FastQCFastQC Report
Tue 11 Sep 2018
H55FTAFXY_n01_p6B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAACTCCCCTACAACCAAACAGTTCAAATCCAACTTCGTTACACAGCC19.090909090909092No Hit
GGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGT19.090909090909092No Hit
CTAAAAGATAATTATACACAAAGTATAACCGATATTTCGAAAGTATATTT19.090909090909092No Hit
TCCCTCTAGAGATCGGAAGAGCTCACGTCTCCCTCAAGAGATCGGATGAG19.090909090909092Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTGATACCGATGCGGCGGAGAGCAGCGAGTGCATCTGCATGCGGCTGAC19.090909090909092No Hit
ACTGAGAAGGGTTCGAGTGAGAGCATGACTGTCGGGAACCTAAAGATGGT19.090909090909092No Hit
CTTCCGATCTAAGCTGGGTGTGAGTCTGCTGCTGTTTGTTCCCGCCTATG19.090909090909092No Hit
CCGCATTCCAATGGTTGAAGCATAAAGGAGAACTTATATGAATATTGTAC19.090909090909092No Hit
GGCTTATGAGCAATGATGCTCCTTGATCCGTTATGCTCAAGTTCTTGTAT19.090909090909092No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19.090909090909092No Hit
CTTATACACATATCCGAGCCCACGAGCCGCTCATGAATGGGGGGTAGAGC19.090909090909092No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)