FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6B8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6B8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29.523809523809524No Hit
CACCGGTACGGAACATACGTAAGAGTACCCTCCGATATCCGACATCATGA14.761904761904762No Hit
GTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCCGGCCTAGGTGATTCG14.761904761904762No Hit
CATCCGATCTCCACCGGGAGACGTTTGCTCTTCCGCTCTACAACTGGAGA14.761904761904762No Hit
CCACTTACACAAGGGAAACTCCATCCAAAGACACGGCGGAAACCCGACAG14.761904761904762No Hit
GGGTAGTAATGGGTGAATGGCAACTCCGACTCGATCATCATTGTGGCAAG14.761904761904762No Hit
GTCCTATTCGATGCCGAAGCCCTACCCGAACACCACCACCCACTCCAGCC14.761904761904762No Hit
GACTCACCCTGCTCCGATGAACGTTGAACAGGAAACCTTGGGCTTACGGC14.761904761904762No Hit
ACTCCCCTTAGAGATCGGAAGAGCACAAGTCTCCCCTAAGAGCTCGGATG14.761904761904762Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCACCTAACCAGCTGATGCGTGACGCTGCTCCAGCCACAGAACTCTCCGC14.761904761904762No Hit
ATTCAACGAGTTTGATGCAAGAACTCGTTGGACAATTAAGGTCTAAGAAC14.761904761904762No Hit
GGTCTCCATCAGGCCGAGCTGCGGCTGATCCAGTGTGAGCAGGAAGCCGT14.761904761904762No Hit
GAAGAGCACACGTCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14.761904761904762No Hit
CTTAATCTATACAACGACGACTGAGATAAAAGATACATACAAGAAAAAAG14.761904761904762No Hit
GGCCAGGCCTCGATGAGTAGGAGGGCGCGGCGGTCGCTCCAAAACCTAGG14.761904761904762No Hit
TTCCAGAGTCTCCACATTGGCCGTAATCCATCCAAGACCGAAGTTGTTCG14.761904761904762No Hit
CCACGGGGGTGCGTCTGCACGACTACGGCTGCGCGCTCAAGGCCTATCGG14.761904761904762No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT14.761904761904762No Hit
GATCGGAAGAGCACACGTCTCCCAGGCGATATCGGAAGAGCACACGTCTC14.761904761904762Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCTTAGCAGCTGCGTGCGATCGCGCACGTTCAGCGTCAGCACCAGCGAG14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)