FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6D8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6D8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAACATCAACACCTTCCCGAAACACTTCACCAACAACTCATTAAAAAA11.7857142857142856No Hit
CCCGTACGCTGACGAGCAAAAGCAGCAATCCGCCCGAGGACGGGATAGAT11.7857142857142856No Hit
AGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCTT11.7857142857142856No Hit
CTACTAGATGGTTCTATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA11.7857142857142856No Hit
AAGAATGCCAACCCGGTTCGGAATCACGTAGCTGAGTCAGCACAAGCCCT11.7857142857142856No Hit
ACTTAAAACTTGGACCAGAGGCATTATAAGAAAATGAATTAGTTCTGTCT11.7857142857142856No Hit
TTTTAAAAAATAAAAAAAGACCAAAAAAACAAAATTAAACATAAATCAAA11.7857142857142856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.7857142857142856No Hit
TCTAAGACTGCTCCATTCTTTCTCAATGACATACTTGTTTTCAAATACAA11.7857142857142856No Hit
CCGCGGGACGATGCATTCTCGTTAGGAAATCGTCTGATTCCTGATATCTG11.7857142857142856No Hit
TCCGGAGGACCGAGAGCCGCTCACGCCCCGACGTACTCATAACCGCATCA11.7857142857142856No Hit
GTCACACCCAATACGTCCACTTAGCGTGTTGGCAGACAGCAGTGATATTG11.7857142857142856No Hit
CTATTAAACGTTGTTAAGGATGTGACTTCTTCTCTTGATCTTCACCTTAA11.7857142857142856No Hit
GTACTGAGAATATTTGCAAGATCAATGAGAACCTACAGCTCAGCTGCACA11.7857142857142856No Hit
CACCCGTTGACTCGCACACATGTCAGACTCCTTGGTCCGTGTTTCAAGAC11.7857142857142856No Hit
ATAGAGCACTGAAGGATTTGGCCTTCGAGAAACCAGCTTCGCTCATGACA11.7857142857142856No Hit
ACGTCTCCCAGTTGAGATCGGAAGAGAACACCTCTCACAGTTGAGATCGG11.7857142857142856No Hit
ACCAATACCCGGCCGTCGGGGCAAGAGCAATGGCTCGATTTTTATGTTTG11.7857142857142856No Hit
ATCAAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGATTGAGCTCG11.7857142857142856No Hit
GCATTGCGATGGTCCCTGCGGATGCTAACTCAATGTGATTTCTGCACAGT11.7857142857142856No Hit
CTTTTAACGTTATATTACTTACACCGTGAAACGGAGGCGGGGAACAACCC11.7857142857142856No Hit
CTTCCGATCTGATCAGGGCTGACACTGTCGCTGTCCAAGATCTCGAAGGT11.7857142857142856No Hit
ATAGAGAACCTGAGATCCGATGCGAACAATCAGTCGAATGAGAAGTCAAG11.7857142857142856No Hit
CTCATCACAACAGCACGGGCACCAAGTGACAATAAAAAACAAAACCGGCC11.7857142857142856No Hit
AAGATCGAAAGCTAACGTATACCTAAAGAGAACCATTTTATAACATTAAA11.7857142857142856No Hit
GATTAACAGGGACAGTCGGGGCCATTCGTAATTCATAGTCAGACGTGAAA11.7857142857142856No Hit
GAACTAACCAGGGGGTGATCAACTTGCTATAGGTAAGGAAACCGGTCGCA11.7857142857142856No Hit
GTTAAGGGATTTAGATTGTACTCATTCCAATTACCAGACTCGAAAGAGCC11.7857142857142856No Hit
ATGGTAACTACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGC11.7857142857142856No Hit
CAAAAACATAGAAACAAATACAGTAATTTGAAGTCTCAAGACGAAAGTAG11.7857142857142856No Hit
GCTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCA11.7857142857142856No Hit
CGGCAGATCCTGAAGCTTCACCTCCGTCCCTTTCTTCAAACAACCCCACC11.7857142857142856No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACACCCTCAGGTCGA11.7857142857142856No Hit
TTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTA11.7857142857142856No Hit
GTCGTACTCATAACCGCATCAGGCTGTCTCTTATACACATCTCCGAGCCC11.7857142857142856No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTAAGGTTGAGT11.7857142857142856No Hit
GGGTTACGGTGCCCAACTGCTCGCTAACCTAGAACCAACAAAGGGTGTTG11.7857142857142856No Hit
ATTCGAAAGAGTACAATCTAAATCCCTTAACGAGGATCCATTCGAGGGCA11.7857142857142856No Hit
GAAAAACAAAAATCAAACATGCAAAAATCCCACTTAAAACTCAAAATACA11.7857142857142856No Hit
CAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAG11.7857142857142856No Hit
GATATGACTACGACCGGGCGTGACCGGCACTCGGTCCTCCGGATTTTCAA11.7857142857142856No Hit
GGTATAAGCCTGCCTGACTGTGAGACCGACTGGTCGAACAGAGACGAAAG11.7857142857142856No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG11.7857142857142856No Hit
GGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAG11.7857142857142856No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAAGTCAGACGA11.7857142857142856No Hit
GAACATAACTTAAAAGATAACGCAGGTGTCCTAATAAGAGCAAAACAAGA11.7857142857142856No Hit
CCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGAGCGGCACTC11.7857142857142856No Hit
CACAAACCAACACCCCAAAATAGCCCCCAAACTTCCGCACAAGCGCTGCC11.7857142857142856No Hit
CATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGA11.7857142857142856No Hit
AACAACGGAACCCTTGATGTAGCTGTCGCGGTAGAATTGACTCAACGACT11.7857142857142856No Hit
CTTCCGATCTCGTCAGGGAGACGTGTTCTCATACGATCACGTCAACTAGT11.7857142857142856No Hit
CAAAAGGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCA11.7857142857142856No Hit
CCTTTTATCTAATAAATGCGTCCCTTCCATAAGTCGGGGTTTGTTGCACG11.7857142857142856No Hit
GATTAACAGGGACAGTCGGGGGCATTCCTATTTTCATAGTCAGAGCTGAA11.7857142857142856No Hit
TCTCTATGCATCTCCCTTTCTCTACCTTTTCCAGTGTCAGCACCTCGATT11.7857142857142856No Hit
CCAATACATCAACGATTCGATGAAGCCTGTTCACTGCTGTTTTTGTATCG11.7857142857142856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGG50.0145.07
ACATGCT50.0145.0145
TAGCCTT50.0145.05
CCTTGGA50.0145.08
CTTGGAG50.0145.09
TTTAGCC50.0145.03
TATTTAG50.0145.01
TTAGCCT50.0145.04
ATTTAGC50.0145.02
AGCCTTG50.0145.06
Produced by FastQC (version 0.11.5)