FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6C8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6C8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC15.263157894736842No Hit
AGACAGTAGGGAAAATCGTTACTCCATTCGTGCAGGTCGGAACTTACCCG15.263157894736842No Hit
TCCCTTGATAGATCGGAAGAGCACACGTCTCCCTTGATAGATCGGAAGCT15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCCTGCTGAGATCGGAAGAGCACACGTCTCCCTGCTGAGATCGGAAGAG15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGGATATACTGCATAGTGATGAAGTCTGTGCTTTTAGTGCACCCATCACT15.263157894736842No Hit
GAGATCGGAAGAGCACACGTCTCCCTGGAGAGATCGGAAGAGCACACGTC15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTCCCATCCTTGTGTTACATATTTTATTTGTACATATTTTATTACCAGT15.263157894736842No Hit
CCCTTGTCAGATCGGAAGAGCACACGTCTCCCTGACGAGATCGGAAGCGC15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTATAGTCTTATTTCAACCTTACTATCCAAATTCATTCGTGTACTTTGT15.263157894736842No Hit
GCTCGGCACAGGGTGTCCAGTGGCAGATCATAGCTCTCCAACCCAAAAGG15.263157894736842No Hit
AAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGG15.263157894736842No Hit
GTGCTCTTCCGATCTCGCCAGGGAGACGTGGGCTCTTCCGCTCACGCCCG15.263157894736842No Hit
CGTCTCCCCTGTAAGATCGGAAGAGCACACGTCTCCCCTGTAAGATCGGA15.263157894736842Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TACAAGTACCGTGAGGGAAAGGTGAAAAGAACCCTATGACGGGCTGTCTC15.263157894736842No Hit
TTTAAGTTCTAGGGTACAAACCTGCACATCTGTCTCTTATACACATCTCA15.263157894736842No Hit
TTCCTATGGTTCGATCCTTCCCAGTAAAACGCGGCGTGTTCGAATTCTGA15.263157894736842No Hit
CTGCTTTAGAAGGTTCAGCAATCCTAATTTTTTTACGGGGTTTTAGGGAA15.263157894736842No Hit
AGAGGTGGCTGAGGAGCAGCCACTGGCGCAGGAGCTGCAGGAGCAGCAAC15.263157894736842No Hit
ACTTGGCGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGT15.263157894736842No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)