FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11.8867924528301887No Hit
TTTTGTATTCGTGATAAATACAAATATTTTCGTGGGTTTGTGAAAAGTGC11.8867924528301887No Hit
ATGCTATGAATTTGGGTGTATTATCTCCTGGCATTAACACCGGTACGCTC11.8867924528301887No Hit
GTTTCAGCCTTGCGACCATACTCCCCCCGCAACCCAAAAACTTTGATTTC11.8867924528301887No Hit
GTTCTGACACCATCGACAACGTCAAGGCCAAGATCCAAGACAAGGAGGGT11.8867924528301887No Hit
CTTCCGATCTGACAGGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGA11.8867924528301887No Hit
GTGCCAAAGATCAAGACCAGTTTGCGTACCACATGAGTAATGCTCAGACT11.8867924528301887No Hit
CAGGCATAGTAAAAGAAACTATTTGGCAAGTCGAATGACCCCAAAAGTAC11.8867924528301887No Hit
GTCTAACAACTCACCTGCCGAATCAACTAGAAAAGAAAATTGATTGCGCT11.8867924528301887No Hit
TCACAACTCTGTACGTTTCTTTCTTAGAATGAGTGTCAATCATTTTACCA11.8867924528301887No Hit
GATCTCACTTGGGCCAAGGTTGTCCACATGAGTGAGCTTGAAAAACGTGA11.8867924528301887No Hit
ATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCCGAAG11.8867924528301887No Hit
TCACTAGCTTTCACAAATCTGCCTCTGACACGCAACCTAGTGTCAGCTCT11.8867924528301887No Hit
GTGCATGCAAGTTCTTGAGGAGGAAGTGCGCATCCGATTGTATATTTGCA11.8867924528301887No Hit
GGTCTGAGGGCTGGTCTCGGGGTTCCCAGTTCCGAACCCTACGGGAGTCA11.8867924528301887No Hit
CAACAACTTATGATGATCTCAACACCAACTAAACAAGGTGTGACCACAAC11.8867924528301887No Hit
ACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTGTAGGATAAGTGGGA11.8867924528301887No Hit
GTGGAGAGGCAATGTTCTGCTTATTGGACTACTTTGGCTCCGAATGTGGA11.8867924528301887No Hit
GATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCA11.8867924528301887No Hit
CTTCAATGGTTTGTGTGACTTCCTTAGCTCTGGTCCTGTTATTGCCATGG11.8867924528301887No Hit
CATCAAGACTGAACTTTAAACTTGGAAACTCTTTCAAAAAGACGAATATG11.8867924528301887No Hit
GTTCTCAGAGCTAACGCAAGTAGAAGAAGAAGAAGAAGGTTTCTTGTGGT11.8867924528301887No Hit
CAAATAGATAGTGAGGGCATATGATTATATCAAAGAGACCTCCTTTTGGT11.8867924528301887No Hit
GACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAA11.8867924528301887No Hit
TGATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTA11.8867924528301887No Hit
GGGGCGCACCGGACACCACGCGACGTGCGGTGCTCTTCCAGCCGCTGGAC11.8867924528301887No Hit
CTCTTGAGATGTCTCATGCACAGACAACAAATCAAGCTGTGATCCATCTC11.8867924528301887No Hit
GTAACCACACCCATTTGAGCTTCAATACCTGCAATCGTGTTCTGTTGACC11.8867924528301887No Hit
GTTATGAGTACGACCGGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCG11.8867924528301887No Hit
CATTGAACATGTTTATTATTACTGTGATCACAGTTCTAGAGTTTAACATA11.8867924528301887No Hit
CTTCCGATCTGCAAAGGGTTCTACCCGCCGCTCGGTGGTAATTATAATTC11.8867924528301887No Hit
CGGGTTTACTCACCCGTTGACTCGCACACATGTCAGACTCCTTGGTCCGT11.8867924528301887No Hit
CTGTCCCTGTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGG11.8867924528301887No Hit
GGAAGCTTCACACTGTCATTGTCATTGCACCATGTAGGAAGACGACTTGC11.8867924528301887No Hit
CTCCGGTTCAGAGATCTGAGCCTTATGATCGAACTCAGACTCGAGCGTCG11.8867924528301887No Hit
GTCTGGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG11.8867924528301887No Hit
TTGAAAAGAGAGTCAATGAGTGCTTGCATTTGTCGGGAGGGACGCGGCTC11.8867924528301887No Hit
CCTAGTGACCAACGTTTACGGCATGGACTACCAGGGTATCTAATCCTGTT11.8867924528301887No Hit
CGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAA11.8867924528301887No Hit
GCTTATCCTTTTGAAGATCCTTCTGATCCTAAAGATTTTCGGAAGACGAT11.8867924528301887No Hit
CTTCATTCCCAAACATGACTGCAGCAGAGGTCACACAATTTGTGAATGGA11.8867924528301887No Hit
GATCGGAAGAGCACACGTCTCCCTGACTAGATCGGAAGAGCACACGTCTC11.8867924528301887Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTACAATCTAAAATCCCTTAACGAGGTTCCATTGGAGGGCAAGTCTGGTG11.8867924528301887No Hit
GTCCCTAGCAGAGGAATACAGTGAGAAAGGTGAAAAGCTTATCTCCCAAG11.8867924528301887No Hit
AATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGC11.8867924528301887No Hit
CCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCGGCGGTA11.8867924528301887No Hit
GTAATACATATGCCAATACTTGTGATTCCTAATATAGAAAGTGAAACCAC11.8867924528301887No Hit
GAGCACACGTCTCCCTGCGTAGATCGGAAGAGCACACGACTCCCAGCGTA11.8867924528301887Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCAAGAAGCTAGGGAACATTGTACGTTTTGTCTTGTACATGTTACACAAA11.8867924528301887No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA11.8867924528301887No Hit
GACAGTAGCTAGAACTTTGGTACCACGAATGCGAGTTGTGAAAGGTTCGG11.8867924528301887No Hit
GGCCTAAGCCCGGGCTTTTGATACGCTTGTGGAGACGTCGCTGCCGTGAT11.8867924528301887No Hit
GACCGGGGGCCGCTATCCCATTAGCTTGCAAAAATGTTCACTAGCTCTGT11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCTG50.0145.0145
CACACCC50.0145.06
ACCACAC50.0145.04
ACACCCA50.0145.07
AACCACA50.0145.03
TAACCAC50.0145.02
CACCCAT50.0145.08
CCACACC50.0145.05
ACCCATT50.0145.09
GTAACCA50.0145.01