FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6C2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6C2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATTCAATCACACCTTTCATTTTGTGTAACAATTCTAAATCAATTCC15.88235294117647No Hit
GATTATCACCCGCTCGGGGATAATGTCTTTGGCAATGAGCAATCTCGAGA15.88235294117647No Hit
ATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGT15.88235294117647No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA15.88235294117647No Hit
TCCCTGAGTAGATCGGAAGAGCACACGTCTCCCTGAGTAGATCGGAAGAG15.88235294117647Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCACTCAATCCAGTTTCATTTCCGCCAAAAGTCTCTTCCTTTCAGCAA15.88235294117647No Hit
GGTTGGAAAGCTGCAGCTGTTGGAGCAAACAATCAGGCGGCTCAGTCTAT15.88235294117647No Hit
TTCAAGCTGGGCTCTTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTG15.88235294117647No Hit
ATATTTTTCCATTTATTTTCTCGAATCTTCTTCTTCTTCCTAGATTCCAG15.88235294117647No Hit
CTTCACAGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGC15.88235294117647No Hit
AATTAAGACCTTGAGGTATCTCAGCCAAAAACTCCTGTGAAGCATCAGAG15.88235294117647No Hit
TTTTTATCCACATCAATCGCATCAAATTGATCTCTGAGGTCAGAGATCTC15.88235294117647No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG15.88235294117647No Hit
CTTCAGGCCAACGCGTGCAATTCAGTGATCTCTGGGATCAGAAAGATGGA15.88235294117647No Hit
ATATCATTCATGCTTTCTTTATTCACTTTGAGGGTCCGTGGTGATTTGTG15.88235294117647No Hit
CCCCAGGGAAACTCCGAATACAGGCCGTTCTCGTTTGTACAGACAGACTT15.88235294117647No Hit
CAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCA15.88235294117647No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)