FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296809
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77309712.277599654046995No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC117520.1866342142504243Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77590.12322114264542565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG108400.065.0149318
AGAGCAC129850.063.5459515
AGCACAC109550.063.44151717
CACACGT82350.063.28142519
CACGTAG39450.061.86839721
ACGTGTG68850.060.70981616
GGAGTTC70600.060.6687367
TAACTGA92350.060.595922
CGTGTGC68500.060.5594917
GTGACTG64250.060.024221
ACTGGAG68550.060.016974
CAGACGT70100.059.92426313
TGACTGG66600.059.7694742
TCAGACG71600.059.64681612
CACGTCA44550.059.57369221
AGACGTG71150.059.49039514
GTGTGCT67400.059.31352218
ACACGTC45950.059.2162320
ACACGTA40300.058.92659820
ACTGAAG93950.058.027724
Produced by FastQC (version 0.11.5)