FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6D5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6D5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCAGGGGAGTAGGAAAAAACAAAACCAAAGAAGATCTACAGATAAAGA10.5847953216374269No Hit
CATATCGGTCCAAAGTCACATACATACTTCCTCCACCAGATGCAATCAGA10.5847953216374269No Hit
GGTTATGGTTGAGACAAGGACGGTAACTGATCCTCTTCGACCCAACAACT10.5847953216374269No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGCCAGCAGGACGGTGGTC10.5847953216374269No Hit
AGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCG10.5847953216374269No Hit
TCCCTGTATCAGCTTTGAAATCAATCATTCCTTGTAACACTAAGATTTCG10.5847953216374269No Hit
GTACCACCACCAACACAACGCTGTATCCAGACCTAAGATACACGTCACTA10.5847953216374269No Hit
TAAACCAATCACGACTTACCTATCCCAAAAACGCACGACCGTACTAAACC10.5847953216374269No Hit
CAAAAATCCAGAAAAAAAACAATCACCGATCGATCATAGCCTCATCCACA10.5847953216374269No Hit
CCCAATTTACGTTCCTAATGGGTCCGGTTTACACTGCCTTAAAAAACGAG10.5847953216374269No Hit
CCTCAAAGGGATCCATTTTGTTGCTCACCATAATGCCCAGAGGATTAGCA10.5847953216374269No Hit
CAATATGATCAATGCATACAAAACGGAGACAAAGAACATCGATGCCGAAA10.5847953216374269No Hit
ACGAAGGGGCTTGTCTGATGGCCTCATGCGCTCGTTGATCTGCTCAAGAT10.5847953216374269No Hit
CATCAAGACTTTAATCACAACATCCTCTTGGCAGCCTCGAAGCCTTGCAC10.5847953216374269No Hit
CACCAAAAGCCTAAATTTAACTAGATGCAGATAAAAAGCACCTACTACAC10.5847953216374269No Hit
CTTAATCCCTAACGTCTTCGGTTTTCCGATAGGTTTATCAGCTCCAGTCG10.5847953216374269No Hit
TATCTAATCCTTGTCTTAGTACCATCATGTTTTCCAGACAATTATCAATC10.5847953216374269No Hit
CTTTTGGTGTGCATTGGTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCT10.5847953216374269No Hit
GTGTAACAACTCACCTGCCGAATCAACTACCCCCGAAAATCCATGGCGCT10.5847953216374269No Hit
AAGGCAAGCTTAGAGGCGTTAGGGTCAAACTGTTGACCTTAAACTGGAAG10.5847953216374269No Hit
GTGCAAAGACAATGTTGAGCGTTAAAGCCAATAGTGGTGGATCGTAACTT10.5847953216374269No Hit
CGGCGCAACGCTCAGCTCTCTCGCGACCCCTACTCTAATCAAAAGACTAA10.5847953216374269No Hit
GTCAAAGACTCTTCCTTTATCATTCTGTACACAGATCCCGAGTTTTTAAT10.5847953216374269No Hit
CCGCTGGCACCTTATCAGAAATCAAACTATTTGGCATCCGGGCAGAGTAT10.5847953216374269No Hit
GTTAAACAACAAAAGAGATAATGCCAAAATTTGCAAACATTCTAACACAA10.5847953216374269No Hit
ACACGGACCAAGGAGTCTGACATGTGTGCGAGTAACCGGGTGACTAACCC10.5847953216374269No Hit
GGGGATGCAAGCGGTGGAAGCGGCGGCGAAGAGTTTCGTCTTTGGGTATA10.5847953216374269No Hit
GAAACATGGGCGCATGGAACTCCAGACGGGGTAAGTGAGCCGGGTAATTG10.5847953216374269No Hit
CTTTTTGATCAAAACCCTCGACAGTGACGTCACCGGAGATTTTCTCTCCG10.5847953216374269No Hit
ACCTTGGGAAGGGTCCCCCGGTCCGCCATTGGTGAGCATTGGACCGCTTG10.5847953216374269No Hit
CTCACGCGCTGACCCGTCTTTGCAGCCGCCGTTGCTCCTGAAAATAGCTC10.5847953216374269No Hit
AAGCGCTATTGTCGAAACTGTTTTTTCCAAGTCCCTGATCATCAGCAAGA10.5847953216374269No Hit
CTAACGAACATCAAACGGAACATAATGTTATACATCATCAATAGCAAAAG10.5847953216374269No Hit
AAAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTA10.5847953216374269No Hit
CCTCATACATCGATACAAAAAGCTGATAAAGTTAACAACATCACACGCAC10.5847953216374269No Hit
TCCTCACCCAGTTCGTCAGTGGTTTTTATACAAATAGGGTCTACAGGGGA10.5847953216374269No Hit
ATCACAAGCTTCCCCATTTTTCCAGCGCCAATTACACACATCCTAGCGGA10.5847953216374269No Hit
ACCTGAATCAGATGACGCAATTGCACAGTGGTGCAGAGATCAGTTTGTGG10.5847953216374269No Hit
CACAAATACAACCGACAAATCAACAGTCTCAGAATTAATATTACACGTCA10.5847953216374269No Hit
GCATGGAGACCTTCCACCGCTACCACAAGTACTAGAACTATACTCATCCT10.5847953216374269No Hit
CATCAATAACTTCAAAGGTATTGTTGTTCTTCTGCAACCAAGTAAGAGTG10.5847953216374269No Hit
GAACTTGGCACTGTTTTTCACTTCGTTTTTCAAGTTACATATAAAAATCC10.5847953216374269No Hit
AACTAGACGAGTGCTCATCCCAACTAGAACTGTACTCTAACCATCAAGAC10.5847953216374269No Hit
CACATGCATGGCTTAATCTTTGAGACAAGCATATGACTACTGGCAGGATC10.5847953216374269No Hit
CATACTACCGTGGGTTTTTTAAACCTTCCGAGTTTTGTTGATGTTATTCC10.5847953216374269No Hit
GGTACAAAGAGAAAGGTATTGAGCTGATTATGGGCACAGAGATTGTCAAA10.5847953216374269No Hit
TAAATATATAAAGGGTCGGTGGCTCCTTCGGCTCCCAAGTTCCTTCGTTT10.5847953216374269No Hit
GCAATATAGCAAATGAGTAGCATATATATATGTCTATCTATACAAAACCA10.5847953216374269No Hit
AAACTCCCCCCCGAACCCAAAAACTTTGATATCACATAAGCTCCCAGCGC10.5847953216374269No Hit
AGTTAACACATTCCTCGACCAGAAGAAAACGGAGTTGAGGTCAAAGTCAT10.5847953216374269No Hit
GAGAGCAAGAGACGACATCGAAGGATCAAAAAGCAACGGCGCTATGAGCG10.5847953216374269No Hit
CTTCCACAGATGCCAACACTAACTGGCTGAGCCGCAACAGCCACCATTAA10.5847953216374269No Hit
CATCATCAACAATCAGATCAGGACCACCACCTGGACCCCAATCTAGAGCA10.5847953216374269No Hit
CTCAAATATGATCCCACAAAGAAAAAAGAAAATTGTGAAAGGGCTACGAA10.5847953216374269No Hit
GAGGTGGCGTCATCGACGAGGATGCACTCGTTAGAGCTCTCGATGCCGGA10.5847953216374269No Hit
ACTAAAAACTATACAGTCAGAGGTACAATTCCTCTTCTTAACACCAGCAG10.5847953216374269No Hit
GCCTCATGAGGAAAGATACTAACCGGTATGATTATTCTGTATCTTTTCTT10.5847953216374269No Hit
AAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAG10.5847953216374269No Hit
GCAATGGCATCAACAAGAAGTTTAACATCCATCGCCAAACACTTAACCAG10.5847953216374269No Hit
TAAATAGAGAAAGCTGATACGATCCAATGAAACACCGCTGCGGATGCGCC10.5847953216374269No Hit
CCCGTTGACTCGCACACATGTCAGACTCCTTGGTCCGTGTTTCAAGACGG10.5847953216374269No Hit
GGCGTGGACCGACGCGGATTACGGTGGCGGCCTAAGCCCGGGCTTTTGAT10.5847953216374269No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCCGATGGGGGCCGGCGA10.5847953216374269No Hit
TATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGC10.5847953216374269No Hit
GAAATGTGGAGATTGGTTTAGAAGAGAAACCCTGATGCCAAAGCAGCAAC10.5847953216374269No Hit
CTAAAAAAACAATCGTGGATTATCAGCTCACTGACGATCAGATCTTAGAA10.5847953216374269No Hit
TACTAAGGGAGAAGAAAGGCTAAGGCCCAATTGTTGTTGAAAAAGCCCAA10.5847953216374269No Hit
TCCCATCATTGATCTCAACTTTGATGCGTTGGTGCATAATCTGTTTCTTG10.5847953216374269No Hit
