FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6D9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6D9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTACATGATCCTGGCAGGTGAGAGGAAGAAATAGAGCAGAATGGTGC13.4482758620689653No Hit
CTGACACACATAATCATAACTAATAACAAGAATGCCAACAAAATACGAAA13.4482758620689653No Hit
CTCCCGGTGCACATCGGAAGATCACACTTAACAAGGTGCAGCTCCGAAGA13.4482758620689653No Hit
GTGTGACGGCTCCACAAAGCAGCCGACACGTCCAACCTATTTAATGTTTG13.4482758620689653No Hit
GATAAGATCGGAAGAGCACACGACACCCACATAAGATCCCAAGAGCACTA13.4482758620689653No Hit
CATATATACACCTAACACTGGAGCTTCCAAATTTATAAAACAAATACAAA13.4482758620689653No Hit
CACCTCTCCCAGGATAGATCGGAAAAGCACACCACTCCCAGCTTAGAACT13.4482758620689653No Hit
GACCAGGACGCATCCCCCGCCCCCATCCGCTACCCACCCGACAATATCAA13.4482758620689653No Hit
ATCATACGCTTTACCCGAAACAACACCCCACCGACCACCACCAAACCACA13.4482758620689653No Hit
GTGAAAGCGTGGCCTATCGATCCTTTAGACCTACGGAATTAGAAGCTAGA13.4482758620689653No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAAGCATGGCGCT13.4482758620689653No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13.4482758620689653No Hit
TATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCAA13.4482758620689653No Hit
CTTCCGATCTGCTAGGGGATGGCAGAGCCCGGTAATCGCATAAAACTTAA13.4482758620689653No Hit
GTGCCGAACTGCGCGCTAACCTAGAACCAACAAAGGTTGTTGGTCGATTA13.4482758620689653No Hit
CATGTAAGCAATAAGAGGAGTTTAACAACACATAATCATGATCCCCTAAC13.4482758620689653No Hit
CAATTTCAAGAAATCAAGGAGGAAGGACTCTTCCCTGACTCATTCTAAGA13.4482758620689653No Hit
CCCCACTCAGATCGGAAGAGCACACGTCTCCCAGTGAAGATCGGAAGAGC13.4482758620689653Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAAAGAATAAACACAATCCCGGCCCAAAGCCCGCTACATATTCACAA13.4482758620689653No Hit
CTTCCGATCTGGCGGGGACTTTAAATAGGTAGGACGAGTCGGCTGCTTTG13.4482758620689653No Hit
CTACCCATCATTTTATTTATATATTTTTTTTTTTTTTACTGCCAAACCCC13.4482758620689653No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGACGGTGCAGATCTTGGTGG13.4482758620689653No Hit
CATCTCGCGCGCACACAGATAAACCACCAACGCGTCCAGAGACACCCACC13.4482758620689653No Hit
CACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCACTGCAAGCTCCGC13.4482758620689653No Hit
GAAGAGCAAAAGTCACCCACACCAGATCTGAAGAGCACAAGTCACCCAGT13.4482758620689653No Hit
CTATCTCATCGTCATCCACCCCCCAACTTTCCTACTTGAAAAATCAAAAC13.4482758620689653No Hit
AAACCCACTGGAAGAAGGTGAAGAAGCCCAGGATGATGACCACGTCGTGC13.4482758620689653No Hit
CAAGAGCACACGACACCCAGCACAGATCACAAGAGCACACCTCACCCACC13.4482758620689653No Hit
CGGACAGAGAAAGAAGAACATTATATTCACTGAATCAGATAGTTTGTTGC13.4482758620689653No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)