FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGATCTCTGCTGGTGTTAAGAAGAGGAATTGAACCTCTGACTGTAA23.9215686274509802No Hit
GATCTAGACTGGGACCCGCCTGAGCCTCCCAAAGTACTACGATTACAGCC11.9607843137254901No Hit
CTTCCGATCTAATAAGGGAGACTTGTTCTCTTCCCATCTAATAAGGGTTA11.9607843137254901No Hit
CCGATCTTCAGTGGGATTATTAGATCATACGGTAGTTCTATGTTTAGTTT11.9607843137254901No Hit
ATTTGGAAGAGCACCCCTGGGCAGATCGGAAGAGCACACGTCCCCTGGGC11.9607843137254901No Hit
GCGGCGCCGTACGCTTCCAGCGCCCACACTTCCATTTCCCCGAAACGCTG11.9607843137254901No Hit
GGCAGAAATAGACTAGACAGGCAGAAGAGAGAAGACAATGTGACCAGGGA11.9607843137254901No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.9607843137254901No Hit
GAAGAGCACCCCGTTCGAGATCGGAAGAGCACACGTCTCCCAGCAGAGAT11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGGTGTG11.9607843137254901No Hit
GTATTTCACTTGCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGTCAT11.9607843137254901No Hit
ATAAAAAACAGTCTCATTTGTTAGAAAAACTAATTAAATAAAAGTAGTTG11.9607843137254901No Hit
CAATAATCCATAAATATTTATACAAAAAAGAGTCATAAAAGATTCTAGAA11.9607843137254901No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGATACCGGGAAG11.9607843137254901No Hit
GTGCAGAGATATGTCTCAATCCCCCTGTGGGCACAGCCTAGAAACTGTCT11.9607843137254901No Hit
GGTTTTGACCAAACCACCTCTCGTTGCCCAATCATCAGCGTTCCAAAGAC11.9607843137254901No Hit
CCCCCTACTTCTAGTGGGAAAGAAGCCTGGGGGGCGCAGGATCTGTCTGA11.9607843137254901No Hit
GCCAAGGTGTTAAGAAGAGGAATTGAACCTCTGACATAAAGTCTGTCTCT11.9607843137254901No Hit
GAGCACACGTCTCCCTTACAAGATCGGAAGAGCACACGTCTCCCTTACAA11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAAC11.9607843137254901No Hit
CCCCAATGGTGCTCTGTCTCGACTCGACCAGCTCTCCGGTGCCCAGTTAA11.9607843137254901No Hit
CTTCCGATCTGCGCGGGGGGGGTCAGAGAAAAGGCCAAAGGCTATCCTCT11.9607843137254901No Hit
TCACCTAGGAGACCTGATGCGGTTATGAGTAAGACCGGGCGTGAGCGGCA11.9607843137254901No Hit
CACCTGGGAGACGTGTGCTCTTCCGATCTCACCTGGGAGACGTGTGCTCT11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTATTGCCCCGGCCGTTCACGCCCAGGCCACCGAGCGTTGGCGCCTTGCT11.9607843137254901No Hit
CGTGTGCTCTTCCGATCTGTGCTGGGGTAGACGTGTGCTCTTCCGATCTG11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTTGGTAGATCGGAAGAGCACACGTCTCGCTTTGTAGATCGTAAGAGA11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACACAAAGATCTCTTAGCATAAAACACATTTTTTTCCCATTTTCCTTGGT11.9607843137254901No Hit
GACGTGTGCTCTTCCGATCTCACTGGGGAGACGTGTGCTCTTCCGATCTC11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTATCAACTACTAACCTTCCCACAGCTTCAATCACCTTTATTATTCTAA11.9607843137254901No Hit
GAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCG11.9607843137254901No Hit
CTATAAGATCTTGCTTCGTGGATTCGATGATCTCAGGGCTTTCTTCTTAA11.9607843137254901No Hit
GACCAGGAGCGTATCGCCGACCGAAGGGACAAGCCGACCAATGAACACCA11.9607843137254901No Hit
AACCAGAACTGGTGCGACATGGAACTCGCTACCATTCATCATCATCATGC11.9607843137254901No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG11.9607843137254901No Hit
GACGTGTGCTCTTCCGATCTCTTCAGGGAGACGTGTGCTCTTCCGATCTC11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTAGATAGATCGGAAGAGCACACGTCTCCCTTAAGAGATCGGAAGAGC11.9607843137254901Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTTGATCCTGAGTAACGTGCTGAATATACACCCGGTTCATTATTGATTG11.9607843137254901No Hit
TAAAATACCTAGGTTAAATTTAACAAAATATATTCAAGACCTCTACACTG11.9607843137254901No Hit
CAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGT11.9607843137254901No Hit
ACGCAATCTCCACCTCAATTCGATCGATTTCCTATGCAACATATACAAAG11.9607843137254901No Hit
GGCAAGGAAATACCAGACTAGGTCAATCGCAGGTTACTAAGTGGCGAAAA11.9607843137254901No Hit
CTTCCGATCTGGGCGGGGGGAACTATAGGCCAAGTAAAAGGAGAGAGATT11.9607843137254901No Hit
CCACATGATCCAGCAATCCCACTGTTGGGTATATACCCCTGTCTCTTATA11.9607843137254901No Hit
ACAAACAACTGAAGCGTTTTCAGGTATGTGATCCACATCATCAGAAGGAG11.9607843137254901No Hit
CTCTGAGAGATACAACTCTCCTTTGAAACCAATCTCGGTTGAGCTGTCGG11.9607843137254901No Hit
CTCGCGAGGAGCAACACAGAATTCTGAAGATAAACTGGCTGTCCTGAGCG11.9607843137254901No Hit
GTATAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT11.9607843137254901No Hit
CTTCCGATCTCCTCTGGGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGT11.9607843137254901No Hit
GGAATATATTGGCATTCGAAAATGCTATAAAATCGGGCAAACTATTTAAT11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTAGA50.0145.05
CCTTGGT50.0145.02
CTTGGTA50.0145.03
GGTAGAT50.0145.06
TTGGTAG50.0145.04
CCCTTGG50.0145.01
AGATCGG50.0145.09
GTAGATC100.072.57
TAGATCG100.072.58
GGGGGGG400.018.125140-144
Produced by FastQC (version 0.11.5)