GTGTGATAATGGGCATTTGGCTTGCGGTTCTTGCTGTCCCAAACTGAGTA10.5847953216374269No Hit
CTAAGAAAAAAATCCATACGACGACATCAAGCACCAAGACCGCATTATCC10.5847953216374269No Hit
TTGCTGAACTCAAGCTTGTCCTCGTTCTTGACAGTGGTCAAGCACAAGGC10.5847953216374269No Hit
CCGTCCTGCTGTCTTAACCGACCAACACCCTTTGTGGGTTCTAGGTTAGC10.5847953216374269No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA10.5847953216374269No Hit
CATTAATCAACAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCC10.5847953216374269No Hit
TCCTAACTCCAGGCAATCCATTAAGCGTCTCTAGTACACTCTTAACGCTA10.5847953216374269No Hit
CAAGAAGAGAATCAACGAGAAAAGGACAGCAAAAAGCAGAAGAATCCACA10.5847953216374269No Hit
GCTAAAGGCAAGATCGAAAGAATCAGACTTCTAAACGATGCACTCAACAC10.5847953216374269No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGCCGTTGCTTTTTG10.5847953216374269No Hit
AAGAAAAGGTGAAAAACAGAGCCTATAGAGTTCTTGTCCCGGCAAGTCCA10.5847953216374269No Hit
AAACGTATCCCTCCTATGAATAATATAAACCTTAGATCCATACTTAGTCA10.5847953216374269No Hit
ACTAAAAGCGCAGTCAAAAAGGACACGATCACGACTTACATCTGCAGTAG10.5847953216374269No Hit
AGTAGCTGGTTCCCTCCGAACATTCCCTCAGGAAAGCTGGAGCTCCCACG10.5847953216374269No Hit
CAAGAAGATTACACCTTAGGAAAGAGCTTACACCTAATCGCTTAACCCGC10.5847953216374269No Hit
AAGCAGGAACAATCGTCGGAGGAGTTCGCGATATTGATGCAAATGCTAAT10.5847953216374269No Hit
AGACATTGGAGAGAGACAGAGAGTGTGAATTGCCATTACCATCACACACT10.5847953216374269No Hit
AACAAAGACAATACCACGACCGCCAAGCGCCCCCAATCCCTCTAATCATT10.5847953216374269No Hit
GTATAGTTCCTTGGATGTTGTTTTTGTAGAGCTCTAGATACTGTAAATGT10.5847953216374269No Hit
AACTTCTACTTCATCACTTATTTTACCATCAATAGATAGCTTGAATCTGA10.5847953216374269No Hit
ATTTTGCCGACTTCCCCTGAAAATCTGGAGGACCGAGTGCCGCTCACGCC10.5847953216374269No Hit
TCTGGCGAATCGCGACGGGACAAGATCCGAGCCGTGTAGATTAAGAATGG10.5847953216374269No Hit
GCACATCCACCAAAACAACAGTTACAAAGACTGTCTCTTATACACAACTC10.5847953216374269No Hit
GCCTTGCGATTACCACCAGCGGCTAGTCTCCAGAGTTCGTCAAGATCCAT10.5847953216374269No Hit
GCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAG10.5847953216374269No Hit
ACAATAACAGCAAGAGAAGTTGCATCATCGAGATCATGCAACTCAACCAA10.5847953216374269No Hit
CCTCATCTAGTCACCTATACAGATAATCGCAGAATCATCTAAGCTCATGC10.5847953216374269No Hit
ATACCAAGTAAACCAACGCCGTCACAAGGACCGGTTTGCATCTTGAGGCC10.5847953216374269No Hit
TCTCTCATCATTAACCTGATTTCACAATTTCTGACTTTACACAACTCCAT10.5847953216374269No Hit
GGAAAGAAAGGGCGGTAGATGATAATGAAAAAGGGCGCCGCGTCTGTACG10.5847953216374269No Hit
GATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCTCGGGGGT10.5847953216374269No Hit
CCAATAGACAAACCCTAAGCCGCATCCAGCTGAGATCCCCCAGAACCATT10.5847953216374269No Hit
CTATTTACCCAAGGACCGAATATACCGCCCCAAAGCGGCTCCAATCCCAA10.5847953216374269No Hit
GTGCTCTTCCGATCTCGCACGGGGCTCCATTTATTTTTGCTCATCTTCTT10.5847953216374269No Hit
