FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6B5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6B5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATTGTACTCATTCCAATTACCAGACTCGAAAGAGCCCGGTATTGTTAT12.1739130434782608No Hit
GGCAAGGCGCGAGTTGGATCACAAAGCTGCTCACGCTTCCCATTATTATC12.1739130434782608No Hit
TATGACAAGAGCCAAATAGATAATTTGGGAGTGAAACTAGTTAGTTGGAA12.1739130434782608No Hit
CCAAACTCTATCGTCAACTTCAACTAAACAACCGCCCTTTCAACATCAAA12.1739130434782608No Hit
GGATCGAACCAATTGGAAGACGAATATGCTCACATCACTTCTCATTCCTT12.1739130434782608No Hit
GCCGAATAGAAGACTGTTGATGCGGCATAGGTGGAGCTGGCTGTGACATT12.1739130434782608No Hit
GACAAAGGGATCGAAAATTTCTACAGTCTCCATTCGTGGCTCGGCCGTGC12.1739130434782608No Hit
GGCGAAAGACTAATCGAACCAGCTAGTAGCTGGGTCCCTCCGAAGTTTCC12.1739130434782608No Hit
AACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGCGACCTAAACCCGGCC12.1739130434782608No Hit
TCGCCGAACGAAGGGACAAGCCGACCAATGCACACCAAAGGCGGACCCGC12.1739130434782608No Hit
AAAAACGAAAGCCCCCGACTGTCCCTGTAAATCATTACTCCGATCCCGAA12.1739130434782608No Hit
CTGTAAATCATTACTCCGAACCCGAAGCCCAACACAAAACCATCGAAATC12.1739130434782608No Hit
TACTACGAGATCATATAGGTTGACCAAGAACACAAAGCTCAGCCTAATGA12.1739130434782608No Hit
GAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAG12.1739130434782608No Hit
AAACGAAACTAATGGACTCTCCACACAATATATCCTGTAGTTTCTCATGC12.1739130434782608No Hit
GTTAAACACAAAAGATAACTCTTCACGAGGCCCCCGCCGACGTCTCCGGA12.1739130434782608No Hit
TCTGTTAAAAGATAACCCAGGAGTCCAAAGAACAGCTCAACGACAACACA12.1739130434782608No Hit
AAACACGAAAGAACTCGCGAACGACCGCCAGCAATAGTGATTGAAACTAC12.1739130434782608No Hit
GAATAAGCATTGTGGAAACATAATTTGTACATTCCACTTTTTGGAGCCTT12.1739130434782608No Hit
CTCTCTGGCGATGAATCTCGTAACTCCCTCTGGTTCAAATCCGGTTCTCC12.1739130434782608No Hit
GCCTCGGCATCAGCGTGCTCCGGGCGACGGCCAGTGGGCTCCCCATTCGA12.1739130434782608No Hit
CTTCCGATCTGACTGGGGATTCAAATGAGAACTTTGTAGGCCGAAGAGGC12.1739130434782608No Hit
GCACGAGAGGCAGTGTTAATCTAAAGAATCTAGTCGGCTTGGCCAAAGTA12.1739130434782608No Hit
CACCCGATAACCGCACTGCAAATAGAATCGTCCATATGGTCGAGAGACGT12.1739130434782608No Hit
GAGGCATTGGGGGCGCAACGCCCTCGACCAATTCTCAAAACTTTAAATAG12.1739130434782608No Hit
CTATTCTCTTGGCCTTCGCGATCGGACTAATGATTAACACGGACAGACCC12.1739130434782608No Hit
GTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCATCCGACGTCTCCGGA12.1739130434782608No Hit
GATAGAACACGCCTCCGACATACAGCTAACCAGAGAGAAACTACGGAGGG12.1739130434782608No Hit
TCCATATACTATTAATACTAATGTTTACAAAAAACGCACAGGAGACAAAG12.1739130434782608No Hit
TCTCACAACTCTAGCACCGTAACTAGATCCTCCTGCTCCGATACCACGAC12.1739130434782608No Hit
GATCGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGAA12.1739130434782608No Hit
GTAGGCTTCCTATCAGCACTCTAATATCTTCAAAGTAACAGCTCCGGAGG12.1739130434782608No Hit
GGTTGATCTTGTTCAAAAAGAAGTGACAATTTTGTTACAATCTGCTCTCG12.1739130434782608No Hit
GTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAAT12.1739130434782608No Hit
CTAGGGCGGGAAGTCAGTAACTAGGAAAATTCAGAAGCGCTCTCAATCTC12.1739130434782608No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTATTGACGCGCAAGAAT12.1739130434782608No Hit
GATGAAATCAGTATGAGCGACCAAAGTCCTCCACATTAAGCAATCTTCGT12.1739130434782608No Hit
CACACCAACCACACACTCACAGAAACTTTAAAACGATCGTATGATCACAA12.1739130434782608No Hit
GTTGTTTCACACTCCTTAGCGGATTTCGAATTCCATGACCACCCACCAGC12.1739130434782608No Hit
GACAAATACTTCTCCTTGTAATTTCCAAAAAGCATGGCGAGAGATATGAA12.1739130434782608No Hit
CCACTATCCTACCATCGAAAGTTGATAGGGCAGAAAATAGAATCAAGCCA12.1739130434782608No Hit
CATCTTGATAGATTAGCAGGACTAAGCAGAGGGAAAGCTTCCCAGAACCG12.1739130434782608No Hit
CTTCCGATCTCGCCTGGGTACCTGGATCATCCTGCGAGTAGTCATATGCT12.1739130434782608No Hit
CCACTAAAATGGACCTTTTTATGTATTTCTGTTAAGTAAAATCACCCAGT12.1739130434782608No Hit
TCTTCACGTAGTCTTCCTTTAGCCACCTTAGCTAATAACTCACCGCCTAG12.1739130434782608No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGA12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)