FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6E6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6E6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGAT26.451612903225806No Hit
TGGTCAGGGTGTCGGCCAGCTCTTGCGTCGTCAGTGGTGGATTGCAGAAT13.225806451612903No Hit
TCATTGGAGTCGTCAAGCGATGATTCCGTAAGACAACCGAGGAATGAAGA13.225806451612903No Hit
TGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGA13.225806451612903No Hit
ACTTGCATAAACCCTAATCCCCCAAATCGGATTAACCTCCGAAACCGTAG13.225806451612903No Hit
CATACAGATATGAAGATTAAGATAGAAAACGAAGAAGAAGAGGGACAAAT13.225806451612903No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCT13.225806451612903No Hit
TCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCTT13.225806451612903No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAGTG13.225806451612903No Hit
TGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGC13.225806451612903No Hit
TGCAAGCTAAGGGCCCACATGCGCATTAGGAAGATGGAGTAGAGCTACCA13.225806451612903No Hit
ATGGAGAACTGGTTCAACAAAGGAAGCAGGAAGATAAAACCGGAAGGGAA13.225806451612903No Hit
GTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGA13.225806451612903No Hit
GAAGATGGAGTGTGTATTCGGTCTTGTTGGGAATGGATTCGCCATCGTTG13.225806451612903No Hit
GTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGAC13.225806451612903No Hit
CCATAGGAACGAATCTACAGGGCTATCACCTTCTTTGGCCAGATCTTCCA13.225806451612903No Hit
GATCGGAAGAGCACACGTCTCCCTACTGAGATCGGAAGAGCACACGTCTC13.225806451612903Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACATAATACTGTTACATTGGTTGCTAGGGCTCGTGCTCGTGGCAATGATC13.225806451612903No Hit
AAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTC13.225806451612903No Hit
GGGCATCACGGAACTGTGAAGAAATGTCTTGAAAGTTATTCTCTGACTGA13.225806451612903No Hit
CTTCCGATCTCGGCCGGGGGTGGGATAATGTATGATGTACCTATTATAAC13.225806451612903No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13.225806451612903No Hit
GCCCACGTCTCCCTGTGTAGATCGGAAGAGCACACCTCTCCCTCTGTAGA13.225806451612903Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTATGGAA13.225806451612903No Hit
ATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGG13.225806451612903No Hit
GCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTT13.225806451612903No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC13.225806451612903No Hit
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATC13.225806451612903No Hit
GCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCATCCAT13.225806451612903No Hit
GTGTGGAAATCACTAGTGGGATCGAACCAGAGTTTGAATTGTTGTTCTCT13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)