FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11278287
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4268033.7842892276105404No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC352000.3121041342537213Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG46100.050.78569818
GAGCACA74000.046.16054516
AGAGCAC78450.044.65983215
AGCACAC59850.042.4529617
ACACGAG29850.039.3894920
CACACGA30450.038.49961519
CACACGT32200.036.62460319
CACGAGA34050.034.42566321
CACGTAG27200.032.93257521
AAGAGCA152750.028.9189814
ACACGTA33450.026.7811520
CAGATCG108250.026.5343656
CGAGATC47950.025.32162923
ACGAGAT51500.023.03281822
TAGATCG110450.021.6926786
CGCCGTA143600.021.39413547
CCGTATC141150.021.32124749
CGTAGAT43450.021.2600823
GCCGTAT153650.020.72342348
GCACAAG38850.020.26785318
Produced by FastQC (version 0.11.5)