FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_wt-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_wt-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11403736
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4695564.117562875885588No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC540020.4735465640383117Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38550.051.1100518
GAGCACA76400.046.77068316
AGAGCAC77900.046.09613815
AGCACAC51550.042.36365517
CACACGA30650.040.4182919
ACACGAG31450.039.38929720
CACGAGA35750.034.7465521
AAGAGCA156000.030.85030414
CACACGT26250.028.92914219
CACGTAG24750.027.42748321
GCACAAG49150.026.62995118
AAGGGGG102300.026.57984467
AAAGGGG105400.025.83230666
CCGTATC185300.025.07990649
CGCCGTA193950.024.60994747
ACACGTA28900.024.33850120
ACGAGAT52200.024.0644422
GCCGTAT206600.023.86529248
CGTATCA197550.023.64860550
CGAGATC53150.023.30504423
Produced by FastQC (version 0.11.5)