FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23.7037037037037033No Hit
GTATGATAGGACTCTGCTTGTGGCGGATCCGAGGAGGTGTGAGCCGAAGA11.8518518518518516No Hit
TATCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGG11.8518518518518516No Hit
GTTATAGTTACGGCCGCCGTTTACTGGGGCATCCATCAAGAGCATCGCCT11.8518518518518516No Hit
GTGGAGAGAAGAAACCACCTCCGGTTTCTAATAAACCCTCTACCATACTC11.8518518518518516No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA11.8518518518518516No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG11.8518518518518516No Hit
GAGATACAGTGATAATCAGGCTAAAAAAAAAGGTCTATAAACAGGACACC11.8518518518518516No Hit
GCTATATACATTCCACAATGACGATTTTGTCAGACTGTGAAGAAGCCTTA11.8518518518518516No Hit
CTCCGTCGCTGACCACGCTACTCCACACAACAAATCCAACGCCGAAGACG11.8518518518518516No Hit
TCCCCGGGTTGCCCAAAAACAATGAGTTCTAATAAGGTGCCAGCGGAGTC11.8518518518518516No Hit
TGTTCAGACTCGTATGATCAAGCCTGGTATGGTTGTGACCTTTGCTCCCA11.8518518518518516No Hit
GCACCAGACTAGCCCTCCAATGGATCCTCGTTAAGGGATTTAGATTGTAC11.8518518518518516No Hit
ATCTTATCGCACGAGGTAAGAGTAAGAAGACCAAAAGGACTGCTCCTAAG11.8518518518518516No Hit
ATATTCAACCAATCGTGTGTGACAATGGTACCGGTTTGGTGAAGGCTGGA11.8518518518518516No Hit
GTTTCAGCCTAGCGACCATACTCCCCACGGAACCCAAAAACATTCATTTA11.8518518518518516No Hit
GGACATTAAGAAAGCCCTTGTGAGTTGCGATGGTCTCAGAGAATCCGTAG11.8518518518518516No Hit
GGATAAGGAAGTTGATGCAGAGTTTATGTACACTGTGAAGTGGAAGGAAA11.8518518518518516No Hit
GGATTAACGAGATTCCCACTGTCCCAGTCTACTATCCAGCGAAACCACAG11.8518518518518516No Hit
GTCCTTACGAGAAGGAGCGTCTTGATTCTGAATTGAAGCTGGTTTGTGAG11.8518518518518516No Hit
GAACAAGACCAACCAAACCGATCTTGTCCTTAGCAGCAGAGCCTTTAGGG11.8518518518518516No Hit
ATCATCGGATCTCACCCACTCGAATCGTCAAACGAATATGAAAATAAATC11.8518518518518516No Hit
CTTCGGCCTACAAAGTTCTCATTAGAATATTTGCTACTACCACCAAGATC11.8518518518518516No Hit
GAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATCTAATAAA11.8518518518518516No Hit
TCTTCGAGCCCCCAACATTCGATCTTGAAAAAAGAAAACAACCTAGGCAA11.8518518518518516No Hit
GCTTTACCGGTTTCTTGTAAGGAGAACTAATAGCAAGTTAAATGGTGTGA11.8518518518518516No Hit
TGCAAATGTATCCATAGCGTAGGCATGCTTTGAGCACTCTAATTTCTTCA11.8518518518518516No Hit
TCCTTAATCTTCTTCTCAGCAGTCTACGAAATAAGCTTCGCCTTCACAAC11.8518518518518516No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT11.8518518518518516No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGA11.8518518518518516No Hit
ACAATAGAGAACATGAGAACATATAGAAAGAAACAGAGAGCATGACACAT11.8518518518518516No Hit
GGCTAATATCGTGGTTCCTGTTACCCGGAAGACTCGTCCAGCGAGTTCTC11.8518518518518516No Hit
GTAATCATTAACAGGGACAGTCGGGGGCAATCGAATTTCATAGTCAGAGG11.8518518518518516No Hit
CCTACGAGCCGAGTGACCGCGGTCTCCCGGAGGATCCCAGGACATTCGTG11.8518518518518516No Hit
TCATACTACCGTGGGTTTTTTAAACCTTCCGAGTTTTGTTGATGTTATTC11.8518518518518516No Hit
TAGATAGACTTAGATGATGCCGATCAAGTTAGCAACAAACAAAGCATCAT11.8518518518518516No Hit
AATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATATAAAAAAT11.8518518518518516No Hit
CAAGTACCGCGAGGTAAAGATGAAAAGGACAATGAAAAGAGAGTAAAAGA11.8518518518518516No Hit
AACCAGGCAAGAACAGCATACCCCATCTTTGCACGATGAAGAACAAACCC11.8518518518518516No Hit
CTATTGTGTTCGCCTACGGGAAAGCAGTAATGATAAACAGGCACAGTCGT11.8518518518518516No Hit
GACCGTCTGCCGCCACTCAGCAGAAAGCTCACAAGCATGCACTACTGCTT11.8518518518518516No Hit
GCTAAGGATGAGGGGAAGCAGACCTATGATTACGGTCGCAAAGGTGCGAT11.8518518518518516No Hit
TTGTTGCTCAACGGGTTCGGCTTCAATGTTTAGACCACCAGCCAAGTCAT11.8518518518518516No Hit
TACATTTGCAACTCCCTGTCTCCTCCCTCTAGCAATTGTAACTCCGAATT11.8518518518518516No Hit
CTTCCGATCTCCCTTGGGAGACGTGTGCTCTTCCGATCTCACTAGGGACA11.8518518518518516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCACCCACCAATAGGGAACGTGAGCTGGGTTAGACCGTCGTGAGACAG11.8518518518518516No Hit
AGATTAAGTCGGTGGAGGACATAACATTGATTGACCGCTTTGTGGAGCCG11.8518518518518516No Hit
GGTTTCGCATGGCTTCACGGCGCCGCTGCTGGCGGTGTCGGCTGTGCTGC11.8518518518518516No Hit
GAATAACAGCCTGCCCACCCTGGAAACGGCACAGCCGGACGTAGCGTCCA11.8518518518518516No Hit
TGAATACACTTACGACGATGATCGGTTAGCTGGGTGGGTTGTAACAACGC11.8518518518518516No Hit
GATTATGGATCAGACTAGGACGGTAACTGATCGTCATCCAGCCCCCAACT11.8518518518518516No Hit
AGCAAAAACGTCCAACAAACCCCGACAAATCGAACCCACGCATATAATAG11.8518518518518516No Hit
GTTAAAGTTAGATTGATGGAAACTACTACTCCGAAAACCGAACAAAATCT11.8518518518518516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAC50.0145.07
GTCGCTG50.0145.05
CCGTCGC50.0145.03
CTGACCA50.0145.09
TCGCTGA50.0145.06
GCTGACC50.0145.08
CGTCGCT50.0145.04
TCCGTCG50.0145.02
CCCCCCC50.0145.0145
CTCCGTC50.0145.01
Produced by FastQC (version 0.11.5)