FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6F10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6F10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTTACTCTAGGCTACTACTCACTCACGCCTATCAGTTGTTTGATTTC23.8461538461538463No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT11.9230769230769231No Hit
GTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGG11.9230769230769231No Hit
AGATATCGAAGATTGTAGCGGATTGGTTTGGACCGGCATCGAAAGAGTTG11.9230769230769231No Hit
CTCTCCACAAGCTCTAAAAGCAAGTCCCCATCCGTTTACCGAGCCAGCTC11.9230769230769231No Hit
AGCCCATGCCGGTCGAAGATACCAGGCCGCTGCGACTGGTTATTAAAAAC11.9230769230769231No Hit
GATCTGACACGGGTAGGTAGTGACAATAAATAACAATACAGGGCTCTTTC11.9230769230769231No Hit
TTCCAGAGCAGGAGACCCCTAAGGTTGCAACAGAGGAATCATCGGCGCCA11.9230769230769231No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT11.9230769230769231No Hit
CCCATACCCATCGGTTGGTTTTGGTTCATTCCGGCCCCCATACCCATACC11.9230769230769231No Hit
ATTCAAACCTTGATGGCAACAGATCTCACGTCGTGAATACAGCATGGGCT11.9230769230769231No Hit
GCATCAATCTCACCAAGATTACATGGACAAGGCCGATAAAGCTCGAAAAG11.9230769230769231No Hit
GGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCA11.9230769230769231No Hit
CGTATTAGCTCTAGAATTACTACGGTTATCCGAGTAGTAGTTACCATCAA11.9230769230769231No Hit
GTTCTTGGACCGGGTGTGCAAGTCACCGAGCCTGATCTTCACAAACTTCC11.9230769230769231No Hit
GGCGGGTCGCCAGCGGAGTAGACCCGTGCTTCCGTTGCACCTCTTGCTAG11.9230769230769231No Hit
GGATCTTATTGGAATCCCGAGTGCGTTGGATACCATTGGTAAAGTCCATT11.9230769230769231No Hit
TATAAATACATCATAGATGGTGAATGGACACACAATGAGGCCGAACCGTT11.9230769230769231No Hit
CTCATAGTCGTTCCTCTCTGCAAAGGCAATTTAGTCATGGGCATAGTAGC11.9230769230769231No Hit
GCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCACACGTCGCGTGGTGT11.9230769230769231No Hit
GCAGTGGCCATGAAAGCTAAAACGCAGAAGGTCGATAATCCATTTGAATC11.9230769230769231No Hit
CTACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAAC11.9230769230769231No Hit
GCCCAGAATAAAGCCATGGCTTTCTCGTAAGGATCTTGAGGAAGAATTGG11.9230769230769231No Hit
GCATGAACTTCCCGCAGTCGATCGTGTTCGACAGTGCGTTCGTGCTGCCC11.9230769230769231No Hit
CCTCTAAAGGCAATGTTCTTCGGTGTGATGTGACGGGGAAGGTTTTAATG11.9230769230769231No Hit
CGTCTCCCTTTGCAGATCGGAAGAGCACACGTCTCCCTTAAGAGATCGGA11.9230769230769231Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAG11.9230769230769231No Hit
GTCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCT11.9230769230769231No Hit
TTGCTGGAGCATACAAGGCTTTGGACGAAAAATACAGCGAAGCCTTTCTT11.9230769230769231No Hit
TCCTCGTAGACGTAGAAGGTTTAGGAGGACAGAGTTTGGTGTGGATAATG11.9230769230769231No Hit
GAGCGAACCGGGAAGAGCACAGCTTGAAAATAGGACGTCTTCGGCGTTAG11.9230769230769231No Hit
AGTTCTGAGCTGAGTGTTCGACGCCCGGGGAAAACTCCCGAGAGAGCCGT11.9230769230769231No Hit
CCTGACAATGTCCTCCGCCCGGATCGACCCGCCGAAGCGAGCCTTGGGAC11.9230769230769231No Hit
AATCTACCCATTGATGATTTCCTCAAGAAGACTGTTACAGAGAGCGAAGA11.9230769230769231No Hit
GTCCGAATTCGTTCCTACTTACACATGCATGGCTTAATCTTTGAGACAAG11.9230769230769231No Hit
GAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATCTGTCTCTTA11.9230769230769231No Hit
CTCCCATTGGAGTCACATCCTTCTCAGACATGTCATATTCAGTCATGGCT11.9230769230769231No Hit
GGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAG11.9230769230769231No Hit
TTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAATGCCCCCC11.9230769230769231No Hit
CCAAGATGACCTGGCAGTTGCTCTTTAATCTTTTCTGCTATACATTTTTT11.9230769230769231No Hit
TCCCACACGGAGGAACAATGCGACGGAGGCGCAATCTCAGCGGAGCGTCG11.9230769230769231No Hit
ACAAGGCATTGTCGACATCGAATACCAAAGAGTTCCTTGCGATTACGGCA11.9230769230769231No Hit
AGATTATAGATGTTGAATGATGTAAAGTAAAGGTGTTTTCCTACTTTGAT11.9230769230769231No Hit
GGGCTAAGGTTATTGACCTCCAGAAGCAGTAGTGACCCTCTCTCTTTCTT11.9230769230769231No Hit
ATAGTATACAACTTCTCATTCGTCGCTCACGTAATGATCTTCTTTAATAC11.9230769230769231No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG11.9230769230769231No Hit
CAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGCTC11.9230769230769231No Hit
CCAAAACTTCTTTATTGAACAAATTCCCAAGGAGATCAATCACGCACGGC11.9230769230769231No Hit
GGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCGCTCAC11.9230769230769231No Hit
CTATTACACCTAAGGCGAACTTCAATTCACCAAACCAAGGTTCATCTCGT11.9230769230769231No Hit
CAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGA11.9230769230769231No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAAC50.0145.07
GTTCCAT50.0145.01
CCATGTG50.0145.04
ATGTGAA50.0145.06
TTCCATG50.0145.02
TCCATGT50.0145.03
GTGAACG50.0145.08
TGAACGG50.0145.09
GGAGTCA50.0145.0145
CATGTGA50.0145.05
Produced by FastQC (version 0.11.5)