TGGTAGGCCACTATCCTACCATCGAAAGTTCATAGGGCAGAAATTTGAAT10.5847953216374269No Hit
CTTAAGCGCCAACCATATACCGCGCTAGTAGAAACGGCACCTCAGTTGTT10.5847953216374269No Hit
CTTTGAGGGTTCTACCTTAGCGCGCAGTTGGGCACCGTAACCCGCCTTCC10.5847953216374269No Hit
TATACACACCGCAAACAACCAAATAGCCGCCGAGAAACAATCAATGCAAG10.5847953216374269No Hit
AACGAAATGTGAACCCTTGTCTCGCCTAGGTTGGAAGGGCGTGCTTCGTT10.5847953216374269No Hit
CAACTAACCCTAAAACTCTCTGATAATCCCTTGCCCCGAAAGTTACCTAC10.5847953216374269No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10.5847953216374269No Hit
AAATACTTCAATGATCAATAACACATCCAACGCCAAACCAGCACACACAG10.5847953216374269No Hit
CCTATACGGTTTGCTGTGTTCATGGTGCATTTGGCTACTATCCCCATAAC10.5847953216374269No Hit
GCCTTCACCTACAGCTGCAACAATGGTGCTGAAATTATCATCTGCCAAAA10.5847953216374269No Hit
GAACAATGTAGGAAAGTTAAGTAGTCAAAATGTAACCGTAAGTTCTTGAA10.5847953216374269No Hit
TTCTAGACCACCAGAAACTGGAAGAGTCACTGTTCTCACAACATTCCGGA10.5847953216374269No Hit
AAAAGAAAGTGGCTCGAAGCTACAGATCGCTGTAGTATGACTTAACGCCT10.5847953216374269No Hit
TTCATAGAGACAGAAAGATCTAAGTTACCGCAATGAACGAGCAGTCAGTT10.5847953216374269No Hit
CAATTAACCTTGACCTAAAGAGAGGAGCAAACGCAAGCTTCTTGAAAACT10.5847953216374269No Hit
AAAGAATATATATCTTACAAGAATCATCATCAAGACAAATATAATGACAA10.5847953216374269No Hit
CGACAGAATGATGACTTCCGTCGTATACCGACAACACGACAATCTACGTC10.5847953216374269No Hit
CAGTAAGATTATTTCGAGTAACCAACATAACATAAGCTCTTGTTTATATG10.5847953216374269No Hit
TCTCAGAGGTGGTATGCAAATCTTTGTCAAGACCCTCACTGGTAAAACAA10.5847953216374269No Hit
TATAAAAATAATAATCACATTGCTACTCCTGATGAGGTCGATGCATTGTT10.5847953216374269No Hit
ACTAAAGCCAATGCCAGCAAACATGGCTCGGTACATGAAAGACTTCGCCG10.5847953216374269No Hit
GAGTAGACCGAAAGCTGAAAGATAAGGAGACCAAAGCCAGAAAAAGTCAA10.5847953216374269No Hit
AGCATGAATCGCACAGAGATTAGTATCTTCAAAGAGCACAACAAGATAAG10.5847953216374269No Hit
CTCGAAGGCAAGCTAAGACGCGATAGGGTCAAACAGTTGACCTACAACTA10.5847953216374269No Hit
GTAGTGAAAATTCTTTTGGAAGAAGATCAGGCTCAGAACATCCAAAAGTT10.5847953216374269No Hit
CAAAAGAATAACACAAGGCTGAAAAGTCGACGAGTAAGCCCAAACAAACA10.5847953216374269No Hit
TAACTAATAAGAAGACGAGGAAATAGGCTACCAAAACAGAGTCATACATT10.5847953216374269No Hit
TCAGAGTGCTGATAGGAACGTTCCATTCAGAGAGAAGGTTATCTACACTG10.5847953216374269No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGATCCCT10.5847953216374269No Hit
CCATAAGCCACCAAATCAGTTCTTCCCTTTGACACAGACTCATTCCCATC10.5847953216374269No Hit
CTAAACGAATTGATGTTATCAGAGATCAAAGGGAGGGAACGATCCGATTG10.5847953216374269No Hit
GTCAGAATCATCCACGCTAGGTTGCATGCTCAGGCGCTGACATTAGCCGC10.5847953216374269No Hit
CAAGTCTCCCAAGTCACCCATGACACGCATATCACACCAGTCAGATAACC10.5847953216374269No Hit
TTATAACGCAATGGAAGCTACAAAGGCGATAAAGCCAATTCTGGGAGACT10.5847953216374269No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCAATGCTAATGTATCCAG10.5847953216374269No Hit
CAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGA10.5847953216374269No Hit
