FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6B3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6B3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTGCTAATATCGAAAGAACTAAACCTACTCCGATACTATGTAGATGA11.2048192771084338No Hit
GTACATATTATTTCTTTGTACAGAAGAAAGCAAAACAAGGAAAATGGAGA11.2048192771084338No Hit
AACTAGCTGTCTCGCATGTAAGGTTGAGACCCAAGCTTTAGCTGCACCAG11.2048192771084338No Hit
AGATAAAGTCCAAGAACTTTGCCTTTCCCTTTATAAAATCTATGGAACTA11.2048192771084338No Hit
GTAGATATCGCCTCGAATTCGGCATGGATCTCCGCCGGAGCGACTCTCGG11.2048192771084338No Hit
GCCAAGAACATCTTCGTTACGGTTTGCTAATTATCGTAATAAAATCAACA11.2048192771084338No Hit
CCAATGGATCCTCGTTAAGGGATTAAGATTGTACTCATTCCAATTACCAG11.2048192771084338No Hit
CAGGGGAAAGGAACCATGTCAACCCCACTAGCACATGCGTTAGTCGATCC11.2048192771084338No Hit
CACTCAGCCTGAAGAATCACATCAAGACCCTTACACAACCGCACGAAACC11.2048192771084338No Hit
CTTCTCTGCAGACCGAGAGCGCCTAGGCGGTCTAAGACGTCTCATAAGAT11.2048192771084338No Hit
GTTCTGTATTCTGCAAGAGCATACTTGAGACTCTGCTCATCTTCCGGTAG11.2048192771084338No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCGTTTGTGTGCATTGGT11.2048192771084338No Hit
AAGAAACTCCCGCCGTTTACCCGCGCTTGGTTCAATTTCTTCACTTTGAC11.2048192771084338No Hit
GCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAG11.2048192771084338No Hit
ATCGTAAACAGAAAGAAGTCTGCAAAATACGGTACAGAAATAAAGATGAG11.2048192771084338No Hit
CAATACATGTAACCATTGCAATGGCTTCACCTAGTAGAACCATCGAAAAC11.2048192771084338No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG11.2048192771084338No Hit
ATATTCTTCAAATCCTTTCCATGAATGTGCTTCAGATTGTTTAGAGAAGA11.2048192771084338No Hit
ATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGG11.2048192771084338No Hit
GGATGGTCAGACCCGTGAGCACGCTATTCTTGCTTTCACCCTTGGTGTCA11.2048192771084338No Hit
ACCCTATGAAACCTATGGATACCCGAACTTAAAAAGTGTCAAGGAATTGA11.2048192771084338No Hit
TATTACAACAGACCTCGACGTCAAGCCCCCTCAGAGAAAGTCTCACACAC11.2048192771084338No Hit
GACAAAGGAAATCGAATTAAAATCTTATTCACATTCGAGAGACGACGACG11.2048192771084338No Hit
AATAATAATAGGTCAAACAAGATTGTACCTGACCAACTGACAAGTGATCA11.2048192771084338No Hit
CACCAACAACAAACATTGGACCGTCTATGCTGGGGGCAGAGATAACAACC11.2048192771084338No Hit
TAGATACCTAAAAACAAGAGAGGAAATTACATAAGGCAACATGCGTGGAC11.2048192771084338No Hit
TAACAAAAAAAAACAAATGAAAAAGGCTCCGAGAGCTTGCAAAATGAATA11.2048192771084338No Hit
AAAAAAAAGAACCCAAGAGCACACCACGGAATAGATCACAAGAGCACACA11.2048192771084338No Hit
TCAATAAGCGGAGCAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA11.2048192771084338No Hit
CAATGAGATTGCTAGCCCAAACTACACCGGCAAGTATACTCTTCCTGTTC11.2048192771084338No Hit
ATTCTATATATTATAATTCACCAGTTAAATTTCTCATACAAGTTGAAGAT11.2048192771084338No Hit
CCCCCAGGGACTGGTAAAACTTTAATGGCTCGTGCCTGCGCAGCACAGAC11.2048192771084338No Hit
ATCCAGCGCACGGTAGCATCGCGCCACAGGCTTTTCATCCAAGCCCCATG11.2048192771084338No Hit
GTTTAAACATACAAAGACACTGAAAACATAAAGAAACCCAATCAAAGGGA11.2048192771084338No Hit
AGCAATAGCTTACGGTCTAGACAAGAAGGCTACAAGTGTTGGTGAGAAGA11.2048192771084338No Hit
GAGAATAGTGTAAGTGTGGAAATTAGCTGTTGGATCAACCCAGAGTTTGA11.2048192771084338No Hit
GCATACCTTTAACGGAGACGTTATTATAACATAAAGGTAACTGAAAATTT11.2048192771084338No Hit
TAACTCGACGGATCGCATGGCCTCTGTGCTGGCGACGCATCATACAAATT11.2048192771084338No Hit
TTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAAGTG11.2048192771084338No Hit
AACAAGGGCCCACCCCAGGACAGCCCCGATTTCCCGCTCAGATTAACCAA11.2048192771084338No Hit
AAAAAATGTTATGGATCAAGTTCACAACCGCATGTCGGTACGCTCCAGGC11.2048192771084338No Hit
CCCTTAACTGACCACTCACTCTCCGACAACCCAAACCACATCCAAACAAC11.2048192771084338No Hit
GCTTCTAGGATCATCGGTGTTGATTTTAACTCTAAAAGAATCGACCAAGC11.2048192771084338No Hit
TAAGAAAGACGAACAACTCCGAAACCATTTGCAAAGGATTTATCATTAAT11.2048192771084338No Hit
GTAATAATAACAAGAAAACTTCTTTTAGCGGTGTTGGCTCCTCTCTCTAC11.2048192771084338No Hit
GAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGC11.2048192771084338No Hit
GTATAGTTCCCGTTAGGAGATTATTGCCGAGTTTGAGTTGAGTTAACAGA11.2048192771084338No Hit
GAAACAGAGAGCGGTTTCATCAGAGGAGCCGCCGTTGGTGCTATCTCAGG11.2048192771084338No Hit
CTCCAATGCATTGCGCAAAAGCATCCTGAATCACAGTCTCTGTTTCGATG11.2048192771084338No Hit
GGTCGGATGCGGAACGGAGCAATCCGGTCCGCCGATCGATTCGGGGCGTG11.2048192771084338No Hit
GTTCATGTACAAGCAGAAGCCTTACTAGAAGGGAATTCCTTTCCTGCCGC11.2048192771084338No Hit
GACCAAAACAGAACGACCCCCGAACCAAAGATCACCACTCTCGCAGCCCC11.2048192771084338No Hit
GATTAAAGACTACCGAGAGCTGAACCGGGACTAAACCGATTCCATCATCA11.2048192771084338No Hit
CATATATACTACAAAAGAGAATTCTCATGCTACTCCAAACCTGTAGCGGA11.2048192771084338No Hit
CAACACCCTTTGTGGGTTCTAGGTTAGAGCGCAGTTGGGCACCGTAACCC11.2048192771084338No Hit
CCAAAAAATCATCAAAAACAAAACAATTAACAAAATGACTGAGCATAAGC11.2048192771084338No Hit
CTTCCGATCTATAAAGGGGCGACCCCAGGTCAGCCCGGGATTACACGCTG11.2048192771084338No Hit
ACACAAAGAACAGAAAATAAGAAAGATAATAAAAGGAGTCAAACACGAAC11.2048192771084338No Hit
GTAACACTGTTGCTATGGTTGGGCTGGATCAGAACATCACCAAGAATGCT11.2048192771084338No Hit
GTATTAATCGAACAACACCCTATGTGTCTTCTAGGTTAGCGCTCAGAATG11.2048192771084338No Hit
ACAAACACCCTCGACCACGAAACCTCTCACACCATCCACAACGTAAACGC11.2048192771084338No Hit
GATGATTTCGGCAATCCTCCATCAAATATGGCTGCCAAAAACGAGGGATA11.2048192771084338No Hit
CTCCCTGGTGATGAACCCATGCAAGGGCTAGTTGTGCAGGAGTGCATCCT11.2048192771084338No Hit
GGATAGCTGGAGCTCGGACGCGGGTTCTATCGGGTAAAGCCAATGATTAG11.2048192771084338No Hit
AAAAACGTACTCGTATAGATCAAACGAGATCGTCTTCAAGATAATCAAAC11.2048192771084338No Hit
TTCTTAACCCAAGCTATCAGTTTCGGAACGTCTGATTGGAGACTGGAGTT11.2048192771084338No Hit
CCCAAGGGAGACGTGTGCTCTTCCGATCTCCCAACCGAGACGGGGGCTCT11.2048192771084338Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGAGAAAGGTGAAAAGCTTATCTCCCAAGACTGCAAGAAAGTCAAGGCA11.2048192771084338No Hit
GTAATGGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTGCTCC11.2048192771084338No Hit
CTGCCGCTATCCGCCACAAGAGCCGCCATGCTCGTCAGAGTCAACACTCT11.2048192771084338No Hit
GGATCATTCAATCCCTAGGAGCGACGCGCGGTGTGTACAAAGGGCAGGGA11.2048192771084338No Hit
GTCTACAGCCACAAACCATAGAATCACTTAATCTTTTGAGAAGAAGGAAT11.2048192771084338No Hit
ATGCAGATCTTCGTTAAGACTCTCACCGGAAAGACTATCACCCTCGAGGT11.2048192771084338No Hit
GTCTCAGAATCTTCGACATCAAGAAGTTGGTCACAAGAGTCTCTTACAGA11.2048192771084338No Hit
CTTCAACCACATACTTCCTTTCATCAACTCGCGGAAGAGGCATCATGATA11.2048192771084338No Hit
GACAGAAAAGTAACCACACGGATAACTGGCATCAGCCAGCCAACCGATAA11.2048192771084338No Hit
GACGAACAACTGCGAAAGCAATTGCCAAGGATGTTTTCAAAAAACAAGAA11.2048192771084338No Hit
GACCTGGAGATATCAGAAGAGAGAATGCTGACATGAGTAACGATAAATCC11.2048192771084338No Hit
AACAATAACACAACCACAGATAAATCAACAACAAACAATAACCTCACTCC11.2048192771084338No Hit
TCAATAAGCGGAGCAAAAGAAACTAACAAGGATTCCTTTAGTAACGGCGA11.2048192771084338No Hit
AATTAAAGTTAGAGAAAACCTCGACGCCGTTCCCCCCCCAATGCCACTAA11.2048192771084338No Hit
GCCTGACTCTGCTGACTATGATGACGAGGAAGAGGAGGAAGAAGAAGAAG11.2048192771084338No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACAA50.0145.0145
AAATCAT50.0145.06
AATCATC50.0145.07
CCAAAAA50.0145.01
ATCATCA50.0145.08
AAAAATC50.0145.04
TCATCAA50.0145.09
AAAAAAT50.0145.03
CAAAAAA50.0145.02
AAAATCA100.072.55
Produced by FastQC (version 0.11.5)