FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6D2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6D2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAATAACAGCAACAACATAAATATACGCAATAGGAGCAGGAAATACCG10.9433962264150944No Hit
ATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC10.9433962264150944No Hit
GCGCATCAAAGGCATCATTCAGGTTTGCAAAGCTCTCTAAACACATATTT10.9433962264150944No Hit
GGATCAAAAATCCACGTCGCAATCAACGCTTCGCTGCCACAAGCCAGTTA10.9433962264150944No Hit
AACACACACTGTGGGAATGCCTGTCCTGAGGTTGCTGCCCTAAGCTGACT10.9433962264150944No Hit
ACGATATAGTCATCAGAGCCATCCCATTCAAGTCCAGAAGCTAAACCTCA10.9433962264150944No Hit
TTCTAAGGTTACATCCCGTGTCCCAGCCTCCACAGTCACATGATCCATAT10.9433962264150944No Hit
AGAAAAACAATCGCCTCAGGTGAGACCAAATGCCTATTGTCAGCGTTGAT10.9433962264150944No Hit
CCTCGAAGTCAGAATCCGGTCTAGAAGAGACGCATGCGCCCGACGCCCGA10.9433962264150944No Hit
AACCACACCATTGAGAGAACGCAAGATCAAAATTATAAATTGAGATATAT10.9433962264150944No Hit
AAACAGAAATTGGAAGAATTATTTCTGTTTTACCCAAGAAAACTACCTAG10.9433962264150944No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTCAATTCTGCTTCACAAT10.9433962264150944No Hit
ATGACAATACTCCCCAGTGCTCAGAAAGTCAAAGAGAACAAAAACAACCA10.9433962264150944No Hit
GCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAAT10.9433962264150944No Hit
TATATATACACAAAGAGAAAGGGTTTTGATTGGATCGGCTTTGGAGTCTC10.9433962264150944No Hit
CCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCAAATTACAAG10.9433962264150944No Hit
CTAATCAAAGCATACCTTATAACAAATAAAACGCAGATTTTACCGGTACA10.9433962264150944No Hit
GCTCAAAACAACCAACTTATCCGATACGCCCACGAGGGTTACAGAAAAGA10.9433962264150944No Hit
CAATAGCAATATCACCGAAATCCGCAACAATCAAAACCGTTTCACGCATA10.9433962264150944No Hit
GACCCAATCACATCATTCATAAAACCAAAAACCAATAAACCTTCATTTAA10.9433962264150944No Hit
GTGATTCGAAGCCCCGACATCGAAGGATCAAAAAGCTACGTCGGTATGTG10.9433962264150944No Hit
AATTAAACATTAATCCCAACCCTATTTAAAACAAAATACGATAGAAAAGC10.9433962264150944No Hit
CGTCTAGATCGCAAGACCACACGTCTACAGCTGAAGACCACACTTCAACA10.9433962264150944No Hit
ATTCATGCCAATGTCTATCAGACTATAAAATTATTGCATGATGGAACAAA10.9433962264150944No Hit
CTTTAAGAGCCCATCAAGTACAATTATCGATTAGAACGGTTACGTTTATA10.9433962264150944No Hit
GTCCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGG10.9433962264150944No Hit
CAGAATCGACGCAAGGCAGAAACAAAAAGGAATTGACGCAACCGCACCAC10.9433962264150944No Hit
AAGTTGCACCGAAGCTTCCCACGAGCTTTTCATAAGAACGGTCATTGACG10.9433962264150944No Hit
ATACAGATGTAACGTGGAATGTAGAAAGTGTTTTATATAACAGAATAACT10.9433962264150944No Hit
CCCACAAGACTTGCTCTTATCATGGCTATGATCACAGCTGGGCTAGATCT10.9433962264150944No Hit
GGTATGGATGGAGGTATCCCGAGAAAAGAAGGTATGCAGAGGAAGGATGT10.9433962264150944No Hit
CCCCGAAAGAGAAGTGTTGTTAGCACAGTGTGTACTCACAATCGAGTAAG10.9433962264150944No Hit
CTATGAATCGAACACACAATAAACAGACTTACTAACAATAAAGAGTAGAG10.9433962264150944No Hit
GGTTTGGATGGCGCCCATGACGTCTTGAGGACGCATGGAAATGCTGTTGT10.9433962264150944No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGA10.9433962264150944No Hit
