FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6C5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6C5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT18.333333333333332No Hit
CTCGTTGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACT18.333333333333332No Hit
GCTTGAAACAGGACAAGAAGCTCGCGTCCGAAGCGCACATCTTTTTGCTT18.333333333333332No Hit
CTTCCGATCTAGCCCGGGGGCCGGGTCGTGGGGGCGGCGCGGGGTAGTTT18.333333333333332No Hit
CGCCTCAATACACTATGTATTCATGTATTGATACCTACCTTATGGTAAGT18.333333333333332No Hit
GGGCAAGATTCTCTACCCGTTGGGTAGACAGGTACGGGTGTTGGTGGGTA18.333333333333332No Hit
ATGCTAAGGGCCCGAAACGTACCTAAAGCCACTTCGGTGGTTATGGTGCG18.333333333333332No Hit
GAAGAGCACACGTCTCCCAGTGTAGATCGGAAGAGCACACGTCTCCAAGT18.333333333333332Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCGATCTGCTACGGGAGACGTGTGCTCTTCCGCTCTGCTACGGGAGACGT18.333333333333332No Hit
CTCTTCCGATCTGCACTCGGGGGTCAAGATAGTAAGGGTCCACGGTGGAT18.333333333333332No Hit
GTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT18.333333333333332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCCAGGTGTTAAGAAGAGGAATTGAACTTCTGACTGTAAAGTTTTAAG18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)