GGATTTAGATTGTACTCATTCCAATAACCAGACTCGAAAGAGCCCGGTAT10.5847953216374269No Hit
CATTTTACACACTCATAAAATCAAATCTAATAACAATACAACATTACACA10.5847953216374269No Hit
AATCACGACTTAAGCTGGTGACGAACGAAGATTGACAGATCTAGCCGATC10.5847953216374269No Hit
AAAAAAAACATCACGACGATCGATTACTTGCATACACAATCAAAAATACA10.5847953216374269No Hit
AAGAAGAAGACGCAGAGTCAGCATTAACCATCAGTGTATCTAATGGCGCT10.5847953216374269No Hit
GGCATGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCAC10.5847953216374269No Hit
ACTAGGAAAAAACAAACACCAACAACACCCAAACCAAAAGCCAAGCATCA10.5847953216374269No Hit
CAACCGATCAAACTACGGAGACGACTCCACTTCCGTTAAAAAAAAAGAAA10.5847953216374269No Hit
ATGAAAAACAGGGACAGTCCGGCCCATACGAATTTCATAGTCAGAGGTCA10.5847953216374269No Hit
AGCTAACCTTTGAAAAGACGCAGACAACTTAAGATATATCAAGGAACTTG10.5847953216374269No Hit
GTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTT10.5847953216374269No Hit
ACCTATACTCAAACTTTAAATAGGTAGCACGTGTAGCATCATAAGTAGAC10.5847953216374269No Hit
TGTTAATCCAACGGTCTAGTTGGTTAAACCATCTCAATCTACAACTCTTG10.5847953216374269No Hit
GAGTCTGACAAGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAA10.5847953216374269No Hit
GTATTGGTAATACAATACTAATCAGCTTTTATTTGTCTAATTATGCAATC10.5847953216374269No Hit
AGATTGTACTCATTCCAAATACCAGACTCCAAAGAGCCCGGAAATGTAAT10.5847953216374269No Hit
ACTACGAGCATTTTAACTGCAACAACTTAAATATACGCTATTGGAGCTGG10.5847953216374269No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10.5847953216374269No Hit
ACACAGACAAGAGCTTACAAGAGAAAGCCCAACTTGCTCCTCATCCACCT10.5847953216374269No Hit
TTATTTTACTTACTCAGTGATTCGGAGGCGGGGTTCAATCTATGTTTTTC10.5847953216374269No Hit
TCCTAAGGGAACGACTTGAGAAGACAGAAAGGTTCCACATCGTCTCAAAG10.5847953216374269No Hit
CTCTAGTAGTTGCATAGTACTCTTTCCACCCATTGACTGGTGTTATACAA10.5847953216374269No Hit
AAAAAGAAATAACAAGAAAGAACACAAACACTTAGAAAACAACATAGATA10.5847953216374269No Hit
GTATGGCAACATCAGCGCCGCCAGCGTGGGCGCCATCCAGCGCGGGTTGC10.5847953216374269No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT10.5847953216374269No Hit
GATAAAACCAACTCAACTCCAAAAGCTAAGAGAATGATTCTTCTCTCAGT10.5847953216374269No Hit
ATAAACATGATCCAAGCAATACGAGCACCTACAAGTGCTACACTAGACAC10.5847953216374269No Hit
ATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGCACCTTGGGATGGG10.5847953216374269No Hit
TCCCAAAAATGATCCAACAAAAATATGCAAAACAGAATTTACATATAATC10.5847953216374269No Hit
GCCCATGGAAGATCGGAAGAGCACACGTCTCCCCTGGAAGATCGGAAGAG10.5847953216374269Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTCATTATGGTGCAAATCCTAAAGAAGTTTGCTGCTACAGAAACGCATA10.5847953216374269No Hit
CTTGGGTCACATCAATTCCATCAACATATCGCTCGAGCAGAACAGCCGAT10.5847953216374269No Hit
GAATAAGAAGCAAAAACCCCTTTACGAAGCAATTCAAAATCCAAAAGAAG10.5847953216374269No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers