGAGGTTATGAGCGGGAA11.0869565217391304No Hit
CCTTTCAACTTTGTTTTCACCTCATCTACCCTGACGTTGTAAATATGTAT11.0869565217391304No Hit
GGCCAATTAAGACCAGGAGCGTATCGCCGCCCGAAGGGACAAGCCGCCCA11.0869565217391304No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATA11.0869565217391304No Hit
CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGG11.0869565217391304No Hit
ACTTAGAGGCGTTCAGTCATAATCCAGCGCACGGTAGCTTCGCGCCACTG11.0869565217391304No Hit
TATGAAATACTGTATGGTGGAAATCGGTGTAGGCATTAGAGCGGGCACCT11.0869565217391304No Hit
GCTCAAAAAGCTTCCTCTTTATATGTGTGTTCCAGTAGTTCTTGATCTCG11.0869565217391304No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAGCTCGTAGTTGAAC11.0869565217391304No Hit
AAGTAGAACATACATATACATACAGAAACACATTTGCACAAATCCCACCG11.0869565217391304No Hit
TTTCTCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGC11.0869565217391304No Hit
GTCTCCCTCCCTTCACATTCGTAACCTCGTTCCAGCGTTGCAACGAGACA11.0869565217391304No Hit
GTTTCAACCAGCGGTGTGGTGTTGACGACTTGTGAATCCTCTGGTTTCTT11.0869565217391304No Hit
CCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTAC11.0869565217391304No Hit
GATGATACCAATGTTTATCGTGTCACGATTCACAAGACATTTGAAGGAAA11.0869565217391304No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGAT11.0869565217391304No Hit
AACCTGAGAAGAACGGGAAGCTGGACCAAGGAAAAGGAGATGATGCCCCC11.0869565217391304No Hit
GTCTTAATTGGCCGGGTCGTGGCGCCGGCGCGGTTTATTTTTAGAAATTA11.0869565217391304No Hit
GAAACAAACACTACAACGTGTAAAGTTTCTGCTTGGTAGAGACTAGAGAG11.0869565217391304No Hit
GAAGTACATATACACTTGCTGAATAACCCAGATGCTAACCACTGATTCGA11.0869565217391304No Hit
ACAACAGATAGTTCAATTCCCATCTCAAACTAGGGAAAATAGATTTTTTT11.0869565217391304No Hit
TAACAAAACTGGTGCCTCCTCTTCCTCCTCCGGTGCGGCTTCTTCCTCAG11.0869565217391304No Hit
TCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCTCAGAAGAT11.0869565217391304No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG11.0869565217391304No Hit
CTTCTCATCATAACAAATCTGCTACATTGCCAATTTGCTTGTACACCTGC11.0869565217391304No Hit
CTGGACAAGAGCCCTGCGAGCCATGCCATTAGCCACCACACTCTTTGCTG11.0869565217391304No Hit
GCTTTATTTAACACAACAACAAAGAACAATACAACAACACACAGACAGGT11.0869565217391304No Hit
AGGAAAACTAGCTGCAGGTGCGCCAAGACGGTATTCTGGAGCGCTGAATG11.0869565217391304No Hit
CTTCATATCCTTGACTTGCAAACAGTAAGTAAGCACATTTTATTGGTATT11.0869565217391304No Hit
TACACAGGAGATGAGATATGCCGTGAGAAGACAAAGGAGTTTCTCAAGGA11.0869565217391304No Hit
GATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGAGAA11.0869565217391304No Hit
AATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC11.0869565217391304No Hit
CTGAATAGCTTCCAAGTTTGTGCTTCCCTTTAGCCTGAAAACAGCATCCA11.0869565217391304No Hit
GGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCC11.0869565217391304No Hit
AGCCTCATGTGCCAAAGAGTTGCAACCTTCGTCTTTCCAGCTCAAGCACT11.0869565217391304No Hit
ATTATATTCAAAACCTAATTTTTCAAAACAATTCTTGAAATATTGTGAAA11.0869565217391304No Hit
CTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTAT11.0869565217391304No Hit
CTGTATTGGGAGACGTGTGCTCTTCCGATCTGTATTGGGAGACGTGTGCT11.0869565217391304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA11.0869565217391304No Hit
CACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCC11.0869565217391304No Hit
CATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGG11.0869565217391304No Hit
GTCCCGGTTCCGGAATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAA11.0869565217391304No Hit
CCATCTGCAAATCCACATTTCTTACAAATAGTGCAGGTACACTTATTTCT11.0869565217391304No Hit
TCATTGTCCAAGGTTGATTTTGCTTCTGCTTACCATAGGCTAAGAGGAGC11.0869565217391304No Hit
CCTTGTGATACCGAAGCTCTTCCTTCTAACACATCTTGTGCAAATGTGGC11.0869565217391304No Hit
GAAGAAGACCGATGGTTCAACTACTCCGGCGTACGCTCACGGCCAACATC11.0869565217391304No Hit
CTCCTCCTCCGTGTTAACCTCAACGAGATCCTTCAACGAAAGCTCGTAAC11.0869565217391304No Hit
GGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGAT11.0869565217391304No Hit
CCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGGCTGA11.0869565217391304No Hit
ACTCAGAGATCTTCTTCTCTTCCACAAGACAATTGAAACCTCTGAATGGG11.0869565217391304No Hit
GCCCGATTGGGGCTGCATTCCCAAACACCCCGACTCGTAGACAGCGCCTC11.0869565217391304No Hit
AAAAAAAACTAACAAGGATTCCCATAGTAACGGCTAGAGAACCGGGAAGA11.0869565217391304No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG11.0869565217391304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG50.0145.0145
AGGGACA50.0145.08
CAGGGAC50.0145.07
ATTAACA50.0145.02
TAACAGG50.0145.04
GGGACAG50.0145.09
AACAGGG50.0145.05
GATTAAC50.0145.01
ACAGGGA50.0145.06
TTAACAG50.0145.03