FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA11.1111111111111112No Hit
CAACAGAATTAATACTAAGCCACATCCCTGAATCATTAACAATGAGGATG11.1111111111111112No Hit
AATAAGAAGAAGATGATGATGATGAAGAAGAAGACGTAGATTCAGATTCC11.1111111111111112No Hit
CTCTTCCGAACTGCAAAGGGTTCTACCCGCCGCTCGGTCGTAATTATAAT11.1111111111111112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1111111111111112No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGGAATTCATAGTCAGA11.1111111111111112No Hit
ACATCATAGGATTTCGATCCTATTGTGTTCGCCATAGGCATCGGACTAAT11.1111111111111112No Hit
TATCTGGAACGCTATCAAGTTTGGAACAGTTTTGGAAAATGTTGTGTTTC11.1111111111111112No Hit
GGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTA11.1111111111111112No Hit
ACTTGAAGCAGCACGTATTGCTTGCAACAAGTATATGGTGAAATCTGCTG11.1111111111111112No Hit
ACTCTACCATCAAAATCAGAAGGTAAAAAGGATAGATAGGCGAAGCTATC11.1111111111111112No Hit
GTCCACTAACAAGAAGCAAACCAGAACAAAATAGCTAGAGGAAGACAACT11.1111111111111112No Hit
GGATGAACCGGAAGCCGGGTTACGGTGCCCACCTGCGCGCTAACCTAGAA11.1111111111111112No Hit
GGCCTAAACGGCCATAGTCCCTCTAAGAACCCCGCCCTGAAGGGATGCCT11.1111111111111112No Hit
CATATTATTTACAAGCAAGAAGCAGACATAAAAAGAGAGAGTCAGCAGCG11.1111111111111112No Hit
CAGTCAACTAAGAACTGGTACGGACAACGGGAATCCGACTGTTTAATTAA11.1111111111111112No Hit
ATCTGTAACTGTTTCTTCAATTTCAAAAAAATCTTCCGGATACATCAACA11.1111111111111112No Hit
AGAATGTACACATACCAATAACCAAACTCGAAAGAGCCCGCTATTCTTAT11.1111111111111112No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC11.1111111111111112No Hit
CTCCCGGAGCACCCTCATCCCGAGGCACGCCGTTAGGCGCGTGCTCCAGA11.1111111111111112No Hit
GTCCCATGGAACATCACCAACCAACATCCAGTCACCATCTTTATCTTCGT11.1111111111111112No Hit
GACCGGGGAGGTAGTGACAATAAATAACAATACCGGGCACTTTCCAGTCT11.1111111111111112No Hit
GCGAAAGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAACTACTACT11.1111111111111112No Hit
TCTTCGGCCTTCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGACGGACCG11.1111111111111112No Hit
CACTGGGGGGCTCGCTTCGGCGGGTCGATCCGGGCGGAGGACAGTGTCAG11.1111111111111112No Hit
ATGAAAAACAAAAAGAAAAGAAAAAAAAACATAGATTTAAAAAATCGTCA11.1111111111111112No Hit
CAACATAACTTGACCAGCTGCGGGATCAGGCTGCTGTTCTTAAACAAGAA11.1111111111111112No Hit
CCTATAAGCAACATCCGCTGATACCTGGTCGGCATCGTTTAAGGTTGAGA11.1111111111111112No Hit
CGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTC11.1111111111111112No Hit
AATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCATTCATAAA11.1111111111111112No Hit
ATGTTGACCCTATCGAGTTGGTGGTCTCGTTACCTCCACTCTGCAGGAAA11.1111111111111112No Hit
CTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAG11.1111111111111112No Hit
AACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGCGACCTATACCCGGCC11.1111111111111112No Hit
GCTAAGCCGTGCACAATTGAGACACAAGATTTTCAATGATGAATCTGTGA11.1111111111111112No Hit
CTTTTTGGGTATTAATCACTATACAAGTTACTTCATACAAGATTGTTTGA11.1111111111111112No Hit
CCATATGAGAAAACAAAGTTAATGCGTTCCGGCGCGAGTATGTTCGCAAT11.1111111111111112No Hit
AACTAGCCCCGAAAATCGATGGCGCTTAAGCGCGCGACCTATACCCGGCC11.1111111111111112No Hit
TCGTGAGACAGGATAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTTA11.1111111111111112No Hit
ATGGCATGGAGAGGAAGCATATCGAAGTCTATGAAAGAGCTTAGGATTCT11.1111111111111112No Hit
CTACTACTCGGATAACCCTAGTAATTCTACAGCAAATACGTGCAACAAAC11.1111111111111112No Hit
GTTTCATGCAACGAAGAAGGAACAGCATTTGTCGTGTCGAAAACAGCAGA11.1111111111111112No Hit
CATATAGATAGAAACATAGACGTCGACACTATATATCGTTATTGGTTAAG11.1111111111111112No Hit
AAATATTTAATATTTTTATATAATTAAAATTAAAAAAATAAAAAAAAAAA11.1111111111111112No Hit
GAATCAATGTTGGATTGCTGAGAACCTCTGGAAGTAGTGAAGGAAAAAGG11.1111111111111112No Hit
TTACATATCCGAACGCTATGTGCTCTGTGAAGATGACAAACCGCGCTGGG11.1111111111111112No Hit
ATTCTGAAGAACGCAAGAAGAAGCTATACTCAGAAAAACAGACTCTTCAA11.1111111111111112No Hit
GACAGGAGATATGAACTGCGGAGGAAAGGTGGTCTGCGTTACCGGAGCTT11.1111111111111112No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCGTAAGATCCATGCAAAG11.1111111111111112No Hit
TCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCC11.1111111111111112No Hit
CAATATAAGCAACTACCACCAAGATCTCCACCCACGGCCCCACCCCCCGG11.1111111111111112No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA11.1111111111111112No Hit
AAGTTATCACATGCTAAAGTATCCAGAGCGTAGGCTTGCTTTGAGAACTC11.1111111111111112No Hit
ATGACTCTATTAACGTAGCCAAATGCCTCGTCATCAAATTAGTGAAGCGC11.1111111111111112No Hit
GGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCGTCGGCTGTC11.1111111111111112No Hit
GGTACGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGAAATGCA11.1111111111111112No Hit
GTATAAGGTTGAGACTACGACCGTATCTGATCGTCTTCGAGCCCCCAACT11.1111111111111112No Hit
GCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCACTTAA11.1111111111111112No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA11.1111111111111112No Hit
CATATGAACCATACATTTCTCTATAGTCTTAATCTTCTTATCAAAAATCA11.1111111111111112No Hit
TCTCCAAGCTGAACAGCCTCTGGTCGATGGAACAATGTACGCAAGGCAAG11.1111111111111112No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11.1111111111111112No Hit
GAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTC11.1111111111111112No Hit
ACAGGGGAATGCAATGGATGCATGCCCCGGCATTCAGAAGGAAGCACGCT11.1111111111111112No Hit
CTATACCTTCAAGTTTGTCCCTGATCTTAATTTGGAACTGAAGAACTGCG11.1111111111111112No Hit
GTTTAACACACAAATATAAATAAGACACACATAAAACCACTTCACAGACA11.1111111111111112No Hit
GGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG11.1111111111111112No Hit
GTAGGGAATGTAAATCACGCCTTCTTCTGCGATGAACTCAAGTACTCCGC11.1111111111111112No Hit
CCCTATGACGGGAGTGCAATAGAGAACCTGAGATCCGATCCGAACAATCA11.1111111111111112No Hit
GGACGCATTTATTAGATAAAAGGTAGACGCGGGCTCTGCCCGTTGCTCTG11.1111111111111112No Hit
CCTTATACTGGAACATGGCCATAACGTTGTAGATCGTTTAACAGCTTAAC11.1111111111111112No Hit
ATCCATTTTCGGGGCTAGTAGATTCCGCAGGTGAGTTGATACACACTCCT11.1111111111111112No Hit
ACACAAGAGTTTTAAGAAAACAACTTACAAGTATCGACCACTTCCTAAGG11.1111111111111112No Hit
AATCCGATCAAACGAGACCAGTCAACAGCAATGCAATACGCATAATACTT11.1111111111111112No Hit
CGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCG11.1111111111111112No Hit
ACCCAAGGCACAAACCGACCAATCAACACCAAAGGCGGACCCGCCGACCC11.1111111111111112No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTTCACCCACC11.1111111111111112No Hit
GAAGAAGGCGGTAATGACGGAGAAGACTCAACAGCTGCTGGAGATTTTTG11.1111111111111112No Hit
ACCAATAACCCCCCGGACCCCAAAAACATAGATTTCTAAAATTATGAAAG11.1111111111111112No Hit
CCACCAGAGTTTCCTCTGGCTTTACCCCGCTCACGCATAGTTCACCATCT11.1111111111111112No Hit
GCCATGATGCACATCCCAGTCCACAATCAGCACCTTCTTTGCCCCTGCTG11.1111111111111112No Hit
GATAAAAGCTCGTAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTG11.1111111111111112No Hit
GAGGATTACTTAGGCTTATAGGGTTGTCCCCCTTTCTCGCGTCACAGCGA11.1111111111111112No Hit
TTTGTCACAAGCAACCCAATGTTCTTGTATGATCACCGGATTTGCTTACG11.1111111111111112No Hit
AACAGGGACAGTCGGGGGCATTCGTATTACATAGTCAGAGGTGAAATTCT11.1111111111111112No Hit
CTCTTTTCAAACTCCTTATCAACTTTACCTCGCGCTACTTCTAAGCTATC11.1111111111111112No Hit
TAATATAAGATCGTGTGATTGGACCCCCAGATGTTTGCTAAAAACAAAAA11.1111111111111112No Hit
GCATCAATACATTATTGCCACCCTCAAATGTAGTCTGCACATCAAATTCA11.1111111111111112No Hit
ACACAATACGATCGAAATCCTATGATGTTATCCCATGCTAAAGTATCCAG11.1111111111111112No Hit
GATCTGCAGTGGGCAGAAATCACATTGCGTTAGCATCCGAAGGCACCATC11.1111111111111112No Hit
CCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGC11.1111111111111112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC50.0145.01
GTACTCA50.0145.04
AACCCGG50.0145.0145
ACTCATA50.0145.06
CGTACTC50.0145.03
TCGTACT50.0145.02
CTCATAA50.0145.07
TACTCAT50.0145.05
TCATAAC50.0145.08
CATAACC50.0145.09
Produced by FastQC (version 0.11.5)