FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6E2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6E2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAA25.555555555555555No Hit
CCTCCACGCAGACGCAACACCAAGTGAAGGGTCGACTCCTTCTGGATGTT25.555555555555555No Hit
ATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTC12.7777777777777777No Hit
TGGTGCGACAGGGTCCGGGCACGACGGGGCTCTCACCCTCTCTGGCGCCC12.7777777777777777No Hit
TCCCCTTTCAGATCGGAAGAGCACACGTCTCCCTTTTCAGATCGGAAGAC12.7777777777777777Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACATAAATACCCATACTAGCAATGAAAGGCATCTCCTTGGCTCTCTGATC12.7777777777777777No Hit
ATCTTATATATCCCTGATTAATTTCATTTCTAGCTAATCATTATTATTAC12.7777777777777777No Hit
GGTAAAGCCAATGATTAGAGGCATTGGGGGCGCAACGCCCTAGACCTATT12.7777777777777777No Hit
GTTGTACCCCGCCTCCGATTCACGGAGTAAGTAAAATAACGTTAAAAGTA12.7777777777777777No Hit
TTTTAAACCTTCCGAGTTTTGTTGATGTTATTCCGAGAATTAGCAAACCG12.7777777777777777No Hit
TTCTCATTTCCTCTGACCTCTTTTGAAACATCTCTCCTTGCTCGCTCTAT12.7777777777777777No Hit
CTTTCCAGGGTCTGTGGAGGAAGTTTCAGATGGGCCTTGAAGGGAAGCTG12.7777777777777777No Hit
GTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGC12.7777777777777777No Hit
ATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAAT12.7777777777777777No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG12.7777777777777777No Hit
GATATACCTTGGTTATATTTCATCTCTTCAACAAGTTTCGTAATGAGCAT12.7777777777777777No Hit
GCACAAACATCGGCGCTTGTTCACCTCTCGTCATCGCATTTGTTGCCTTG12.7777777777777777No Hit
GTCAGCTCAGAACTGGTACGGACAAGGGGAATCAGACTGTTTAATTAAAA12.7777777777777777No Hit
CTTCCGATCTGACCGGGGTCCCGACAGGCATGCTCACACTCGAACCCTTC12.7777777777777777No Hit
TCTACGATCTTGCTTTTCTCGCTAACATAGATACCATCAAGAAACTTCCT12.7777777777777777No Hit
GAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTAC12.7777777777777777No Hit
CGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAG12.7777777777777777No Hit
GTAAGTAACTATGCACGTCTTGACGGTACCTAATCAGAAAGCCACGGCTA12.7777777777777777No Hit
GGATTTAGATTGTACTCATTCCAATTACCAGACTCGAAAGAGCCCGGTAT12.7777777777777777No Hit
CTTCCGATCTACTGAGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGA12.7777777777777777No Hit
CGAGCTGGGCAATGGGCTCCTGGGTCTCCTTCGGCAATAGATGCCGTCAG12.7777777777777777No Hit
CTCTCATGCATATTCACAATCTCCACTTCCATCTTATACAATTCCAGTTC12.7777777777777777No Hit
CAGTATCACGTTCAAGTTTATCCTTCCAGTCGACCAGCGAAGCTTCATCC12.7777777777777777No Hit
CCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTTACCACCATGGTA12.7777777777777777No Hit
ATAGTGTTCTAAACGAAATGCGGGAAAGGATTGAGGAATTAGTTGAAGAG12.7777777777777777No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT12.7777777777777777No Hit
CTTCCGATCTGCTGGGGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCA12.7777777777777777No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC12.7777777777777777No Hit
CCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTATGGAAG12.7777777777777777No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)