FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6B1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6B1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCTTATTGTCTTCGGTGGGCTTGACCCGATTACGTGGCAGGCCCATG12.5No Hit
CTATCCTTCCTTCTGATCCTTACCTGAGAGCTCAAGCTAGGTTCAGCGCT12.5No Hit
GACAACAATTCGAACGCCGAAGCCGTCCGATTTTCAAGCTGGGCTCTTCA12.5No Hit
CTATTGCTCCCTGAACAATCCAACACTTGGAGAAATCAGCATCACAATGA12.5No Hit
AAATACTCCTCCCACGGCCACTTTTGTAAGCACCAGAATTCCTGCTACAG12.5No Hit
GTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGATCCCGAGAGAGCCGTT12.5No Hit
ATCTAAATCCATTAACGAGGATCCATAGGACGGCAAGTCTCGAGCAAGCA12.5No Hit
AAACAAAACCAAGACTCCAGGTCCTGCTGCTCAGACTGCTCTTAGAGCCC12.5No Hit
GAAGAGGAAAGAAAAGAAGATTTCAATTCTTATAACTTGCAGCCGTTTCT12.5No Hit
CACACACACTCAAGTAAAACATGAACCATAACCATGACAAAAACAAAAGC12.5No Hit
CTCTTTGACTCTCTTTACAAAGTCCTTGACATCTTTACCTCCCCGTACAA12.5No Hit
CTAATGGAGCTGGAAATACCCCGGCTGCTGCCACCAGACTTGCTCTCCAA12.5No Hit
GTATAAAAAGCCTCGAGTTTCATGATAACAAGCCACGAGATTGCTTACTT12.5No Hit
GAAAGACACCGCTACTCGCAGCAGTGTACGTTGGTGCCCTCCAGAATATC12.5No Hit
CTTGGGCTCCTTCTCAATCTCCATACCAGAACGCCTGTCAATCTTGGTCA12.5No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTCTG12.5No Hit
TTATAGAACAGCTCAGGAAGAGGACCAGGTCGGTGCTGAGGAACATGCAT12.5No Hit
ATAAAAGACAAACCACACCAACAACAGCCTGATGCCCCACCACACCTTCA12.5No Hit
CATAAAAGCAAAATCCCCAGATACATGGTCGTCATATTTTATGTTTGATA12.5No Hit
GCTCTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGCCGTCTCC12.5No Hit
AACCCGATACTAAGAAGAACATGAAGAAACAGAAGATGAAGCAAGGCGAC12.5No Hit
GTTCTGCATCAGAATGAAAATCCCGCAAGCGGTAAACGTATTGAACAACA12.5No Hit
ATATGGAACGGCTGTAATGCAAAAAGAGGTTAATCCCGCTCGTAATGTAT12.5No Hit
AATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGAT12.5No Hit
CACATAACGTCCCAGCGGAGTCATATAATCAACAACAGCATAACACTGAT12.5No Hit
TAGTAGAACACTTATTAATCAGGGATTATACAAGGACCCAAAATACAAAC12.5No Hit
CCCCAGATCGTTACGCCTATCGTGCGGGTCCGAACTTACCCGACAAGGAA12.5No Hit
CCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGG12.5No Hit
GCCTATCGATCCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAAA12.5No Hit
CAAAGAGGGTATCCCACCGGACCAGCAGAGATTGATCTTCGCCCGAAAGC12.5No Hit
GATTGATCTCACGTAGGAATATGCTGCTTCAGGTGACATGTATTTGTGTT12.5No Hit
ACAAACCTAACCTCAAAGCCAACCAAAAAGAAAACAATCACTAACCAATG12.5No Hit
GTTGGAAAAAGTTGGCGGGTGGGGTGCCCTGGGCCAGGTCGATGATGCGG12.5No Hit
CAGCAGCACCGGCTCCACGTAGTCTGAATCGAATTTTTCAGTTGGCTTTC12.5No Hit
CTTCTGAATTATTCATCGTGGAGATTCTCGGATCGGGAAGGGCTGCGAGA12.5No Hit
CCTACTGGCTTCACGCAATTCCTTTCAATGACACCTATGCCTCCGAAACT12.5No Hit
GATATAAATCTCAAAACAACAAAATCATCGAACAGACCAGACAAATCAAC12.5No Hit
CCAAAAGACAACCCTTTTCGTGAAGACAAACAGCTAGAACCTGAAATATG12.5No Hit
GGCCTACACAATGCCTCCAAATGCACAACACATCACTGTTCTTCGTGTGG12.5No Hit
ACTAACAGCTGTAGGATCACACTGTTCTTTATCTGAGATGTATGATCTTG12.5No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers