FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAAATCCTATGATGATATCCCATGCTAATGTATCCAGAGCGTAGGAT11.2048192771084338No Hit
GCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAGCGTATCAAAACC11.2048192771084338No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGC11.2048192771084338No Hit
TCTAAATCCATTAACGAGGATCCATTGGAGGGAAAGTCTGGTGCCAGCAG11.2048192771084338No Hit
GTCTAGGGCGGGTTAAAGAATGCCCTCTTTCTAGCCTTTTCGTTTTTTAG11.2048192771084338No Hit
GGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAA11.2048192771084338No Hit
CACAAAGACTCACATATCAAAGACCTATACAACAATACCACCCGCTACTT11.2048192771084338No Hit
TCTTCATTGCGGTGGTGAGAAACAATGGTCAAGTTTCAGAAGCAGAACAA11.2048192771084338No Hit
CGCTAACCTAGAACCCACAAAGGGTGTTGGACGATTAAGACAGCAGGACG11.2048192771084338No Hit
AATCAGCTCGCGTTGAATACGTCCCTGCCATTTCTACACACCGCCCGACG11.2048192771084338No Hit
ACAATGATCCATCCGCAGGTTCACCTACGGAAACCTTGATACGACTACTC11.2048192771084338No Hit
AAAGACATACATGTAATAAAAACCCAAATTCTCTCAGACAAGAGTATTCC11.2048192771084338No Hit
CTACTACCACCAAGATCTGCACCCACGGCCGCTCCGCCCGGGCTCGCGCC11.2048192771084338No Hit
CAACAGCCCTACACTTGGAACCTAACCCCTGATCGGCCTACAAAGTTGAC11.2048192771084338No Hit
GTGGACCCACTCAACTGCATCATACCCTAGAACATAAACGTTATGTAGCT11.2048192771084338No Hit
CTTCCGATCTCCAAACGGTTCTACCCGCCCCTCTGTGGTAATTATATATC11.2048192771084338No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTATGGTGTGCAATGGT11.2048192771084338No Hit
TACAAAGGCAAACCTTGTTAGTTTCTTTTCCTCAGATTATTTATATGATT11.2048192771084338No Hit
ATCGTGAACTATGCCTGAGCGGGCTAAAGCCAGAGGAAACTCTCGTGGAA11.2048192771084338No Hit
ACTACTCGGAAAACCGTAGAAATTCTAGACCTAATACGTCCAACAAACCC11.2048192771084338No Hit
CACCATGGTAGGCCACTATCCTACAATCCAAACATGATAGGGAACAAAAT11.2048192771084338No Hit
AGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGA11.2048192771084338No Hit
AGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGTTTTCCAT11.2048192771084338No Hit
ACATTTGCCTGACTTTTAACGTCCCAAAACTAAACAACAGATTACAGAAC11.2048192771084338No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC11.2048192771084338No Hit
GTAGTGGCCTACCATGGTGGTAACGGGTGACTGAGAATTAGGGTTCGATT11.2048192771084338No Hit
TGAATAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTC11.2048192771084338No Hit
TGTCGCTTCGGTCGGCGCTACGCTCCTGGTCTTAATTGGCCGGGTCGTGG11.2048192771084338No Hit
CATTATTCATCCGGACATATGATGAAGATGCTGAGGACTTTGACGAGAAC11.2048192771084338No Hit
CTTGATAGTTCTGTTTTGGTTATTTGCTCCTACGACCAAGCTCTTCACCG11.2048192771084338No Hit
ATTCTATGGGTGTAGGTGCATGGCCGTTCATAGTTGGTGCAGAGATATGT11.2048192771084338No Hit
CTACTAATCCACAAGCTCAACATCCACATCAACACTCTCATTCTTCTCCA11.2048192771084338No Hit
CGTGTGCTCTTCCGATCTTGCCCGGGAGACGTCTGATCTTCCCATCATGG11.2048192771084338No Hit
TCTAGTAGCTGGTTACCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG11.2048192771084338No Hit
GGAAGAATTCATTAATCAAGAACGAAACTTGGGGGCTCGAAGACGAACAC11.2048192771084338No Hit
CTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTA11.2048192771084338No Hit
GTCAAAAAAGCAACATCCCCAGAACCCTGGTCTTGATCGTAATAGGAAAA11.2048192771084338No Hit
GAAACAGCCTTCCGACCATACTCCCCCCCCACCCCAAAAACAAAGATTAC11.2048192771084338No Hit
CTGGTCTTAATTGGCCGGGTCGTGGCCCCGGCGGGGTTTAAATTTATAAA11.2048192771084338No Hit
ATACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCAATGAACTA11.2048192771084338No Hit
CGACCATACTCCCCCCGGAACACAAAAACTTTGATTTCTCATAAGCTGCA11.2048192771084338No Hit
GCTAAGCCTCCCAAACGCACCTGACGTACGCTAACTCCGATCGGAGCAAG11.2048192771084338No Hit
TAACTCACCCGTTGACTCGCACACAAGTCAGACTCCTTGGTCCGTGTTTC11.2048192771084338No Hit
AAGTAAAATAACGTTAAAAGTAGTCTAATTACCCATGCGCCGATTCTACC11.2048192771084338No Hit
CACAGAACAGCTACCGACAAGGGGAATCCGACTGTTTAATTACTGTCTCT11.2048192771084338No Hit
AAACTAGAGCTAATACCAGCAACAAACCCCCACAAATGCAAGCCACGCAA11.2048192771084338No Hit
AGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGG11.2048192771084338No Hit
CCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAAT11.2048192771084338No Hit
CTAAAACTGATATAATGAGCCATTCGCAGTTTCACAGTCAGAATTCGTTC11.2048192771084338No Hit
CCATTGGCTGTCCATTCGCTGGAAAGAAGACACGGACATTCCCAATCTCA11.2048192771084338No Hit
GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGACCCCAACTTTCGTTC11.2048192771084338No Hit
GATAAATACATTTAAATATTGCATGATGTCATGCACCAAAAAGCAACAAT11.2048192771084338No Hit
TTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTA11.2048192771084338No Hit
CCAAAAACCACGGACACACAAAGACACACAAAAAAACAGAAAAAAGAAAA11.2048192771084338No Hit
GAAGAGCAAACCTCACCAGGTGGATATAGGAAGACAACAAGTAACACAGT11.2048192771084338No Hit
CAGTGAACCAAATCGGACCAGCTCCACGTCTGCCACACACTAACACAAAC11.2048192771084338No Hit
AATACGAACCGTGAAAGCGTGGCCTATCGATCCTTTAGACCTTCGGAATT11.2048192771084338No Hit
ACCAAATATCAAAAAAATGCGACCCTTCCATAACTAGCGGATTCATGCAC11.2048192771084338No Hit
CAAAAGAGCAAAGCTCCGCCTGTGGCAAAGCCAACAGACAAGCCTCTGCT11.2048192771084338No Hit
ATATAAAGATTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCACTAA11.2048192771084338No Hit
GGTAAGGACATTGAGAAGGACCCCAAGCTATACAACAAATGTGACGCTCG11.2048192771084338No Hit
GAATCTAAATCCCTTAACGAGGAACAAATGGACGGCGAGACTGGTGCCAG11.2048192771084338No Hit
GGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTGCAAGACATAGGGCGCG11.2048192771084338No Hit
ATCATAATACGACCTTGCAGCTTGTAGATCTTCGTCATAAAATTTTAGAA11.2048192771084338No Hit
TCCTTAACCACCACCGACCCTGATCCAATCTTAGCTCCCTCACAGATTCT11.2048192771084338No Hit
TCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCCAAAGGCGCAAGGA11.2048192771084338No Hit
CATCAAAGAAACAGCGCCGGAGGCACGACCCGGCCAATTAAGACCAGGAG11.2048192771084338No Hit
AACCCGCACCGAAAGCTCTAGTGACCTTCAACGAACCTTTAACCCGTTCA11.2048192771084338No Hit
TCAAACTACAGTGCGATTTTTAAACCTTCCGAGTTTTTTTGAAGTAATTC11.2048192771084338No Hit
AAGAAAGCCAACGCTCTGGATACATTAGCATGGGAAAACATCATAGGAAT11.2048192771084338No Hit
CTAAAACACTAGTCAACCATAAGTTACAGTTTCAGAGATTGGATTGAAGA11.2048192771084338No Hit
CTATCCTACCATCGAAACTTGATAGGGCACAAATTTGAATCATGCGTCGC11.2048192771084338No Hit
GAAAAATAAATTTTAACATATTAGAAAATCATAGCTAAAAATTAAAAACG11.2048192771084338No Hit
ACTCAGAGAAGAAAACCCACAAAATCTCAAATACAAAGAGACACACGACG11.2048192771084338No Hit
GCCAAACTCCCCACCTGACAATGTCCTCCGCCCGGATCGACCCGCCGATG11.2048192771084338No Hit
ACTTAGCAGAGTCCTGTGTTTTTTATAAAAAAGTAGCTAACCCCTGGTAT11.2048192771084338No Hit
GCCCTATCAACTTTCCATGGTAGGATACTCGCCTACGATGGTGGAAACGG11.2048192771084338No Hit
TAAATGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGCG11.2048192771084338No Hit
GACCCTAAGCAATCACTTAGTGGAAAAGGAAGTGATCGAGCGATGACAAC11.2048192771084338No Hit
CTCTTTCGAGTCTGGTAATTGGAATGAGTAAAAACTAAATCCCTTAACAG11.2048192771084338No Hit
TCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAA11.2048192771084338No Hit
AGTTGTTGCAGTTAAAAAGCTAGTAGTACAACCTTGGGATGGGTCGTCCG11.2048192771084338No Hit
AATCCACCACCAAATACCGCAAATGTAGGAACAAGACAACAACACGTATA11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGTG50.0145.05
TGACATG50.0145.03
GATCGTG50.0145.0145
CTGACAT50.0145.02
ATGTGTG50.0145.07
GACATGT50.0145.04
TCTGACA50.0145.01
GTGTGCG50.0145.09
TGTGTGC50.0145.08
CATGTGT50.0145.06
Produced by FastQC (version 0.11.5)