FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6D11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6D11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGGTAACCCGACACGCAGAATAAGCCAACCATACCGGAGAGGCAC11.7857142857142856No Hit
TCTCACACCAAACCCAATTCCCAATGAAAGTACCAAAAAAAAAGGATTTA11.7857142857142856No Hit
GACAGGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCGAGTC11.7857142857142856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.7857142857142856No Hit
GTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAAA11.7857142857142856No Hit
GTTATAGTTTGTTTGATGGTAACTACTACTAGCATAACCGTAGTAATTCT11.7857142857142856No Hit
GTTCAGCTCTATACAGAAACATAAACTAAAATAATCAACAGCAGAAGCTT11.7857142857142856No Hit
AAGTTACAGGTTACAACAAAGGAGAGAGCTACAAAACCCAAAATTATGGG11.7857142857142856No Hit
GTAAAAAGACCAGAGACTTCAATTTTCACAAATAAAAGGGAACTATCACT11.7857142857142856No Hit
TCTCATTACCCTCACCACTGAGAATAGCTGCCTGGACAGCAGCACCATAT11.7857142857142856No Hit
AACCAGGGCTCATCATAAAAAACATTCTCAGAGCAAATTACAAGTTTAAT11.7857142857142856No Hit
CAACAGCACCTGATGCAGATGCAGCCCATGATGGCTGGTTACTACCCCAG11.7857142857142856No Hit
ATCTTTGTCTGCTTTGTATTGTACAATGTAAATGCAATCCCTACCACGGT11.7857142857142856No Hit
ACTCAAAAACACAAAAACACCGATACAGAAACAACACACCTTGACCGAAA11.7857142857142856No Hit
TTCATGGAGAAAGTTGCGAAAACTGTTGATGTTGAGGAATTTTCAGTTGA11.7857142857142856No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT11.7857142857142856No Hit
GTTTCAGACTTGCGACCATACTCCCCCCGGCACCCAAAAACTTTGATTTC11.7857142857142856No Hit
CACATATCCGAAGATGAGAGAGGTGATAATGAGAGGGATCACAAGGGCAG11.7857142857142856No Hit
TTGATCGAGAGAGTCAGAGGAGCGTCGCGAGAGTTCTTCGAGCTACCGCT11.7857142857142856No Hit
TTCATACACAAAATGTAAGACCTATCTTTTACTGAAACAATAACACAAAA11.7857142857142856No Hit
TTCTTTAGTCTGTGTTGAATGTACATCTTAGAGTAGTTGCATGTACTCGA11.7857142857142856No Hit
AAGCTTCTCTCTGTATTGTGCCAATCTCTTTGTATAAGCATAGACACCAG11.7857142857142856No Hit
CATAGCTGGTAACAGCCCACATAAAGCAGCACTGCAACTACAGCAACAAC11.7857142857142856No Hit
CGCGAAGAGCAAGATGGATGATGAATTGTTGGAATTGCAGCGTCAGTTCG11.7857142857142856No Hit
AATGGGAAGAGCGGCTTAAGCCATTAGGTGTAGGCGCTTTCCAAAGGTGG11.7857142857142856No Hit
AAGTTACAGAAAACGGCTAATGCACAGGAGAGAAGAATGCAGAAGATGGC11.7857142857142856No Hit
CGTCCTACCTATTTAATGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCA11.7857142857142856No Hit
ATAAAAATCCCTTAACGAGTATCAATTGGAGGGCAAGTCTGGTCCCACAA11.7857142857142856No Hit
GCTTGGTATCGAAGAAATCCCCGGCATTGTCAATGATTTATAGGCTTGAT11.7857142857142856No Hit
TCATCTATACAATAAAAAACAAACAAAGTTAATGCAAAGAACAATATCAA11.7857142857142856No Hit
CATGTGATAAAGCCTCTTATCCCTGCTAACTACCTTGACGATAACACAAT11.7857142857142856No Hit
CTTACTATCTTACCTCGCAGAAGTGAGCACTTACCATCATACCTGTTAAT11.7857142857142856No Hit
GTATATTTAAGTTCTTGCAGATAAAAAGCTCGTAGTTCAACCTACGGATG11.7857142857142856No Hit
GGAAAACCCGTCTGATAGCCCTTAAGCGCGAACTTCGAAAGGGCATCCGG11.7857142857142856No Hit
ATTGTGATGTTTATTACACTCTTGTCTAAGAAAGGAGATTAGAGTGATTG11.7857142857142856No Hit
TACCCAAACAGCCCGACACCAACACCGCGCCACGACCAGCGCCACCCACC11.7857142857142856No Hit
CTTAACACTAGATCGTCTCTCGAGGTTAAATTTTATTACTTGGTAAGATT11.7857142857142856No Hit
TACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCC11.7857142857142856No Hit
ACCTCGACGACCACAGCCGCAACTGACCACCCCCCGGCCTACACTACCGC11.7857142857142856No Hit
GTATATGAGTATGTGGAAGAAATCATAATATCTTGGAAGCAGACATGAGA11.7857142857142856No Hit
ATTCCATGAAGGTTCAATCTCACTTTCACTTTAAGCTTTGCCCTCTCGCC11.7857142857142856No Hit
AGGCTGGGTGGTCAGCAGTGGTTTTGTTTTGATTAAGTTTTAGACAAAGG11.7857142857142856No Hit
GAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAAATACCCA11.7857142857142856No Hit
CCATAAGAGACTCATAGTTACTCCCCCCGTAAACCCGCCCTAGGAACAAT11.7857142857142856No Hit
GTGTAGAAAGGTCGACATCAAGATCATTCACTTCTTCGTTCCCTGTACGA11.7857142857142856No Hit
CAAGGAAGGCAGCAGCCGCGCAAATTACCCAAACATGACACTGGGAACTA11.7857142857142856No Hit
TACCAGACTCGAAAGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCC11.7857142857142856No Hit
TAAAAAGTCAAAGCATCCGAAACTATTAAACTCTTATTGAATAGTGTGCA11.7857142857142856No Hit
CGGTAGTATGAAGTGCTACTCGAAATCTTTGCTACCCGAATCTTTCCAGT11.7857142857142856No Hit
CAACAGATACCATCCTAGTAAAAAAAAAAAACGAAACACCCCAGCAATCA11.7857142857142856No Hit
CCTTAAACGATCGGTCAAAACTATCGGAGAACATCGTAAAAGCCTGTGAG11.7857142857142856No Hit
CCTGTAAACACTCTCTCTAACTTGGCAATAGTCTCACAAAGTAACGTACA11.7857142857142856No Hit
GTAATCTTATTGGCACTAGCAGTGATTGTGCCATCTCTATGATCGAGTTC11.7857142857142856No Hit
ATGACACTACCTCCCCGTCACAGGAATGGCTAATATCCGCGCCTCCACCC11.7857142857142856No Hit
TAGAACAAACAAGAAACGACTTCAACGACTTGACAGGTATTTCAGATCCT11.7857142857142856No Hit
TCGATATGGAGTCTGAAAATCGTAATAGATCCGAGAAGAAACCGAGATAT11.7857142857142856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA50.0145.05
TATACAA50.0145.06
ATCTATA50.0145.03
CATCTAT50.0145.02
ACCAAAC50.0145.0145
TCTATAC50.0145.04
TACAATA50.0145.08
TCATCTA50.0145.01
ATACAAT50.0145.07
ACAATAA100.072.59
Produced by FastQC (version 0.11.5)