FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6B12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6B12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACGAGATCAAGGGCCCCTAGTCCCGTATTTAAGACTACCCGGCTGAGT11.5151515151515151No Hit
CGTCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACGCAAAACC11.5151515151515151No Hit
GTACCCCGCCTCCGATTCACGGAGTAAGTAAAATAACGTAAAAAGTAGTG11.5151515151515151No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT11.5151515151515151No Hit
CAATAGGATCGGAATCCTATGATGTTATCCCATGCTAATCTATCCAGAGC11.5151515151515151No Hit
GACAAGGGGAAACCGACTGTTTAATTAAAACAAAGCAATGCGAAGCTCCC11.5151515151515151No Hit
TAAATTAAAAAATTAATAATTTATTATTATAAAAAAAAAATTTTTTTTTT11.5151515151515151No Hit
AAACTTAACAGAGGGTAAAAAACTCTCTCTAATGAGGTCAATATGAGTAA11.5151515151515151No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGCATTGTTGAGCCGTCACACGGA11.5151515151515151No Hit
GGTGAGAAAACCCGTAAGGCGGAACCAAGCTGATTGGCGGGATCCCTCGC11.5151515151515151No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTAAAGGGGCGAAAGACT11.5151515151515151No Hit
GTTAAAAGATATCGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAAT11.5151515151515151No Hit
GTGTTGGACTCAAGGGTCCTCTTGACAATACAGAAAGCGTCATGCAGTGC11.5151515151515151No Hit
AAATAACAATACCGGGCTCTATCGAGTCACGATATTGGAATCAGTACAAT11.5151515151515151No Hit
CGTAGACAGCGCCTCGTGGTGCGACAGGCTCCGGGCACGACGGGGCTCTC11.5151515151515151No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG11.5151515151515151No Hit
GGGTAAAACCAGAGGAAACTCTGGTGGAAGCCCACAGCGATACTGACGTG11.5151515151515151No Hit
AAGATACGAAACAGCCTCAGGTCTCTTTACTTCACCGAATAACCGAGACA11.5151515151515151No Hit
GACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTA11.5151515151515151No Hit
GTTCGGGGGCGACCCCAGGTCAGGCGGGATTACCAGCTGAGTTTAAGCAT11.5151515151515151No Hit
GTTATACCCCTGCCTAAGTATGAGAAAGCGGTTTATCACACTCGCGACTC11.5151515151515151No Hit
CTTCCGATCTGCACCGGGGAATGCATTGGATGGATGCCCGGGGATTGAGA11.5151515151515151No Hit
GTCACGACCCGGCCAATTAAGACCAGGAGCGTATCGCCGACCGAAGGGAC11.5151515151515151No Hit
CATTAAATCAGTTATAGTTAGTTTGATGGTAACTACTACTCGGATAACCG11.5151515151515151No Hit
GGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAAACCGTA11.5151515151515151No Hit
CCACCAAGATCTGCACCGACCGCCGCTCCGCCCGCGCTCGCGCGCTACGT11.5151515151515151No Hit
GGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCC11.5151515151515151No Hit
CTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTATT11.5151515151515151No Hit
CCTATACCCAAGTCAGACCAACGATTTGCACCTCAGAATCGCTCCGGGCT11.5151515151515151No Hit
TTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGAAATC11.5151515151515151No Hit
GTAGTCTGGAGAAGCGTCCTCAGCGACGGCCCGGGCCTAAGTTCCCTGGA11.5151515151515151No Hit
CTAAAACACACACGTTAATCACACCTGAACATCGGTCCATATATTTACTT11.5151515151515151No Hit
CATCAAAGTAACAGCGCCGCACGCACGACCCGGACAATAAAGACCACGAG11.5151515151515151No Hit
TAAATAGCCTTGGACGGAATTTACCGCCCGAAAGCGGCACCATTCCCAAA11.5151515151515151No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGTTCCCTA11.5151515151515151No Hit
GGCCTAAGTTCCCTGGAAAGTGGCGCCAGAGAGGGTGAGAGCCCCGTCGT11.5151515151515151No Hit
GGATAACATCATAGGATTTCGATCCTATAGTGTTGGCCTTCGGGATCGGA11.5151515151515151No Hit
TAACAAGACGGGTCGAATGGCGAGCCCAAAAGCCGACGCCCGGACCACGC11.5151515151515151No Hit
GTCTCCCCAGGTCAGATCGGAAGAGCACACGTCTCCCAAGGTCAGATCGG11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCGTCCTAGTCTCAACCAAAAACGAAGCCGACCACGGATCAGCGGATGTT11.5151515151515151No Hit
CGATTAGTCTTTCGCCCCTAAACCCAAGTCAGACGAACGATTAGCACGTC11.5151515151515151No Hit
GCGCCCTCCTACTCAACGAGGCCTCGCTCTTGCCCCCCCGGCCGGGTATA11.5151515151515151No Hit
TGCTACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCCGCGGTGCACC11.5151515151515151No Hit
ACTCAAGCCATCAAGCTCGATCCTTCTAAAGCTACTCATTACAGAAACCG11.5151515151515151No Hit
GTAGAAGAGAGCTCTAACACGCCATGGACGACCGAACCCACGAATGACCC11.5151515151515151No Hit
ATCTAAATCACTTAACGAGGATCCATTCGAGCGCAAGACTGCTGCCAGAA11.5151515151515151No Hit
ACCTAAAGATCAAAAGGACTTTGAAAACAGAGACAAAGAGTGCTTGAAAT11.5151515151515151No Hit
GCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCAGTCGCACCACG11.5151515151515151No Hit
GCCTTGCGACCATACTCCCCCCGGAACCCAAAAACTTAGATTTCTCATAA11.5151515151515151No Hit
AATATGCCGACTACCCTTGCCTACATAGTTCCATCGACCAGAGGCTGTTC11.5151515151515151No Hit
TGCAAGACCTCTTCAAGGAATTTGGATGTGAATCAAAGCTTAGAGTTGAT11.5151515151515151No Hit
GACGGAAGGGCACCACAAGGAGTGGAGCCTGCCGCTTAATTTGAAACAAA11.5151515151515151No Hit
TGCCTGTCGGGCCCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGC11.5151515151515151No Hit
TTCTTAATCGACAAACACACATTGTGGTATCTATGTTACAGATCATATCG11.5151515151515151No Hit
TTTAAACAGAGACTAGGATAGCAATAAGCACTCAAATTTCTTCAAAGTAA11.5151515151515151No Hit
GCCTTGCGACCATACTCCCCCCGGAGCCCAAAATCTTTAATTTCTCATAA11.5151515151515151No Hit
AAATTCAACCAAGCGCGGGAAAAACCGCCGGAGAAACTATGACTCACATA11.5151515151515151No Hit
GATCATTCATAATAAATAGACCGATCGCATGGCCACTGACCTGAAGACGC11.5151515151515151No Hit
CCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACTCCG11.5151515151515151No Hit
CTGATGCCGACGCACGCCGTTAGGCGCGTGCTGCACCCCACGATCACGGC11.5151515151515151No Hit
GATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGG11.5151515151515151No Hit
GGGTGCCTGGTGGCGGCTGCCATGGCTGCCGCTCAGCTGGGTGGGCAGGG11.5151515151515151No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC11.5151515151515151No Hit
AAGCATATCAATAAGCGCAGGAAAAGAAACTAACAAGGATTCCCTTAGTA11.5151515151515151No Hit
GTGGCGACACAGCACAGAGCCCCGACAAGCCTTGGCACGCCTGCCCTAAA11.5151515151515151No Hit
GGGAGCTACCGTATGTGATCCATGGATCTCCGATCGGGAAACCGTATCCA11.5151515151515151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGA50.0145.04
AACGATG50.0145.06
GATGCCG50.0145.09
GGGGGGG50.0145.0145
ATAAACG50.0145.03
CCATAAA50.0145.01
ACGATGC50.0145.07
CGATGCC50.0145.08
AAACGAT50.0145.05
CATAAAC50.0145.02
Produced by FastQC (version 0.11.5)