TCACAAGAAAGATCGGAAGAGCACACGTCAAAATGTAGTGATCTTAATAG10.9433962264150944No Hit
GACGGAGACTCTTGATCAATTCTCACCGGAACTTTCTCATCCTAAAGAAA10.9433962264150944No Hit
CAACAAAGTTGCACTAGCAGAGCAAACCTCCATTCACACTCTCTCGTCTT10.9433962264150944No Hit
CATGTTGATCTTAAATCAACAAACCAATTCAAACATCAATTCAAAGCTTT10.9433962264150944No Hit
ATAAAATGTAATGGATCAAGTTCACCACCGCATGTCGCTACGCTCCAGGC10.9433962264150944No Hit
GCAAAAAAGATGCCACACCCTCCGAAACTCCGCTGCTAGCGATTCCAATA10.9433962264150944No Hit
CGAAGTGCCGCTCTCGACACATTAGTCCACCCATTCTTTGATGAGTTAAG10.9433962264150944No Hit
CTACTACTCGGATAACCGTAGTAATACTAGAGCTAATACGTGCAACAAAC10.9433962264150944No Hit
GAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTGTCGGGAGC10.9433962264150944No Hit
CTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTAT10.9433962264150944No Hit
GCATGGGATAACATCATAGGATTTCGATACTATTGTGTTGGCCTTCGGGA10.9433962264150944No Hit
CTACAGAAACACTTCGAGTCACCGTCGACAAATTTTCCGCATCCGACAAT10.9433962264150944No Hit
GGGAGACGTGTGCTCTTCCGATCTGAGCTGGGAGACGTGTGCTGTTCCCA10.9433962264150944Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CATAAAGACATTCGCAATTATAAGCTAGATCTCAAAGAAAATAAACCACA10.9433962264150944No Hit
ACGCAGAACCAGTTCATGCATATTAGAAAGGATGGGAGAGCCTTTCTCTG10.9433962264150944No Hit
ATACTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCATAC10.9433962264150944No Hit
ATCATATCATCATTCAGACATGCAACGAATAAATACATATACGTGCTAAA10.9433962264150944No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC10.9433962264150944No Hit
TACATACACAAGCTCCAACAAGCCTAAACATCTTTTTTTGGCATTGTCAT10.9433962264150944No Hit
CTCAGAAACGATCTATCAAAAGCAGAAGAAAAAACAAAAGATGCGAAAAA10.9433962264150944No Hit
CCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGTTACCACA10.9433962264150944No Hit
GAGCACGCTGATGCCGAGGCACGCCGTTAGGCGCGTGCTGCAGACCACGA10.9433962264150944No Hit
GCTAAAAACATTCGAGTAATCGTAAGTGATTTTTCTAGATGACGAAGAGG10.9433962264150944No Hit
GAACGAGACCTCAGCCAGCTAACTAGCTACGTGGAGGCATCCCTTCACGG10.9433962264150944No Hit
CGTCAGTACACTGTTCCTCTGCCGCGTTACATGGCCCTGATGGATCAGCA10.9433962264150944No Hit
CTCCGATCCCGAAGGCCAACAAAAAAGGATCGAAATCGTATGAATTAATC10.9433962264150944No Hit
GCTAGCGGATTTTGGATCGGCGGTTTAGTTTGAGAAATCTACGATTCATG10.9433962264150944No Hit
CTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTAGTTGTTCGCTATC10.9433962264150944No Hit
CCTCAGAGGCCTCTGTGTCTTTGGCAGGAGGACCTTCATGGACTGTGGTA10.9433962264150944No Hit
GTACAGAGCAACACTCCCATCTACATCCCCGGCAAGCAAGACATTTTGTG10.9433962264150944No Hit
TCTCCCACGTAAGATCGGAAGAGCACACGTCTCACACCTAAAATAGCAAG10.9433962264150944Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATGTAGCCAAGCGAACTCGGCAAAAAGGATCCGTAACTTCGGGAAAAGGA10.9433962264150944No Hit
GAGTAGGAAGAGGAGGAAGCAGAGGAGGTGAAGACGAAGAAGAAGGCAGG10.9433962264150944No Hit
GAGTTATATAACTCCTGCTACAGAAGCTACGTTTTAGTTTTATTGAAAAG10.9433962264150944No Hit
GCAAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTACCCCCGAAAAT10.9433962264150944No Hit
GCAGAATACTCCGCGAAACAACTAGGCTTTTCCGACATTCAGCTACACCA10.9433962264150944No Hit
CATGGATCTCTGTTCTAAATGTTACAGAGGCATTTGTGCTGAGGAAGCTC10.9433962264150944No Hit
GATAGCGCAAAAGCGCGAACTTCGAAAGGGGATCCGGTTAAAATTCCGGA10.9433962264150944No Hit
CAAACTATGAGATGCACAATCAACAAGCTGAAGATAACGTAGAAGACAAC10.9433962264150944No Hit
CCAACAACGTGTTTGATGAGTCACCGTTCTTGCTTATAGAAGCATCTGCA10.9433962264150944No Hit
GGTGCATGCAGCTCTCACATGTTTGAACTGTGATCCACTATCAGAGGCAT10.9433962264150944No Hit
CCAAACACACAGCTCCTTTAGCCACTGCAGATTCAAGATACGTGATGGGC10.9433962264150944No Hit
AAACGGCCATAGTCCCTCTAAGAAGCCGGCCGTGAAAGGGATGCCTCCCC10.9433962264150944No Hit
AAATTATCGTTGACACTAGCACGGTATCTTAACTTATTAGATCCAAAAAC10.9433962264150944No Hit
CTTCTAAAACATTTGAAATTACTAGTCGCTACTGATTCTAATTCTTTAAA10.9433962264150944No Hit
TTTAAGTTGTTGCAGATAAAAAGCTCGTAGTTGAACCTTGGGATGGGACG10.9433962264150944No Hit
CTTCATAAGAGCCTCCAGGCATCATAGATGGGCCATTACGAGCACCTTCT10.9433962264150944No Hit
ACCATCCCGATTCTACAACAATGTGATATCAGCCCAAAGGTCTGAATGTC10.9433962264150944No Hit
ACACAGAGAGAGAGACATATTTTTGAAAAAGTAATATCAGACAATCCCGT10.9433962264150944No Hit
GTTTCAGCCTTGCGACCATACTCACACCGGAACACAAAAAATTTGAATTC10.9433962264150944No Hit
GTGAAGACAAAGCGAGTTACTATCATTGCGATGTCACGAACGAGACGGAA10.9433962264150944No Hit
GTTGGTCTTACAAACTATGCTGCAGCTTACTGTACTGGCCTTCTTTTGGC10.9433962264150944No Hit
CCTAAGTTATAATTAAACAGTCGGAATCCCCAACTCCCTACCAGTTCTGA10.9433962264150944No Hit
CTTCTAATCAGATCCACCACCTTGTCTGGGTGAAGATAGACTTTATCACG10.9433962264150944No Hit
ATATGAAGCCATTTATCGATGCAAGTGCAATGAAAGTGATGGTTACAAGG10.9433962264150944No Hit
GCTGCATACTTTGTGGGATGCAACATTATGAAAGCTGCACCAAAGCAAGC10.9433962264150944No Hit
GAAAACATCCTTGGCAAATGCTTTCGTAGTTGTTCGTCTTTCATAAATCC10.9433962264150944No Hit
GAAGGCCAACGCGACCTCAACATCTTTTCCCAGAGAAGAAGCAAAGACGC10.9433962264150944No Hit
ACCCGGAAGATGCTCAACAAACCGTATTCTGCTTCGTAGTCGAAGAAAAC10.9433962264150944No Hit
GTCCGAGGTTGCAAGAGGCGGCGATGAAGCTATACTCGAGGAAAACAGAG10.9433962264150944No Hit
ACATAAGGAAAACATACATAGAAGCCATACATTTTTGAACTTTCATATAT10.9433962264150944No Hit
TTCAGAAGGAGTCAAAACTCCACTTGGTCTTGCCACAGCGAGTTGGTATT10.9433962264150944No Hit
GGGACAAGCCGACCAATGCACACCAAAGGCGGACCGGCCGACCCATCCCA10.9433962264150944No Hit
AAGGCGGACCGGCCGACCCAACCCAAGGTTCACCTAAGAGCTTTTTAACC10.9433962264150944No Hit
GTTTCTGACTTTTTTCCTTGGCTAGCGAGGTTCGATCTTCAAGGACTTGT10.9433962264150944No Hit
GGATCTGAAACTCCAGTGGCTTCAGTGATGAGAAAACCTCCTGGAGTCGT10.9433962264150944No Hit
GTTGTAACAAGATGGATGCCACTACCCCCAAGTACTCCAAGCCCAGGTAC10.9433962264150944No Hit
CTAAGAAACCTAAGTTATAAGAAGAGGTGACTTGACTACATTTTATGGTT10.9433962264150944No Hit
TTACAGACCTTCATCAAGTGTTTGATATCATAAACCCGCGGGAAATACAC10.9433962264150944No Hit
ACAAAAGAGAGACACAAAAAAAAAACTATATCCATGGATTCTAGAGTATC10.9433962264150944No Hit
ATCAAGTGTTGCGCTTGGAAATGGAAGAATCTCTTTATGCTTCTTTTCGC10.9433962264150944No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers